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Invitrogen
CXCL10 Recombinant Rabbit Monoclonal Antibody (10H11L3)
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FIGURE: 1 / 4
CXCL10 Antibody (701225) in ICC/IF
Immunofluorescence analysis of C-X-C motif chemokine 10 was performed using 70% confluent log phase HT-29 cells and HT-29 cells treated with 50 ng/mL IFN gamma for 14 hr followed by 1x PTI treatment for 4h. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CXCL10 Recombinant Rabbit Monoclonal Antibody (10H11L3) (Product # 701225) at 1:200 in 0.1% BSA, incubated at 4 degree celsius overnig... View More
Product Details
701225
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
10H11L3
Immunogen
proprietary
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2532429
Product Specific Information
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
IP-10 (interferon-gamma induced protein 10 kD; in mouse, also called cytokine responsive gene 2, Crg-2, or mob-1) is a highly inducible, primary response gene that belongs to the CXC chemokine superfamily. It was first cloned in 1985. The biological functions of IP-10 are still unclear. Like Mig, IP-10 has no activity on neutrophils. Its functions include stimulation of monocytes, natural killer and T-cell migration, regulation of T-cell and bone marrow progenitor maturation, modulation of adhesion molecule expression as well as inhibition of angiogenesis. Mig and IP-10 share the same receptor, CXCR3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 10 kDa interferon gamma-induced protein; C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 10; C7; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 10; CXC; CXC motif chemokine; CXCL; gamma IP10; Gamma-IP10; H-IP-10; interferon activated gene 10; Interferon-gamma induced protein CRG-2; interferon-gamma-induced protein CRG-2; interferon-inducible cytokine IP-10; M-IP-10; protein 10 from interferon (gamma)-induced cell line; small inducible cytokine B subfamily (Cys-X-Cys), member 10; small inducible cytokine subfamily B (Cys-X-Cys), member 10; Small-inducible cytokine B10
Gene Aliases: C7; CRG-2; Crg2; CXCL10; gIP-10; IFI10; INP10; IP-10; IP10; mob-1; SCYB10
UniProt ID: (Human) P02778, (Mouse) P17515
Entrez Gene ID: (Human) 3627, (Mouse) 15945
chemotaxis
inflammatory response
immune response
signal transduction
cell surface receptor signaling pathway
G-protein coupled receptor signaling pathway
cell-cell signaling
muscle organ development
blood circulation
positive regulation of cell proliferation
response to cold
response to gamma radiation
T cell chemotaxis
regulation of T cell chemotaxis
response to auditory stimulus
negative regulation of angiogenesis
positive regulation of cAMP metabolic process
response to vitamin D
cellular response to heat
endothelial cell activation
regulation of cell proliferation
positive regulation of cAMP-mediated signaling
negative regulation of myoblast differentiation
regulation of protein kinase activity
positive regulation of transcription from RNA polymerase II promoter
positive regulation of release of sequestered calcium ion into cytosol
defense response to virus
chemokine-mediated signaling pathway
cellular response to lipopolysaccharide
positive regulation of monocyte chemotaxis
regulation of endothelial tube morphogenesis
negative regulation of myoblast fusion
positive regulation of T cell migration
positive regulation of leukocyte chemotaxis
positive regulation of cell migration
response to lipopolysaccharide
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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