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CTCF Antibody (MA5-49543) in WB
Western blot analysis of CTCF in various protein samples. Samples were incubated with CTCF monoclonal antibody (Product # MA5-49543) with a dilution of 1 µg/mL, followed by peroxidase conjugated goat anti-rat IgG secondary antibody. Ctcf band was visualized using ECL Western Blotting Substrate. Lane 1: 15 µg of HeLa lysate; Lane 2: 15 µg of Jurkat lysate; Lane 3: 15 µg of MOLT-4 lysate; Lane 4: 15 µg of NIH/3T3 lysate. Result: MA5-49543 can detect Ctcf by Western blotting.
Product Details
MA5-49543
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rat
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
KT71
Immunogen
Recombinant Mouse Ctcf
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_3093301
Product Specific Information
This product was used in an Indirect ELISA.
Target Information
Transcriptional factor CTCF is a ubiquitously expressed 11-zinc finger domain containing, DNA-binding nuclear phosphoprotein that is involved in multiple aspects of normal gene regulation including transcriptional repression and activation, gene silencing, chromatin insulation and regulation of imprinted sites. CTCF uses different combinations of ZF domains and interacts with the CCCTC motif on the DNA. It can bind to HAT- and HDAC-containing complexes thereby influencing transcriptional activation or repression respectively. It is a multifunctional protein involved in the transactivation of APPB gene, silencing of c-myc gene, and insulation of human beta-globin and DMI myotonic dystrophy locus and imprinting control of IGF2 and H19 regions. CTCF has also been shown to regulate the transcription of Pax6 and IRAK2 promoter thereby opening a therapeutic avenue. Mutations in this gene have been associated with invasive breast cancers, prostate cancers and Wilms' tumors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 11 zinc finger transcriptional repressor; 11-zinc finger protein; CCCTC-binding factor; CCCTC-binding factor (zinc finger protein); CTCFL paralog; OTTHUMP00000197610; Transcriptional repressor CTCF
Gene Aliases: AW108038; CTCF; MRD21
UniProt ID: (Human) P49711, (Mouse) Q61164
Entrez Gene ID: (Human) 10664, (Mouse) 13018
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
transcription corepressor activity
protein binding
zinc ion binding
chromatin insulator sequence binding
sequence-specific DNA binding
transcription regulatory region DNA binding
nucleic acid binding
DNA binding
chromatin binding
metal ion binding
negative regulation of transcription from RNA polymerase II promoter
DNA methylation
regulation of gene expression by genetic imprinting
transcription from RNA polymerase II promoter
chromosome segregation
maintenance of DNA methylation
positive regulation of gene expression
covalent chromatin modification
nucleosome positioning
regulation of histone methylation
regulation of histone acetylation
regulation of gene expression, epigenetic
regulation of molecular function, epigenetic
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
regulation of centromeric sister chromatid cohesion
transcription, DNA-templated
regulation of transcription, DNA-templated
dosage compensation by inactivation of X chromosome
negative regulation of gene expression
chromatin modification
Performance Guarantee
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