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Invitrogen
CXCL1 Polyclonal Antibody
FIGURE: 1 / 2
CXCL1 Antibody (PA5-46932) in Neu
Neutralization assay of recombinant human CXCL1/GROa chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by recombinant human CXCL1/GROa (0.01 µg/mL) is neutralized (green line) by increasing concentrations of human CXCL1/GROa Antigen Affinity-purified Polyclonal Antibody (Product # PA5-46932). The ND50 is typically 0.03-0.15 µg/mL.
Product Details
PA5-46932
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived recombinant human CXCL1/GRO alpha/KC/CINC-1 Ala35-Asn107
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2609589
Product Specific Information
In direct ELISAs, less than 50% cross-reactivity with recombinant human (rh) GRO beta and rhGRO gamma is observed, less than 25% cross-reactivity with recombinant mouse (rm) MIP-2 is observed, and less than 15% cross-reactivity with recombinant rat (rr) CINC-1, rrCINC-2 alpha, rrCINC-2 beta, rmKC, and rhIL-8 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Target Information
CXCL1, CXCL2, and CXCL3, also known respectively as GRO alpha, GRO beta (MIP-2 alpha) and GRO gamma (MIP-2 beta), are members of the CXC subfamily of chemokines. Mature CXCL1/2/3 proteins bind with high affinity to the IL-8 receptor type B and are potent neutrophil attractants and activators. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 1; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha); CXC; CXC motif chemokine; CXCL; CXCL-1; fibroblast secretory protein; GRO a; GRO α; GRO-alpha(1-73); GRO1 oncogene (melanoma growth stimulating activity, alpha); GRO1 oncogene (melanoma growth-stimulating activity); GROalpha; Growth-regulated alpha protein; GROα; melanoma growth stimulating activity, alpha; Melanoma growth stimulatory activity; melanoma growth stimulatory activity alpha; MGSA; MGSA alpha; NAP-3; NAP3; Neutrophil-activating protein 3
Gene Aliases: CXCL1; FSP; GRO; GRO1; GROA; MGSA; MGSA-a; NAP-3; SCYB1
UniProt ID: (Human) P09341
Entrez Gene ID: (Human) 2919
chemotaxis
inflammatory response
immune response
signal transduction
G-protein coupled receptor signaling pathway
nervous system development
cell proliferation
negative regulation of cell proliferation
actin cytoskeleton organization
response to lipopolysaccharide
intracellular signal transduction
positive regulation of catalytic activity
cell chemotaxis
chemokine-mediated signaling pathway
positive regulation of neutrophil chemotaxis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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