Proteintech

CUL3 Polyclonal Antibody

CUL3 Antibody in Immunocytochemistry (ICC/IF)

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CUL3 Antibody (11107-1-AP) in ICC/IF

Immunofluorescent analysis of HEK-293 cells using 11107-1-AP (CUL3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

CUL3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

11107-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 15 publications 15 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

CUL3 Fusion Protein Ag1555 (469-768 aa encoded by BC039598)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: KLEGMFRDM SISNTTMDEF RQHLQATGVS LGGVDLTVRV LTTGYWPTQS ATPKCNIPPA PRHAFEIFRR FYLAKHSGRQ LTLQHHMGSA DLNATFYGPV KKEDGSEVGV GGAQVTGSNT RKHILQVSTF QMTILMLFNN REKYTFEEIQ QETDIPEREL VRALQSLACG KPTQRVLTKE PKSKEIENGH IFTVNDQFTS KLHRVKIQTV AAKQGESDPE RKETRQKVDD DRKHEIEAAI VRIMKSRKKM QHNVLVAEVT QQLKARFLPS PVVIKKRIEG LIEREYLART PEDRKVYTYV A (469-768 aa encoded by BC039598)

Target Information

Cullin 3 is a member of the family of human cullin genes (CUL 1, 2, 3, 4a, 4b, and 5) homologous to the S. cerevisiae cdc 53 gene. CUL 1 forms a complex with human p19 skp1 and F box protein p45 skp2. Together with cdc 53 (Known as Cul A), as three subunits, they form a ubiquitin ligase-controlling proteolysis of G1 cell cycle regulatory proteins. In contrast to CUL 1, CUL 2 does not associate with p19 skp1 and p45 skp2. Instead, CUL 2 forms a complex with an inactive transcriptional elongation complex, SIII, formed by three subunits: Elongain C, Elongin B and VHL. The function of this association remains unknown.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

DDRGK1 Enhances Osteosarcoma Chemoresistance via Inhibiting KEAP1-Mediated NRF2 Ubiquitination.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Wang X,Zhou T,Yang X,Cao X,Jin G,Zhang P,Guo J,Rong K,Li B,Hu Y,Liu K,Ma P,Qin A,Zhao J

Mon May 01 00:00:00 UTC 2023

Norcantharidin promotes cancer radiosensitization through Cullin1 neddylation-mediated CDC6 protein degradation.

Molecular carcinogenesis

Deng T,Zhu Q,Xie L,Liu Y,Peng Y,Yin L,Gao Y,Cao T,Fu Y,Qi X,Zhang S,Peng Y,Hou Y,Li X

Mon Aug 01 00:00:00 UTC 2022

Tumor suppressor DCAF15 inhibits epithelial-mesenchymal transition by targeting ZEB1 for proteasomal degradation in hepatocellular carcinoma.

Aging

Dong X,Han Y,Zhang E,Wang Y,Zhang P,Wang C,Zhong L,Li Q

Sun Apr 04 00:00:00 UTC 2021

SQSTM1/ p62 oligomerization contributes to Aβ-induced inhibition of Nrf2 signaling.

Neurobiology of aging

Guo C,Zhang Y,Nie Q,Cao D,Wang X,Wan X,Liu M,Cui J,Sun J,Bai Y,Li L

Mon Feb 01 00:00:00 UTC 2021

USP15 participates in DBP-induced testicular oxidative stress injury through regulating the Keap1/Nrf2 signaling pathway.

The Science of the total environment

Zhang L,Gao X,Qin Z,Shi X,Xu K,Wang S,Tang M,Wang W,Gao S,Zuo L,Zhang L,Zhang W

Fri Aug 20 00:00:00 UTC 2021

Tangeretin maintains antioxidant activity by reducing CUL3 mediated NRF2 ubiquitination.

Food chemistry

Wang Y,Jin R,Chen J,Cao J,Xiao J,Li X,Sun C

Wed Dec 15 00:00:00 UTC 2021

Arsenic trioxide (ATO) induced degradation of Cyclin D1 sensitized PD-1/PD-L1 checkpoint inhibitor in oral and esophageal squamous cell carcinoma.

Journal of Cancer

Zhu G,Li X,Li J,Zhou W,Chen Z,Fan Y,Jiang Y,Zhao Y,Sun G,Mao W

Fri Oct 16 00:00:00 UTC 2020

Arsenic trioxide (ATO) induced degradation of Cyclin D1 sensitized PD-1/PD-L1 checkpoint inhibitor in oral and esophageal squamous cell carcinoma.

Journal of Cancer

Zhu G,Li X,Li J,Zhou W,Chen Z,Fan Y,Jiang Y,Zhao Y,Sun G,Mao W

Fri Oct 16 00:00:00 UTC 2020

Rice protein stimulates endogenous antioxidant response attributed to methionine availability in growing rats.

Journal of food biochemistry

Wang Z,Cai L,Li H,Liang M,Zhang Y,Wu Q,Yang L

Fri May 01 00:00:00 UTC 2020

KLHL18 inhibits the proliferation, migration, and invasion of non-small cell lung cancer by inhibiting PI3K/PD-L1 axis activity.

