Proteintech

CUL4A Polyclonal Antibody

CUL4A Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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CUL4A Antibody (14851-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue slide using (Product # 14851-1-AP) CUL4A-Specific antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Product Details

14851-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 20 publications 20 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.3 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody is specifically against CUL4A,but not CUL4B. CUL4A has 2 isoforms with MW 88 kDa (759 aa) and 77 kDa (659 aa), and 14851-1-AP recognizes both.

Target Information

CUL4A (Cullin 4A) is the ubiquitin ligase component of a multimeric complex involved in the degradation of DNA damage-response proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Genetic Modulation of BET1L Confers Colorectal Cancer Susceptibility by Reducing miRNA Binding and m6A Modification.

Cancer research

Li S,Du M,Xu K,Ben S,Zhu T,Guo M,Xin J,Zhu L,Gu D,Zhang Z,Wang M

Wed Jul 05 00:00:00 UTC 2023

Discovery of Potent and Selective 2-(Benzylthio)pyrimidine-based DCN1-UBC12 Inhibitors for Anticardiac Fibrotic Effects.

Journal of medicinal chemistry

He ZX,An Q,Wei B,Zhou WJ,Wei BF,Gong YP,Zhang X,Gao G,Dong GJ,Huo JL,Zhang XH,Yang FF,Liu HM,Ma LY,Zhao W

Thu Jan 13 00:00:00 UTC 2022

Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy.

Signal transduction and targeted therapy

Meng Y,Qiu L,Zeng X,Hu X,Zhang Y,Wan X,Mao X,Wu J,Xu Y,Xiong Q,Chen Z,Zhang B,Han J

Fri Dec 09 00:00:00 UTC 2022

Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy.

Signal transduction and targeted therapy

Meng Y,Qiu L,Zeng X,Hu X,Zhang Y,Wan X,Mao X,Wu J,Xu Y,Xiong Q,Chen Z,Zhang B,Han J

Fri Dec 09 00:00:00 UTC 2022

AMPK induces degradation of the transcriptional repressor PROX1 impairing branched amino acid metabolism and tumourigenesis.

Nature communications

Wang Y,Luo M,Wang F,Tong Y,Li L,Shu Y,Qiao K,Zhang L,Yan G,Liu J,Ji H,Xie Y,Zhang Y,Gao WQ,Liu Y

Thu Nov 24 00:00:00 UTC 2022

MOF negatively regulates estrogen receptor α signaling via CUL4B-mediated protein degradation in breast cancer.

Frontiers in oncology

Zhang X,Yang Y,Li D,Wu Z,Liu H,Zhao Z,Zhu H,Xie F,Li X

Tue Oct 11 00:00:00 UTC 2022

Evaluation of HZX-960, a novel DCN1-UBC12 interaction inhibitor, as a potential antifibrotic compound for liver fibrosis.

Biochemistry and cell biology = Biochimie et biologie cellulaire

Zhou W,Dong G,Gao G,He Z,Xu J,Aziz S,Ma L,Zhao W

Mon Aug 01 00:00:00 UTC 2022

DDB1 binds histone reader BRWD3 to activate the transcriptional cascade in adipogenesis and promote onset of obesity.

Cell reports

Wang X,Wang HY,Hu GS,Tang WS,Weng L,Zhang Y,Guo H,Yao SS,Liu SY,Zhang GL,Han Y,Liu M,Zhang XD,Cen X,Shen HF,Xiao N,Liu CQ,Wang HR,Huang J,Liu W,Li P,Zhao TJ

Tue Jun 22 00:00:00 UTC 2021

Negative regulation of AMPK signaling by high glucose via E3 ubiquitin ligase MG53.

Molecular cell

Jiang P,Ren L,Zhi L,Yu Z,Lv F,Xu F,Peng W,Bai X,Cheng K,Quan L,Zhang X,Wang X,Zhang Y,Yang D,Hu X,Xiao RP

Thu Feb 04 00:00:00 UTC 2021

The Mediator subunit MED20 organizes the early adipogenic complex to promote development of adipose tissues and diet-induced obesity.

