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Invitrogen
CXCL13 Monoclonal Antibody (DS8CX13), APC, eBioscience™
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FIGURE: 1 / 3
CXCL13 Antibody (17-7981-82) in Flow
BALB/c mouse resident peritoneal macrophages were stained with CD11b Monoclonal Antibody, FITC (Product # 11-0112-82) and F4/80 Monoclonal Antibody, eFluor 450 (Product # 48-4801-82). The cells were then fixed and permeabilized using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol, and stained with 0.06 µg of Rat IgG2a kappa Isotype Control, APC (Product # 17-4321-81) (left) or 0.06 µg of CXCL13 Monoclonal Antibody, APC (right). CD11b positive cells were used for analysis.
Product Details
17-7981-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
DS8CX13
Immunogen
Full length CXCL13
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2762702
Product Specific Information
Description: This DS8CX13 monoclonal antibody recognizes mouse CXCL13 also known as BCA-1 and Angie.
Applications Reported: This DS8CX13 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This DS8CX13 antibody has been tested by flow cytometric analysis of resident peritoneal macrophages. This may be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser
Target Information
CXCL13, also known as BCA-1 and Angie, is a small chemokine strongly expressed in the secondary lymphoid organs. It preferentially promotes the migration of B lymphocytes (both B-1 and B-2) to the follicles of the spleen, lymph nodes, and Peyer's patches. It is produced by macrophages, and in mouse the highest constitutive producers include large peritoneal macrophages, Kuppfer cells and lung macrophages (both CD11c+ and CD11c-). The cellular ligand for CXCL13 is CXCR5 also known as BLR-1 (Burkitt’s Lymphoma Receptor 1). CXCR5 is expressed on B cells, Tfh cells and, to a lower degree, some memory T cell populations. CXCL13 plays an important role in the development of chronic lymphocytic leukemia (CCL). CXCL13 is also a plasma biomarker for germinal center activity and generation of broadly neutralizing antibodies against HIV.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B lymphocyte chemoattractant; C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 13; CC motif chemokine; CCmotif chemokine; chem; CXC; CXC chemokine BLC; CXC motif chemokine; CXC motif chemokine 13; CXCL; small inducible cytokine subfamily B (Cys-X-Cys), member 13; Small-inducible cytokine B13
Gene Aliases: 4631412M08Rik; Angie; ANGIE2; BCA-1; BLC; BLR1L; Cxcl13; Scyb13
UniProt ID: (Mouse) O55038
Entrez Gene ID: (Mouse) 55985
lymphocyte chemotaxis across high endothelial venule
positive regulation of leukocyte chemotaxis
chemotaxis
inflammatory response
immune response
cell surface receptor signaling pathway
G-protein coupled receptor signaling pathway
positive regulation of cytosolic calcium ion concentration
cell-cell signaling
positive regulation of T cell chemotaxis
response to lipopolysaccharide
positive regulation of integrin activation
positive regulation of cell-cell adhesion mediated by integrin
B cell chemotaxis
endothelial cell chemotaxis to fibroblast growth factor
B cell chemotaxis across high endothelial venule
regulation of cell proliferation
defense response to bacterium
lymph node development
chemokine-mediated signaling pathway
activation of GTPase activity
positive regulation of receptor activity
negative regulation of endothelial cell chemotaxis to fibroblast growth factor
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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