Invitrogen

CX3CR1 Monoclonal Antibody (2A9-1), Biotin, eBioscience™

3 Published Figures

5 References

View all (30) CX3CR1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (3)

CX3CR1 Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 4

CX3CR1 Antibody (13-6099-82) in Flow

Staining of normal human peripheral blood cells with 0.125 µg of Rat IgG2b Isotype Control Biotin (Product # 13-4031-82) (left) or 0.125 µg of Anti-Human CX3CR1 Biotin (right) followed by Streptavidin PE (Product # 12-4317-87). Samples were subsequently stained intracellularly with Anti-Human Perforin FITC (Product # 11-9994-42). Cells were gated on CD16+CD56+ using Anti-Human CD16 eFluor® 450 (Product # 48-0168-42) and Anti-Human CD56 APC.

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Product Details

13-6099-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.25 µg/test

View 5 publications 5 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

2A9-1

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_1963592

Product Specific Information

Description: This 2A9-1 monoclonal antibody reacts with human CX3CR1, which is the receptor for fractalkine, a transmembrane chemokine of the CX3C family. CX3CR1 is a seven transmembrane G protein-coupled receptor expressed on CD16+ NK cells, T cells (e.g. CD8+, CD4+, and gamma/delta), and monocytes. In non-immune cells, CX3CR1 has been found on osteoclast precursors and microglia. Little to no CX3CR1 surface expression can be detected on B cells and granulocytes. Together, fractalkine and its receptor mediate cell-cell adhesion and chemotaxis of NK cells, T cells, and monocytes. The expression of CX3CR1 has also been correlated with high levels of intracellular perforin and granzyme B.

Applications Reported: This 2A9-1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 2A9-1 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CX3CR1 is one of the chemokine receptors that are required as coreceptors for HIV infection. The genes encoding human, mouse, and rat CX3CR1 were cloned and designated V28 and CMKBRL1, CX3CR1, and RBS11, respectively. The encoded seven transmembrane protein was recently identified as the receptor for a novel transmembrane molecule, fractalkine, and renamed CX3CR1. Recently, CX3CR1 was found to serve as a coreceptor for HIV-1 and HIV-2 envelope fusion and virus infection, which can be inhibited by fractokine. CX3CR1 mediates leukocyte migration an d adhesion. CX3CR1 is expressed in a variety of human tissues and cell lines.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Distinct gene expression patterns for CD14++ and CD16++ monocytes in preeclampsia.

Scientific reports

Vishnyakova P,Kuznetsova M,Poltavets A,Fomina M,Kiseleva V,Muminova K,Potapova A,Khodzhaeva Z,Pyregov A,Trofimov D,Elchaninov A,Sukhikh G,Fatkhudinov T

Published figure using CX3CR1 monoclonal antibody (Product # 13-6099-82) in Flow Cytometry

Wed Sep 14 00:00:00 UTC 2022

Microglia innately develop within cerebral organoids.

Nature communications

Ormel PR,Vieira de Sá R,van Bodegraven EJ,Karst H,Harschnitz O,Sneeboer MAM,Johansen LE,van Dijk RE,Scheefhals N,Berdenis van Berlekom A,Ribes Martínez E,Kling S,MacGillavry HD,van den Berg LH,Kahn RS,Hol EM,de Witte LD,Pasterkamp RJ

Published figure using CX3CR1 monoclonal antibody (Product # 13-6099-82) in Flow Cytometry

Tue Oct 09 00:00:00 UTC 2018

Modulation of Hematopoietic Lineage Specification Impacts TREM2 Expression in Microglia-Like Cells Derived From Human Stem Cells.

ASN neuro

Amos PJ,Fung S,Case A,Kifelew J,Osnis L,Smith CL,Green K,Naydenov A,Aloi M,Hubbard JJ,Ramakrishnan A,Garden GA,Jayadev S

Published figure using CX3CR1 monoclonal antibody (Product # 13-6099-82) in Flow Cytometry

Fri Dec 08 00:00:00 UTC 2017

Pivotal Role for CD16+ Monocytes in Immune Surveillance of the Central Nervous System.

Journal of immunology (Baltimore, Md. : 1950)

Waschbisch A,Schröder S,Schraudner D,Sammet L,Weksler B,Melms A,Pfeifenbring S,Stadelmann C,Schwab S,Linker RA

13609982 was used in flow cytometry to analyze the frequency and phenotype of monocyte subpopulations in peripheral blood, cerebrospinal fluid, and brain biopsy material derived from multiple sclerosis patients

Mon Feb 15 00:00:00 UTC 2016

The CD14+CD16+ inflammatory monocyte subset displays increased mitochondrial activity and effector function during acute Plasmodium vivax malaria.

PLoS pathogens

Antonelli LR,Leoratti FM,Costa PA,Rocha BC,Diniz SQ,Tada MS,Pereira DB,Teixeira-Carvalho A,Golenbock DT,Gonçalves R,Gazzinelli RT

13-6099 was used in Flow cytometry/Cell sorting to study inflammatory monocyte subsets displaying increased mitochondrial activity and effector function during malaria.

Mon Sep 01 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: Beta chemokine receptor-like 1; C-X3-C CKR-1; chemokine (C-C) receptor-like 1; chemokine (C-X3-C motif) receptor 1; chemokine (C-X3-C) receptor 1; CMK-BRL-1; CMK-BRL1; Cx3c; CX3C chemokine receptor 1; Fractalkine receptor; G protein-coupled receptor 13; G-protein coupled receptor 13; Scyd1; V28

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Gene Aliases: CCRL1; CMKBRL1; CMKDR1; CX3CR1; GPR13; GPRV28; V28

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UniProt ID: (Human) P49238

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Entrez Gene ID: (Human) 1524

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Function(s)

chemokine receptor activity protein binding C-X3-C chemokine receptor activity C-X3-C chemokine binding

Process(es)

positive regulation of neuroblast proliferation microglial cell activation involved in immune response negative regulation of chronic inflammatory response to non-antigenic stimulus chemotaxis cellular defense response cell adhesion G-protein coupled receptor signaling pathway memory response to wounding viral process negative regulation of angiogenesis cerebral cortex cell migration negative regulation of cell migration positive regulation of angiogenesis macrophage chemotaxis chemokine-mediated signaling pathway cellular response to lipopolysaccharide cellular response to transforming growth factor beta stimulus negative regulation of long-term synaptic potentiation negative regulation of extrinsic apoptotic signaling pathway in absence of ligand

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CX3CR1 Monoclonal Antibody (2A9-1), Biotin, eBioscience™

CX3CR1 Antibody (13-6099-82) in Flow

Product Details

13-6099-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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