Cell & bioscience

Jiang X,Xu Y,Ren H,Jiang J,Wudu M,Wang Q,Guan J,Su H,Zhang Y,Zhang B,Guo Y,Hu Y,Jiang L,Liu Z,Wang H,Cheng Y,Sun L,Qiu X

Fri Nov 27 00:00:00 UTC 2020

The Deubiquitinating Enzyme Ataxin-3 Regulates Ciliogenesis and Phagocytosis in the Retina.

Cell reports

Toulis V,García-Monclús S,de la Peña-Ramírez C,Arenas-Galnares R,Abril JF,Todi SV,Khan N,Garanto A,Costa MDC,Marfany G

Tue Nov 10 00:00:00 UTC 2020

Rice Protein Exerts Endogenous Antioxidant Capacity via Methionine Sulfoxide Reductase and the Nrf2 Antioxidant System Independent of Age.

Journal of medicinal food

Li H,Liang M,Wang Z,Zhang Y,Wu Q,Yang L

Mon Jun 01 00:00:00 UTC 2020

l-Methionine activates Nrf2-ARE pathway to induce endogenous antioxidant activity for depressing ROS-derived oxidative stress in growing rats.

Journal of the science of food and agriculture

Wang Z,Liang M,Li H,Cai L,He H,Wu Q,Yang L

Thu Aug 15 00:00:00 UTC 2019

l-Arginine induces antioxidant response to prevent oxidative stress via stimulation of glutathione synthesis and activation of Nrf2 pathway.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

Liang M,Wang Z,Li H,Cai L,Pan J,He H,Wu Q,Tang Y,Ma J,Yang L

Tue May 01 00:00:00 UTC 2018

l-Arginine induces antioxidant response to prevent oxidative stress via stimulation of glutathione synthesis and activation of Nrf2 pathway.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

Liang M,Wang Z,Li H,Cai L,Pan J,He H,Wu Q,Tang Y,Ma J,Yang L

Tue May 01 00:00:00 UTC 2018

Increased Expression of Cullin 3 in Nasopharyngeal Carcinoma and Knockdown Inhibits Proliferation and Invasion.

Oncology research

Zeng R,Tan G,Li W,Ma Y

Fri Jan 19 00:00:00 UTC 2018

Increased Expression of Cullin 3 in Nasopharyngeal Carcinoma and Knockdown Inhibits Proliferation and Invasion.

Oncology research

Zeng R,Tan G,Li W,Ma Y

Fri Jan 19 00:00:00 UTC 2018

Increased Expression of Cullin 3 in Nasopharyngeal Carcinoma and Knockdown Inhibits Proliferation and Invasion.

Oncology research

Zeng R,Tan G,Li W,Ma Y

Fri Jan 19 00:00:00 UTC 2018

H₂S regulates endothelial nitric oxide synthase protein stability by promoting microRNA-455-3p expression.

Scientific reports

Li XH,Xue WL,Wang MJ,Zhou Y,Zhang CC,Sun C,Zhu L,Liang K,Chen Y,Tao BB,Tan B,Yu B,Zhu YC

Tue Mar 21 00:00:00 UTC 2017

H₂S regulates endothelial nitric oxide synthase protein stability by promoting microRNA-455-3p expression.

Scientific reports

Li XH,Xue WL,Wang MJ,Zhou Y,Zhang CC,Sun C,Zhu L,Liang K,Chen Y,Tao BB,Tan B,Yu B,Zhu YC

Tue Mar 21 00:00:00 UTC 2017

Bioinformatics

Protein Aliases: CUL-3; Cullin-3; OTTHUMP00000203839; OTTHUMP00000203840

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Gene Aliases: AI467304; AW146203; CUL-3; CUL3; KIAA0617; mKIAA0617; PHA2E

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UniProt ID: (Human) Q13618, (Mouse) Q9JLV5

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Entrez Gene ID: (Human) 8452, (Mouse) 26554, (Rat) 301555

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Function(s)

ubiquitin-protein transferase activity protein binding cyclin binding POZ domain binding ubiquitin protein ligase binding protein homodimerization activity protein heterodimerization activity ubiquitin protein ligase activity molecular_function

Process(es)

negative regulation of transcription from RNA polymerase II promoter protein polyubiquitination mitotic cell cycle cell morphogenesis in utero embryonic development trophectodermal cellular morphogenesis regulation of transcription from RNA polymerase II promoter ubiquitin-dependent protein catabolic process protein monoubiquitination transport ER to Golgi vesicle-mediated transport mitotic metaphase plate congression integrin-mediated signaling pathway gastrulation negative regulation of cyclin-dependent protein serine/threonine kinase by cyclin degradation Wnt signaling pathway vesicle-mediated transport cell migration protein ubiquitination stem cell division positive regulation of cytokinesis negative regulation of Rho protein signal transduction embryonic cleavage protein ubiquitination involved in ubiquitin-dependent protein catabolic process stress fiber assembly proteasome-mediated ubiquitin-dependent protein catabolic process fibroblast apoptotic process positive regulation of mitotic metaphase/anaphase transition COPII vesicle coating liver morphogenesis G1/S transition of mitotic cell cycle MAPK cascade cell cycle arrest positive regulation of cell proliferation cell projection organization negative regulation of canonical Wnt signaling pathway intrinsic apoptotic signaling pathway biological_process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CUL3 Polyclonal Antibody

CUL3 Antibody (11107-1-AP) in ICC/IF

Product Details

11107-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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