Invitrogen

CXCL12 alpha (SDF-1 alpha) Polyclonal Antibody, eBioscience™

CXCL12 alpha (SDF-1 alpha) Antibody in Immunohistochemistry (IHC)

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CXCL12 alpha (SDF-1 alpha) Antibody (14-7992-81) in IHC

Journal of cellular and molecular medicine 2020 - Tfh cells are an important source of CXCL12 in mice with schistosomiasis japonica. WT and icosl -/- mice were infected with 12 cercariae of Schistosoma japonicum per mouse, and sacrificed at 8 wk post-infection. (A, B) Spleens, mesenteric LN and livers from normal and infected mice were harvested, and cells were stained with CD3-percpcy5.5, CD4-PE-Cy7, CXCR5-FITC, PD-1-PE and CXCL12-APC antibodies. CXCL12 +... View More

CXCL12 alpha (SDF-1 alpha) Antibody in Immunocytochemistry, Immunohistochemistry (Frozen) (ICC/IF, IHC (F))

CXCL12 alpha (SDF-1 alpha) Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P))

CXCL12 alpha (SDF-1 alpha) Antibody in Western Blot, ELISA (WB, ELISA)

CXCL12 alpha (SDF-1 alpha) Antibody in Flow Cytometry (Flow)

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Product Details

14-7992-81

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:500

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:500

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

View 1 publication 1 publication

ELISA (ELISA)

View 1 publication 1 publication

Immunoprecipitation (IP)

1:300-1:500

Product Specifications

Species Reactivity

Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_468513

Product Specific Information

Description: The polyclonal antibody reacts with both mouse and rat SDF-1 alpha. It was was generated using E. coli-expressed N terminal amio acids 20-89 of mouse SDF-1 alpha as an immunogen. SDF-1 alpha (stromal cell-derived factor-1 alpha), also known as pre-B-cell growth-stimulating factor (PBSF), is a 70-amino acid CXC chemokine originally cloned from a bone marrow stromal cell line. Targeted deletion of the SDF-1 gene resulted in defects of B-cell lymphopoiesis and bone marrow myelopoiesis. SDF-1 has been shown to be chemotactic for lymphocytes. In addition, SDF-1 was recently reported to be a ligand for CXCR4 (LESTR/fusin), a co-receptor for HIV-1 entry into T cells. SDF-1 binding to CXCR4 inhibits HIV-1 entry.

Applications Reported: This polyclonal has been reported for use in immunoblotting (WB), immunoprecipitation, and immunohistochemistry. Cross-reactivity to SDF-1 alpha of other species has not been determined.

Applications Tested: To achieve best results, titrate antibody by dilution for each application as follows:

Western Blot 1:1000

Immunoprecipitation 1:300-500

Immunohistochemistry 1:100-500.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CXCL12 is a stromal cell-derived alpha chemokine member of the intercrine family. This gene product and its receptor CXCR4 can activate lymphocytes and have been implicated in the metastasis of some cancers such as breast cancer. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

CXCL12-CXCR4 Interplay Facilitates Palatal Osteogenesis in Mice.

Frontiers in cell and developmental biology

Verheijen N,Suttorp CM,van Rheden REM,Regan RF,Helmich MPAC,Kuijpers-Jagtman AM,Wagener FADTG

14-7992 was used in Immunohistochemistry to provide novel evidence that CXCL12-CXCR4 interplay facilitates palatal osteogenesis and palatal fusion in mice.

Mon Sep 28 00:00:00 UTC 2020

CXCL12-CXCR4 Interplay Facilitates Palatal Osteogenesis in Mice.

Frontiers in cell and developmental biology

Verheijen N,Suttorp CM,van Rheden REM,Regan RF,Helmich MPAC,Kuijpers-Jagtman AM,Wagener FADTG

14-7992 was used in Immunohistochemistry to provide novel evidence that CXCL12-CXCR4 interplay facilitates palatal osteogenesis and palatal fusion in mice.

Mon Sep 28 00:00:00 UTC 2020

CXCL12-CXCR4 Interplay Facilitates Palatal Osteogenesis in Mice.

Frontiers in cell and developmental biology

Verheijen N,Suttorp CM,van Rheden REM,Regan RF,Helmich MPAC,Kuijpers-Jagtman AM,Wagener FADTG

14-7992 was used in Immunohistochemistry to provide novel evidence that CXCL12-CXCR4 interplay facilitates palatal osteogenesis and palatal fusion in mice.

Mon Sep 28 00:00:00 UTC 2020

Follicular helper T cells recruit eosinophils into host liver by producing CXCL12 during Schistosoma japonicum infection.

Journal of cellular and molecular medicine

Chen X,Xu Z,Wei C,Yang X,Xu L,Zhou S,Zhu J,Su C

14-7992-81 was used in Immunohistochemistry to uncover a previously unappreciated role of Tfh cells in promotion of the development of liver granuloma in schistosomiasis, making Tfh-CXCL12-eosinophil axis a potential target for intervention of schistosomiasis.

Sat Feb 01 00:00:00 UTC 2020

Follicular helper T cells recruit eosinophils into host liver by producing CXCL12 during Schistosoma japonicum infection.

Journal of cellular and molecular medicine

Chen X,Xu Z,Wei C,Yang X,Xu L,Zhou S,Zhu J,Su C

Sat Feb 01 00:00:00 UTC 2020

Role of the high-affinity leukotriene B4 receptor signaling in fibrosis after unilateral ureteral obstruction in mice.

