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Invitrogen
CSF2RB Chimeric Recombinant Rabbit Monoclonal Antibody (BION-1)
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FIGURE: 1 / 2
CSF2RB Chimeric Antibody (MA5-48120) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of CD131 (CSF2RB) in U937 cells using CSF2RB Chimeric Monoclonal Antibody (Product # MA5-48120). Paraformaldehyde-fixed cells were immobilized on cover slips and stained with CSF2RB Chimeric Monoclonal Antibody at 10 µg/mL followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from lef... View More
Product Details
MA5-48120
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG, kappa
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
BION-1
Immunogen
BALB/c mice were immunized intraperitonally with COS cells transfected DQP (Domain 4 of the common beta-chain) expression constructs.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.02% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3074563
Product Specific Information
Specificity: Binds to domain 4 of the common beta-chain and recognises the same binding site used by IL-5, GM-CSF and IL-3, or binds to an epitope in close proximity to it. Residues M363, R364, E366 (B-C loop) and R418 (F-G loop) in the common beta-chain are important for BION-1 binding.
Preparation: BALB/c mice were immunized intraperitonally with COS cells transfected DQP (Domain 4 of the common beta-chain) expression constructs. The immunizations were repeated 4 times at 2-week intervals. Four weeks after the final immunization, a mouse was boosted with COS transfectants intravenously. Three days later, splenocytes were harvested and fused with NS-1 myeloma cells. Hybridoma supernatants were screened on CHO DQP cells by flow cytometry, with untransfected CHO cells as a control. All antibodies were from single hybridoma clones as selected by a limiting dilution method. MoAbs were purified from ascites fluid or hybridoma supernatant by a protein A sepharose column.
Target Information
The protein encoded by this gene is the common beta chain of the high affinity receptor for IL-3, IL-5 and CSF. Defects in this gene have been reported to be associated with protein alveolar proteinosis (PAP).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: beta common cytokine receptor; CD antigen CD131; CD131; CDw131; colony stimulating factor 2 receptor, beta, low-affinity (granulocyte-macrophage); colony-stimulating factor-2 receptor, beta, low-affinity; Cytokine receptor common subunit beta; GM-CSF/IL-3/IL-5 receptor common beta subunit; GM-CSF/IL-3/IL-5 receptor common beta-chain; IL-3 RB; IL-3 Receptor beta; IL3R beta; interleukin 3 receptor/granulocyte-macrophage colony stimulating factor 3 receptor, beta (high affinity)
Gene Aliases: CD131; CDw131; CSF2RB; IL3RB; IL5RB; SMDP5
UniProt ID: (Human) P32927
Entrez Gene ID: (Human) 1439
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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