Cell reports

Tang WS,Weng L,Wang X,Liu CQ,Hu GS,Yin ST,Tao Y,Hong NN,Guo H,Liu W,Wang HR,Zhao TJ

Tue Jul 06 00:00:00 UTC 2021

The E3 ubiquitin ligase Cul4b promotes CD4+ T cell expansion by aiding the repair of damaged DNA.

PLoS biology

Dar AA,Sawada K,Dybas JM,Moser EK,Lewis EL,Park E,Fazelinia H,Spruce LA,Ding H,Seeholzer SH,Oliver PM

Mon Feb 01 00:00:00 UTC 2021

Cardioprotective effect of MLN4924 on ameliorating autophagic flux impairment in myocardial ischemia-reperfusion injury by Sirt1.

Redox biology

Zhang J,Cui J,Zhao F,Yang L,Xu X,Shi Y,Wei B

Fri Oct 01 00:00:00 UTC 2021

Effect of CUL4A on the metastatic potential of lung adenocarcinoma to the bone.

Oncology reports

Cao PP,Chen WJ,Pang HL,Shen WW,Xue P,Duan L,Xu YX,Liu LL,Zhang HL

Sat Feb 01 00:00:00 UTC 2020

Functional impacts of the ubiquitin-proteasome system on DNA damage recognition in global genome nucleotide excision repair.

Scientific reports

Sakai W,Yuasa-Sunagawa M,Kusakabe M,Kishimoto A,Matsui T,Kaneko Y,Akagi JI,Huyghe N,Ikura M,Ikura T,Hanaoka F,Yokoi M,Sugasawa K

Thu Nov 12 00:00:00 UTC 2020

Cul4 E3 ubiquitin ligase regulates ovarian cancer drug resistance by targeting the antiapoptotic protein BIRC3.

Cell death & disease

Hu X,Meng Y,Xu L,Qiu L,Wei M,Su D,Qi X,Wang Z,Yang S,Liu C,Han J

Mon Feb 04 00:00:00 UTC 2019

Potent 5-Cyano-6-phenyl-pyrimidin-Based Derivatives Targeting DCN1-UBE2M Interaction.

Journal of medicinal chemistry

Zhou W,Ma L,Ding L,Guo Q,He Z,Yang J,Qiao H,Li L,Yang J,Yu S,Zhao L,Wang S,Liu HM,Suo Z,Zhao W

Thu Jun 13 00:00:00 UTC 2019

Cul4a as a New Interaction Protein of PARP1 Inhibits Oxidative Stress-Induced H9c2 Cell Apoptosis.

Oxidative medicine and cellular longevity

Ye N,Zhang N,Zhang Y,Qian H,Wu B,Sun Y

Thu Jan 16 00:00:00 UTC 2020

Cul4-Ddb1 ubiquitin ligases facilitate DNA replication-coupled sister chromatid cohesion through regulation of cohesin acetyltransferase Esco2.

PLoS genetics

Sun H,Zhang J,Xin S,Jiang M,Zhang J,Li Z,Cao Q,Lou H

Fri Feb 01 00:00:00 UTC 2019

Amyloid Precursor Protein (APP) May Act as a Substrate and a Recognition Unit for CRL4CRBN and Stub1 E3 Ligases Facilitating Ubiquitination of Proteins Involved in Presynaptic Functions and Neurodegeneration.

The Journal of biological chemistry

Del Prete D,Rice RC,Rajadhyaksha AM,D'Adamio L

Fri Aug 12 00:00:00 UTC 2016

The Journal of biological chemistry

Del Prete D,Rice RC,Rajadhyaksha AM,D'Adamio L

Fri Aug 12 00:00:00 UTC 2016

AMBRA1 interplay with cullin E3 ubiquitin ligases regulates autophagy dynamics.