PloS one

Kamata M,Amano H,Ito Y,Fujita T,Otaka F,Hosono K,Kamata K,Takeuchi Y,Yokomizo T,Shimizu T,Majima M

14-7992 was used in Immunohistochemistry-immunofluorescence to examine the role of the high-affinity leukotriene B4 receptor, BLT1, during the development of kidney fibrosis induced by unilateral ureteral obstruction.

Mon Dec 02 00:00:00 UTC 2019

Role of the high-affinity leukotriene B4 receptor signaling in fibrosis after unilateral ureteral obstruction in mice.

PloS one

Kamata M,Amano H,Ito Y,Fujita T,Otaka F,Hosono K,Kamata K,Takeuchi Y,Yokomizo T,Shimizu T,Majima M

Mon Dec 02 00:00:00 UTC 2019

Role of the high-affinity leukotriene B4 receptor signaling in fibrosis after unilateral ureteral obstruction in mice.

PloS one

Kamata M,Amano H,Ito Y,Fujita T,Otaka F,Hosono K,Kamata K,Takeuchi Y,Yokomizo T,Shimizu T,Majima M

Mon Dec 02 00:00:00 UTC 2019

The Chemokine Receptor CXCR4 Mediates Recruitment of CD11c+ Conventional Dendritic Cells Into the Inflamed Murine Cornea.

Investigative ophthalmology & visual science

Lopez MJ,Seyed-Razavi Y,Jamali A,Harris DL,Hamrah P

14-7992 was used in Immunohistochemistry-immunoflourescence to investigate the presence of CXCR4 and its ligand, CXCL12, in the murine cornea, and its role in conventional dendritic cell migration during corneal inflammation.

Thu Nov 01 00:00:00 UTC 2018

The Chemokine Receptor CXCR4 Mediates Recruitment of CD11c+ Conventional Dendritic Cells Into the Inflamed Murine Cornea.

Investigative ophthalmology & visual science

Lopez MJ,Seyed-Razavi Y,Jamali A,Harris DL,Hamrah P

Thu Nov 01 00:00:00 UTC 2018

Biliary Epithelial Cells Are Not the Predominant Source of Hepatic CXCL12.

The American journal of pathology

Saiman Y,Sugiyama T,Simchoni N,Spirli C,Bansal MB

Wed Jul 01 00:00:00 UTC 2015

Biliary Epithelial Cells Are Not the Predominant Source of Hepatic CXCL12.

The American journal of pathology

Saiman Y,Sugiyama T,Simchoni N,Spirli C,Bansal MB

Wed Jul 01 00:00:00 UTC 2015

Biliary Epithelial Cells Are Not the Predominant Source of Hepatic CXCL12.

The American journal of pathology

Saiman Y,Sugiyama T,Simchoni N,Spirli C,Bansal MB

Wed Jul 01 00:00:00 UTC 2015

Chronic morphine and HIV-1 Tat promote differential central nervous system trafficking of CD3+ and Ly6C+ immune cells in a murine Streptococcus pneumoniae infection model.

Journal of neuroinflammation

Dutta R,Roy S

14-7992 was used in Western Blotting to explore the role of systemic infection with Streptococcus pneumoniae in the recruitment of peripheral leukocytes into the central nervous system.

Sat Jun 20 00:00:00 UTC 2015

Targeting CXCR7/ACKR3 as a therapeutic strategy to promote remyelination in the adult central nervous system.

The Journal of experimental medicine

Williams JL,Patel JR,Daniels BP,Klein RS

14-7992 was used in Western Blotting to identify a role for CXCR7 in OPC maturation during remyelination.

Mon May 05 00:00:00 UTC 2014

miR-27b represses migration of mouse MSCs to burned margins and prolongs wound repair through silencing SDF-1a.

PloS one

Lü MH,Hu CJ,Chen L,Peng X,Chen J,Hu JY,Teng M,Liang GP

14-7992 was used in Western Blotting to gain insight into the molecular mechanisms involved in regulating stromal cell-derived factor-1α.

Mon Feb 24 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: 12-O-tetradecanoylphorbol 13-acetate repressed protein 1; C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 12; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1); CXC; CXC motif chemokine; CXCL; H-SDF-1-alpha; PBSF; Pre-B cell growth-stimulating factor; pre-B-cell growth-stimulating factor; SDF 1 a; SDF 1 alpha; SDF 1α; SDF-1; SDF-1 gamma; SDF1 alpha; SDF1α; Stromal cell-derived factor 1; stromal cell-derived factor-1 alpha; stromal cell-derived factor-1 gamma; Thymic lymphoma cell-stimulating factor; TLSF; TPAR1

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Gene Aliases: Cxcl12; Pbsf; Scyb12; Sdf1; Tlsf; Tpar1

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UniProt ID: (Mouse) P40224

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Entrez Gene ID: (Rat) 24772, (Mouse) 20315

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Function(s)

chemokine activity chemokine receptor binding CXCR chemokine receptor binding cytokine activity growth factor activity

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CXCL12 alpha (SDF-1 alpha) Polyclonal Antibody, eBioscience™

CXCL12 alpha (SDF-1 alpha) Antibody (14-7992-81) in IHC

Product Details

14-7992-81

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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