Proteintech

CXCL8/IL8 Polyclonal Antibody

CXCL8/IL8 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 4

CXCL8/IL8 Antibody (27095-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using (Product # 27095-1-AP) (CXCL8/IL8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Product Details

27095-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:1,000

View 13 publications 13 publications

Immunohistochemistry (IHC)

Assay-dependent

View 13 publications 13 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

CXCL8/IL8 Fusion Protein Ag25640 (40-99 aa encoded by BC013615)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.4 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: YSKPFHPKF IKELRVIESG PHCANTEIIV KLSDGRELCL DPKENWVQRV VEKFLKRAEN S (40-99 aa encoded by BC013615)

Target Information

Interleukin 8 (IL-8, CXCL8) is a 72 amino acid pro-inflammatory factor which belongs to the CXC subfamily of chemokines, and are bound by the cell surface receptors IL-8RA and IL-8RB. IL-8 functions as a chemoattractant and potent angiogenic factor. The expression and secretion of IL-8 can be induced by diverse inflammatory stimuli in many cells, including macrophages and endothelial cells. In endothelial cells, IL-8 is present in storage vesicles called Weibel-Palade bodies. IL-8, first isolated from osteosarcoma cells, contains the ELR-motif (N-terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors. Previous nomenclature for IL-8 includes neutrophil activating protein 1 (NAP-1), granulocyte chemotactic protein 1 (GCP-1), monocyte-derived neutrophil-activating peptide (MONAP) and protein 3-10C. IL-8 and ten other members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. Cancer studies have demonstrated a role for IL-8 in the angiogenesis and growth of tumours, and IL-8 is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Development of Neovasculature in Axially Vascularized Calcium Phosphate Cement Scaffolds.

Journal of functional biomaterials

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Tue Feb 14 00:00:00 UTC 2023

Calcipotriol abrogates cancer-associated fibroblast-derived IL-8-mediated oxaliplatin resistance in gastric cancer cells via blocking PI3K/Akt signaling.

Acta pharmacologica Sinica

Zhao ZX,Zhang YQ,Sun H,Chen ZQ,Chang JJ,Wang X,Wang X,Tan C,Ni SJ,Weng WW,Zhang M,Wang L,Huang D,Feng Y,Sheng WQ,Xu MD

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PKCθ Regulates Pituitary Adenoma Bone Invasion by Activating Osteoclast in NF-κB/IL-1β-Dependent Manner.

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Mon Mar 06 00:00:00 UTC 2023

Fusobacterium nucleatum promotes esophageal squamous cell carcinoma progression and chemoresistance by enhancing the secretion of chemotherapy-induced senescence-associated secretory phenotype via activation of DNA damage response pathway.

Gut microbes

Zhang JW,Zhang D,Yin HS,Zhang H,Hong KQ,Yuan JP,Yu BP

Thu Apr 06 00:00:00 UTC 2023

Carboxymethyl chitosan regulates macrophages polarization to inhibit early subconjunctival inflammation in conjunctival injury.

International journal of biological macromolecules

Geng X,Wang Y,Cui H,Li C,Cheng B,Cui B,Liu R,Zhang J,Zhu L,Li J,Shen J,Li Z

Mon Jul 31 00:00:00 UTC 2023

Intervening Effects and Molecular Mechanism of Quercitrin on PCV2-Induced Histone Acetylation, Oxidative Stress and Inflammatory Response in 3D4/2 Cells.

Antioxidants (Basel, Switzerland)

Chen Q,Wei Y,Zhao Y,Xie X,Kuang N,Wei Y,Yu M,Hu T

Wed May 11 00:00:00 UTC 2022

Quercetin Ameliorates Renal Injury and Pyroptosis in Lupus Nephritis through Inhibiting IL-33/ST2 Pathway In Vitro and In Vivo.

Antioxidants (Basel, Switzerland)

Chen HY,Chiang YF,Hong YH,Shieh TM,Huang TC,Ali M,Chang HY,Wang KL,Hsia SM

Sun Nov 13 00:00:00 UTC 2022

The AGEs/RAGE Transduction Signaling Prompts IL-8/CXCR1/2-Mediated Interaction between Cancer-Associated Fibroblasts (CAFs) and Breast Cancer Cells.

