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Invitrogen
CXCL12 Recombinant Rabbit Monoclonal Antibody (JJ09-20)
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FIGURE: 1 / 3
CXCL12 Antibody (MA5-42461) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of of SDF1 in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with CXCL12 Recombinant Monoclonal Antibody (Product # MA5-42461) at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (blue... View More
Product Details
MA5-42461
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JJ09-20
Immunogen
Recombinant full length protein of human SDF1
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2911602
Product Specific Information
Positive Control: SH-SY5Y, SW480, 293.
Subcellular Location: Secreted.
Target Information
CXCL12 is a stromal cell-derived alpha chemokine member of the intercrine family. This gene product and its receptor CXCR4 can activate lymphocytes and have been implicated in the metastasis of some cancers such as breast cancer. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AI174028; C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 12; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 12; CXC; CXC motif chemokine; CXCL; hIRH; hSDF-1; Intercrine reduced in hepatomas; IRH; PBSF; Pre-B cell growth-stimulating factor; SDF-1; SDF-1a; SDF-1b; Stromal cell-derived factor 1; stromal cell-derived factor-1 alpha; TLSF-a; TLSF-b; Tlsfa; Tlsfb
Gene Aliases: CXCL12; IRH; PBSF; SCYB12; SDF1; SDF1A; SDF1B; TLSF; TPAR1
UniProt ID: (Human) P48061
Entrez Gene ID: (Human) 6387
response to hypoxia
neuron migration
positive regulation of endothelial cell proliferation
cellular calcium ion homeostasis
chemotaxis
immune response
cell adhesion
signal transduction
G-protein coupled receptor signaling pathway
axon guidance
blood circulation
regulation of actin polymerization or depolymerization
adult locomotory behavior
response to radiation
response to heat
response to mechanical stimulus
response to virus
telencephalon cell migration
organ regeneration
positive regulation of dopamine secretion
response to peptide hormone
positive regulation of cell adhesion
positive regulation of axon extension involved in axon guidance
positive chemotaxis
induction of positive chemotaxis
cell chemotaxis
chemokine-mediated signaling pathway
positive regulation of monocyte chemotaxis
positive regulation of calcium ion import
negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage
negative regulation of leukocyte tethering or rolling
negative regulation of leukocyte apoptotic process
positive regulation of T cell migration
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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