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CTNNA3 Antibody (MA5-42488) in IHC (P)
Immunohistochemistry (Paraffin) analysis of paraffin-embedded mouse heart tissue using CTNNA3 Recombinant Monoclonal Antibody (Product # MA5-42488). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the CTNNA3 antibody at a ... View More
Product Details
MA5-42488
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JE55-32
Immunogen
Recombinant fragment within C-terminal Human CTNNA3
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2911629
Product Specific Information
Positive Control: Rat heart tissue lysate, mouse heart tissue lysate, 293 cell lysate, mouse heart tissue, rat heart tissue.
Subcellular Location: Cytoskeleton.
Target Information
Catenins are proteins that connect cadherin/beta-catenin cell adhesion complexes to the actin cytoskeleton. Although originally discovered in testis, alphaT-catenin is expressed in other tissues, the highest levels being observed in heart. Immunohistochemical analysis showed human alphaTcatenin localization at intercalated discs of cardiomyocytes and in peritubular myoid cells of testis. It is proven that alphaT-catenin can functionally restore cell-cell adhesion in colon cancer cells lacking alpha-catenins. This indicates that this protein is involved in the formation of specific cell-cell contacts in specific types of muscle cells. The encoded protein has about the same predicted size (100 kDa) as other alpha-catenins to which it shows an overall amino acid identity of 57%. In cells transfected with alphaT-catenin cDNA, interaction with alpha-catenin was demonstrated by coimmunoprecipitation. The 892_24D2S antibody against alphaT-catenin does not show any cross reaction with alphaE-catenin or alphaNcatenin in western blot analysis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Alpha T-catenin; Alpha-3 catenin; alpha-catenin-like protein; alpha-T-catenin; Cadherin-associated protein; catenin (cadherin associated protein), alpha 3; catenin (cadherin-associated protein), alpha 3; catenin alpha 3; Catenin alpha-3; CTNNA3
Gene Aliases: 4930429L08Rik; 4933408A16; ARVD13; Catna3; CTNNA3; RGD1559455; RGD1562230; VR22
UniProt ID: (Human) Q9UI47, (Mouse) Q65CL1
Entrez Gene ID: (Human) 29119, (Mouse) 216033, (Rat) 361839
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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