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CXCL6 Antibody (PA5-46937) in Neu
Neutralization of CXCL6 in BaF3 mouse pro‚B cell line transfected with human CXCR2. Samples were incubated in CXCL6 polyclonal antibody (Product # PA5-46937). Recombinant Human CXCL6/GCP‚2 chemoattracts the BaF3 mouse pro‚B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Human CXCL6/GCP‚2 (80 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human... View More
Product Details
PA5-46937
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived recombinant human CXCL6/GCP-2 Val40-Asn114
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2608254
Product Specific Information
In direct ELISAs and Western blots, less than 10% cross-reactivity with recombinant mouse (rm) MIP-2, rmKC, rmCXCL6, recombinant human (rh) ENA-78, recombinant rat (rr) CINC-2 alpha, rmCRG-2, rhNAP-2 is observed and less than 5% cross-reactivity with rrCINC-2 beta and rhMCP-3 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Endoxin level is <0.20 EU per 1 µg of the antibody by the LAL method.
Target Information
CXCL6 is a chemotactic for neutrophil granulocytes. It signals through binding and activation of its receptors (CXCR1 and CXCR2). In addition to its chemotactic and angiogenic properties, it has strong antibacterial activity against Gram-positive and Gram-negative bacteria (90-fold-higher when compared to CXCL5 and CXCL7).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 6; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 6 (granulocyte chemotactic protein 2); Chemokine alpha 3; CKA-3; CKA3; CXC; CXC motif chemokine; CXCL; GCP-2; Granulocyte chemotactic protein 2; small inducible cytokine subfamily B (Cys-X-Cys), member 6 (granulocyte chemotactic protein 2); Small inducible cytokine subfamily B (Cys-X-Cys), member b; Small-inducible cytokine B6
Gene Aliases: CKA-3; CXCL6; GCP-2; GCP2; SCYB6
UniProt ID: (Human) P80162
Entrez Gene ID: (Human) 6372
neutrophil mediated immunity
positive regulation of leukocyte chemotaxis
chemotaxis
inflammatory response
immune response
signal transduction
G-protein coupled receptor signaling pathway
cell-cell signaling
response to lipopolysaccharide
regulation of cell proliferation
defense response to bacterium
cell chemotaxis
chemokine-mediated signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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