Invitrogen

CX3CL1 (Fractalkine) Polyclonal Antibody, eBioscience™

CX3CL1 (Fractalkine) Antibody in Western Blot (WB)

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CX3CL1 (Fractalkine) Antibody (14-7986-81) in WB

Western blot was performed using Anti-CX3CL1 (Fractalkine) Polyclonal Antibody, eBioscience™ (Product # 14-7986-81) and a 85 kDa band corresponding to soluble cleaved form of CX3CL1 (Fractalkine) was observed across tested cell line. Conditioned media (20 µg lysate) of HUVEC (Lane 1), HUVEC treated with TNF-alpha, 10 ng/mL for 12 hrs (Lane 2) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Pr... View More

CX3CL1 (Fractalkine) Antibody in Immunocytochemistry (ICC/IF)

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Product Details

14-7986-81

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:5,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:500

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Immunoprecipitation (IP)

1:300-1:800

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_468501

Product Specific Information

Description: The rabbit polyclonal antibody reacts with recombinant and natural mouse, rat, and human fractalkine (CX3CL1); the antibody was generated using E. coli-expressed chemotactic domain of human CX3CL1 as an immunogen. CX3CL1, also known as neurotactin or fractalkine, is a membrane-bound CX3C chemokine. The mature protein is part of a 397-amino acid precursor and consists of a chemokine domain of 76 amino acids, a mucin stalk of 241 amino acids, a putative transmembrane domain of 18 amino acids, and an intracellular tail of 37 amino acids. Within the chemokine domain, the first two cysteine residues are separated by 3 amino acids (CX3C). CX3CL1 message is found in high abundance in the brain, kidney, lung and heart tissues. CX3CL1 is chemotactic for monocytes and other leukocytes including NK cells and may play a role in brain inflammation.

Applications Reported: This polyclonal has been reported for use in immunoblotting (WB), immunoprecipitation, and immunohistochemistry. Cross-reactivity to CX3CL1 of other species has not been determined.

Applications Tested: To achieve best results, titrate antibody by dilution for each application as follows:

Western Blot 1:1000-1:5000

Immunoprecipitation 1:300-1:800

Immunohistochemistry 1:100-1:500.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Chemokines are a family of proteins associated with the trafficking of leukocytes in immune surveillance and inflammatory cell recruitment. They are classified based on the positions of key cysteine residues. CX3CL1 is a CX3C chemokine known to induce adhesion and migration of leukocytes mediated by a membrane-bound and soluble form respectively. Recent experiments have shown that CX3CL1 can suppress the production of nitrous oxide, interleukin-6, and TNF-a in activated microglia and neuronal cells, suggesting that it may act as an intrinsic inhibitor against neurotoxicity by activated microglia. Its receptor, CX3CR1, also functions as a co-receptor for HIV-1 and HIV-2 envelope fusion and virus infection, which can be inhibited by CX3CL1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Fractalkine's dual role in inflammation and hard tissue formation in cultured human dental pulp cells.

Biomedical research (Tokyo, Japan)

Gomyo-Furuya N,Kamio N,Watanabe T,Hayama T,Fukai J,Kuramochi K,Nakanishi K,Watanabe A,Okabe T,Matsushima K

14-7986-81 was used in Western Blot to suggest a dual role for fractalkine in promoting pulp inflammation via COX-2 production and contributing to pulp hard tissue formation by stimulating the expression of hard tissue formation markers.

Thu Nov 30 00:00:00 UTC 2023

iRHOM2 regulates inflammation and endothelial barrier permeability via CX3CL1.

Experimental and therapeutic medicine

Yan H,Wu J,Yan H

14-7986-81 was used in Immunoprecipitation to determine the role of inactive rhomboid-like protein 2 (iRHOM2) in ALI in lipopolysaccharide (LPS)-induced pulmonary microvascular endothelial cell model.

Sat Jul 01 00:00:00 UTC 2023

Fuzheng Huayu Recipe Prevented and Treated CCl4-Induced Mice Liver Fibrosis through Regulating Polarization and Chemotaxis of Intrahepatic Macrophages via CCL2 and CX3CL1.

Evidence-based complementary and alternative medicine : eCAM

Zhang M,Liu HL,Huang K,Peng Y,Tao YY,Zhao CQ,Hu XD,Liu CH

14-7986 was used in Western Blotting to explore the antifibrosis mechanism of FZHY on regulating the recruitment and polarization of intrahepatic macrophages.