Developmental cell

Antonioli M,Albiero F,Nazio F,Vescovo T,Perdomo AB,Corazzari M,Marsella C,Piselli P,Gretzmeier C,Dengjel J,Cecconi F,Piacentini M,Fimia GM

Mon Dec 22 00:00:00 UTC 2014

AMBRA1 interplay with cullin E3 ubiquitin ligases regulates autophagy dynamics.

Developmental cell

Antonioli M,Albiero F,Nazio F,Vescovo T,Perdomo AB,Corazzari M,Marsella C,Piselli P,Gretzmeier C,Dengjel J,Cecconi F,Piacentini M,Fimia GM

Mon Dec 22 00:00:00 UTC 2014

CSN6 drives carcinogenesis by positively regulating Myc stability.

Nature communications

Chen J,Shin JH,Zhao R,Phan L,Wang H,Xue Y,Post SM,Ho Choi H,Chen JS,Wang E,Zhou Z,Tseng C,Gully C,Velazquez-Torres G,Fuentes-Mattei E,Yeung G,Qiao Y,Chou PC,Su CH,Hsieh YC,Hsu SL,Ohshiro K,Shaikenov T,Wang H,Yeung SC,Lee MH

Fri Nov 14 00:00:00 UTC 2014

CSN6 drives carcinogenesis by positively regulating Myc stability.

Nature communications

Fri Nov 14 00:00:00 UTC 2014

COP9 signalosome subunit 8 is essential for peripheral T cell homeostasis and antigen receptor-induced entry into the cell cycle from quiescence.

Nature immunology

Menon S,Chi H,Zhang H,Deng XW,Flavell RA,Wei N

Thu Nov 01 00:00:00 UTC 2007

COP9 signalosome subunit 8 is essential for peripheral T cell homeostasis and antigen receptor-induced entry into the cell cycle from quiescence.

Nature immunology

Menon S,Chi H,Zhang H,Deng XW,Flavell RA,Wei N

Thu Nov 01 00:00:00 UTC 2007

Bioinformatics

Protein Aliases: CUL 4A; CUL-4A; Cul4a protein; Cullin-4A; MGC36573; MGC64071; OTTHUMP00000018753

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Gene Aliases: 2810470J21Rik; AW495282; CUL4A; RGD1563853

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UniProt ID: (Human) Q13619, (Mouse) Q3TCH7

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Entrez Gene ID: (Human) 8451, (Mouse) 99375, (Rat) 361181

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Function(s)

protein binding ubiquitin protein ligase binding ubiquitin protein ligase activity

Process(es)

G1/S transition of mitotic cell cycle nucleotide-excision repair, DNA damage recognition nucleotide-excision repair, DNA duplex unwinding in utero embryonic development transcription-coupled nucleotide-excision repair nucleotide-excision repair, preincision complex stabilization nucleotide-excision repair, preincision complex assembly nucleotide-excision repair, DNA incision, 3'-to lesion nucleotide-excision repair, DNA incision, 5'-to lesion cellular response to DNA damage stimulus cell cycle arrest positive regulation of cell proliferation negative regulation of cell proliferation viral process hemopoiesis negative regulation of granulocyte differentiation nucleotide-excision repair, DNA incision somatic stem cell population maintenance DNA damage response, detection of DNA damage protein ubiquitination involved in ubiquitin-dependent protein catabolic process proteasome-mediated ubiquitin-dependent protein catabolic process regulation of protein metabolic process global genome nucleotide-excision repair intrinsic apoptotic signaling pathway positive regulation of G1/S transition of mitotic cell cycle regulation of DNA damage checkpoint regulation of nucleotide-excision repair DNA repair ubiquitin-dependent protein catabolic process positive regulation of protein catabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CUL4A Polyclonal Antibody

CUL4A Antibody (14851-1-AP) in IHC (P)

Product Details

14851-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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