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CXCL2 Antibody (500-P104BT-25UG) in WB
Western Blot: To detect Human GRO-beta by Western Blot analysis CXCL2 Polyclonal Antibody, Biotin (Product # 500-P104BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human GRO-beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P104BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human GRO-beta (CXCL2)
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929207
Product Specific Information
AA Sequence of recombinant protein: APLATELRCQ CLQTLQGIHL KNIQSVKVKS PGPHCAQTEV IATLKNGQKA CLNPASPMVK KIIEKMLKNG KSN.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human GRO-beta (CXCL2). Anti-Human GRO-beta (CXCL2)-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human GRO-beta (CXCL2) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human GRO-beta (CXCL2) (500-P104) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human GRO-beta (CXCL2).
Western Blot: To detect Human GRO-beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human GRO-beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P104BT-1MG will be provided as 2 x 500 µg
Target Information
Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 2; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 2; CXC; CXC motif chemokine; CXCL; GRO b; gro beta; GRO β; Gro-beta; GRO2 oncogene; GRObeta; Growth-regulated protein beta; GROβ; Macrophage inflammatory protein 2-alpha; melanoma growth stimulatory activity beta; MGSA beta; MIP 2 a; MIP 2 alpha; MIP 2 α; MIP2 a; MIP2 α; MIP2-alpha
Gene Aliases: CINC-2a; CXCL2; GRO2; GROB; MGSA-b; MIP-2a; MIP2; MIP2A; SCYB2
UniProt ID: (Human) P19875
Entrez Gene ID: (Human) 2920
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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