Mon Apr 18 00:00:00 UTC 2022

Fuzheng Huayu Recipe Prevented and Treated CCl4-Induced Mice Liver Fibrosis through Regulating Polarization and Chemotaxis of Intrahepatic Macrophages via CCL2 and CX3CL1.

Evidence-based complementary and alternative medicine : eCAM

Zhang M,Liu HL,Huang K,Peng Y,Tao YY,Zhao CQ,Hu XD,Liu CH

14-7986 was used in Western Blotting to explore the antifibrosis mechanism of FZHY on regulating the recruitment and polarization of intrahepatic macrophages.

Mon Apr 18 00:00:00 UTC 2022

Fractalkine shedding is mediated by p38 and the ADAM10 protease under pro-inflammatory conditions in human astrocytes.

Journal of neuroinflammation

O'Sullivan SA,Gasparini F,Mir AK,Dev KK

14-7986 was used in Immunocytochemistry to investigate the regulation of the CX3CL1 ligand in astrocytes.

Mon Aug 22 00:00:00 UTC 2016

MicroRNA-181b regulates NF-κB-mediated vascular inflammation.

The Journal of clinical investigation

Sun X,Icli B,Wara AK,Belkin N,He S,Kobzik L,Hunninghake GM,Vera MP,Blackwell TS,Baron RM,Feinberg MW

Published figure using CX3CL1 (Fractalkine) polyclonal antibody (Product # 14-7986-81) in Western Blot

Fri Jun 01 00:00:00 UTC 2012

Dietary Aloe Improves Insulin Sensitivity via the Suppression of Obesity-induced Inflammation in Obese Mice.

Immune network

Shin E,Shim KS,Kong H,Lee S,Shin S,Kwon J,Jo TH,Park YI,Lee CK,Kim K

14-7986 was used in Western Blotting to investigate whether the aloe component could reduce obesity-induced inflammation and the occurrence of metabolic disorders.

Tue Feb 01 00:00:00 UTC 2011

Fractalkine is expressed in the human ovary and increases progesterone biosynthesis in human luteinised granulosa cells.

Reproductive biology and endocrinology : RB&E

Huang S,Zhao P,Yang L,Chen Y,Yan J,Duan E,Qiao J

14-7986 was used in Immunohistochemistry to provide the first detailed report of fractalkine and CX3CR1 expression and function in the human ovary.

Thu Jun 30 00:00:00 UTC 2011

Differential gene expression profiling of large and small retinal ganglion cells.

Journal of neuroscience methods

Ivanov D,Dvoriantchikova G,Barakat DJ,Nathanson L,Shestopalov VI

14-7986 was used in Immunohistochemistry-agarose to showcase a novel method for the comparative transcriptomic analysis of adult retinal neuronal sub-populations.

Mon Sep 15 00:00:00 UTC 2008

T cell costimulation by fractalkine-expressing synoviocytes in rheumatoid arthritis.

Arthritis and rheumatism

Sawai H,Park YW,Roberson J,Imai T,Goronzy JJ,Weyand CM

Sun May 01 00:00:00 UTC 2005

Bioinformatics

Protein Aliases: ABCD-3; C-X3-C motif chemokine 1; chemokine (C-X3-C motif) ligand 1; CX3C membrane-anchored chemokine; FK; Fractalkine; Neurotactin; small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin); small inducible cytokine subfamily D (Cys-X3-Cys), member-1; small inducible cytokine subfamily D, 1; Small-inducible cytokine D1

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Gene Aliases: A-152E5.2; AB030188; ABCD-3; Acc1; AI848747; C3Xkine; CX3C; CX3CL1; CXC3; CXC3C; D8Bwg0439e; FKN; fractalkine; neurotactin; NTN; NTT; SCYD1

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UniProt ID: (Human) P78423, (Rat) O55145, (Mouse) O35188

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Entrez Gene ID: (Human) 6376, (Rat) 89808, (Mouse) 20312

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Function(s)

receptor binding integrin binding protein binding chemokine activity CX3C chemokine receptor binding CCR chemokine receptor binding cytokine activity

Process(es)

Performance Guarantee

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CX3CL1 (Fractalkine) Polyclonal Antibody, eBioscience™

CX3CL1 (Fractalkine) Antibody (14-7986-81) in WB

Product Details

14-7986-81

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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