Invitrogen

CUL3 Polyclonal Antibody

View all (19) Cullin 3 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-114434

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.5 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human Cullin 3 (426-444aa DVFERYYKQHLARRLLTNK).
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 5mg BSA

Contains

0.05mg sodium azide, 0.05mg thimerosal

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2884866

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: human Hela whole cell, human K562 whole cell, human Ramos whole cell, rat brain tissue, mouse brain tissue, mouse NIH/3T3 whole cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Information

Cullin 3 is a member of the family of human cullin genes (CUL 1, 2, 3, 4a, 4b, and 5) homologous to the S. cerevisiae cdc 53 gene. CUL 1 forms a complex with human p19 skp1 and F box protein p45 skp2. Together with cdc 53 (Known as Cul A), as three subunits, they form a ubiquitin ligase-controlling proteolysis of G1 cell cycle regulatory proteins. In contrast to CUL 1, CUL 2 does not associate with p19 skp1 and p45 skp2. Instead, CUL 2 forms a complex with an inactive transcriptional elongation complex, SIII, formed by three subunits: Elongain C, Elongin B and VHL. The function of this association remains unknown.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CUL-3; Cullin-3; OTTHUMP00000203839; OTTHUMP00000203840

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Gene Aliases: AI467304; AW146203; CUL-3; CUL3; KIAA0617; mKIAA0617; PHA2E

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UniProt ID: (Human) Q13618, (Mouse) Q9JLV5

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Entrez Gene ID: (Human) 8452, (Mouse) 26554, (Rat) 301555

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Function(s)
ubiquitin-protein transferase activity protein binding cyclin binding POZ domain binding ubiquitin protein ligase binding protein homodimerization activity protein heterodimerization activity ubiquitin protein ligase activity molecular_function
Process(es)
negative regulation of transcription from RNA polymerase II promoter protein polyubiquitination mitotic cell cycle cell morphogenesis in utero embryonic development trophectodermal cellular morphogenesis regulation of transcription from RNA polymerase II promoter ubiquitin-dependent protein catabolic process protein monoubiquitination transport ER to Golgi vesicle-mediated transport mitotic metaphase plate congression integrin-mediated signaling pathway gastrulation negative regulation of cyclin-dependent protein serine/threonine kinase by cyclin degradation Wnt signaling pathway vesicle-mediated transport cell migration protein ubiquitination stem cell division positive regulation of cytokinesis negative regulation of Rho protein signal transduction embryonic cleavage protein ubiquitination involved in ubiquitin-dependent protein catabolic process stress fiber assembly proteasome-mediated ubiquitin-dependent protein catabolic process fibroblast apoptotic process positive regulation of mitotic metaphase/anaphase transition COPII vesicle coating liver morphogenesis G1/S transition of mitotic cell cycle MAPK cascade cell cycle arrest positive regulation of cell proliferation cell projection organization negative regulation of canonical Wnt signaling pathway intrinsic apoptotic signaling pathway biological_process
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