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CXCL6 Antibody (PA5-115276) in ICC/IF
Immunocytochemistry analysis of CXCL6 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115276) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Product Details
PA5-115276
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human CXCL6(Accession P80162), corresponding to amino acid residues R10-P60.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2899912
Product Specific Information
Antibody detects endogenous levels of total GCP2.
Target Information
CXCL6 is a chemotactic for neutrophil granulocytes. It signals through binding and activation of its receptors (CXCR1 and CXCR2). In addition to its chemotactic and angiogenic properties, it has strong antibacterial activity against Gram-positive and Gram-negative bacteria (90-fold-higher when compared to CXCL5 and CXCL7).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 5; C-X-C motif chemokine 6; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 5; chemokine (C-X-C motif) ligand 6 (granulocyte chemotactic protein 2); Chemokine alpha 3; CKA-3; CKA3; CXC; CXC chemokine LIX; CXC motif chemokine; CXCL; Cytokine LIX; GCP-2; Granulocyte chemotactic protein 2; small inducible cytokine subfamily B (Cys-X-Cys), member 6 (granulocyte chemotactic protein 2); Small inducible cytokine subfamily B (Cys-X-Cys), member b; Small-inducible cytokine B5; Small-inducible cytokine B6
Gene Aliases: CKA-3; Cxcl5; CXCL6; GCP-2; GCP2; Scyb5; SCYB6
UniProt ID: (Human) P80162, (Rat) P97885
Entrez Gene ID: (Human) 6372, (Rat) 60665
leukocyte homeostasis
cytokine production
neutrophil mediated immunity
positive regulation of leukocyte chemotaxis
inflammatory response
immune response
G-protein coupled receptor signaling pathway
response to gamma radiation
positive regulation of neuron projection development
midbrain development
organ regeneration
response to lipopolysaccharide
regulation of chemokine production
regulation of cell proliferation
positive regulation of JAK-STAT cascade
cell chemotaxis
chemokine-mediated signaling pathway
regulation of neutrophil mediated killing of gram-negative bacterium
cellular response to lipopolysaccharide
chemotaxis
signal transduction
cell-cell signaling
defense response to bacterium
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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