Cells

Santolla MF,Talia M,Cirillo F,Scordamaglia D,De Rosis S,Spinelli A,Miglietta AM,Nardo B,Filippelli G,De Francesco EM,Belfiore A,Lappano R,Maggiolini M

Thu Aug 04 00:00:00 UTC 2022

FumDSB can alleviate the inflammatory response induced by fumonisin B₁ in growing pigs.

Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment

Liu Q,Huang L,Cui Z,Qiao B,Li F,Wang C

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FumDSB can alleviate the inflammatory response induced by fumonisin B₁ in growing pigs.

Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment

Liu Q,Huang L,Cui Z,Qiao B,Li F,Wang C

Thu Sep 01 00:00:00 UTC 2022

Loss of miR-637 promotes cancer cell stemness via WASH/IL-8 pathway and serves as a novel prognostic marker in esophageal squamous cell carcinoma.

Biomarker research

Guo M,Lian J,Liu Y,Dong B,He Q,Zhao Q,Zhang H,Qi Y,Zhang Y,Huang L

Thu Nov 03 00:00:00 UTC 2022

Tri-n-butyl phosphate delays tissue repair by dysregulating neutrophil function in zebrafish.

Toxicology and applied pharmacology

Wang Y,Cheng Z,Zhang H,Li S,Pan Y,Zhang W,Huang S,He X,Zou F,Yuan Z,Yan W,Huang H

Mon Aug 15 00:00:00 UTC 2022

Microbial and metabolic profiles of bronchopulmonary dysplasia and therapeutic effects of potential probiotics Limosilactobacillus reuteri and Bifidobacterium bifidum.

Journal of applied microbiology

Li Y,He L,Zhao Q,Bo T

Mon Aug 01 00:00:00 UTC 2022

Blood flow in the anterior humeral circumflex artery reflects synovial inflammation of the shoulder joint in rotator cuff tears.

JSES international

Asano H,Terabayashi N,Kawashima K,Goto A,Watanabe T,Ishihara T,Akiyama H

Fri Jul 01 00:00:00 UTC 2022

The flavonoid procyanidin C1 has senotherapeutic activity and increases lifespan in mice.

Nature metabolism

Xu Q,Fu Q,Li Z,Liu H,Wang Y,Lin X,He R,Zhang X,Ju Z,Campisi J,Kirkland JL,Sun Y

Wed Dec 01 00:00:00 UTC 2021

Fibroblast membrane-camouflaged nanoparticles for inflammation treatment in the early stage.

International journal of oral science

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Tue Nov 16 00:00:00 UTC 2021

Single-Cell Transcriptome Analysis of Chronic Antibody-Mediated Rejection After Renal Transplantation.

Frontiers in immunology

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5-Aminolaevulinic acid photodynamic therapy amplifies intense inflammatory response in the treatment of acne vulgaris via CXCL8.

Experimental dermatology

Zhang L,Yang J,Liu X,Xu D,Shi L,Liu J,Zeng Q,Wang X

Thu Jul 01 00:00:00 UTC 2021

Galectin‑3 facilitates the proliferation and migration of nasopharyngeal carcinoma cells via activation of the ERK1/2 and Akt signaling pathways, and is positively correlated with the inflammatory state of nasopharyngeal carcinoma.

Molecular medicine reports

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Sat May 01 00:00:00 UTC 2021

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Legal medicine (Tokyo, Japan)

Ikeda T,Tani N,Watanabe M,Hirokawa T,Ikeda K,Morioka F,Ishikawa T

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Oncology letters

Li A,Zhang N,Zhao Z,Chen Y,Zhang L

Sat Aug 01 00:00:00 UTC 2020

Overexpression of B7-H4 promotes renal cell carcinoma progression by recruiting tumor-associated neutrophils via upregulation of CXCL8.

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Frontiers in cell and developmental biology

Qi B,Yang C,Zhu Z,Chen H

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Journal of thoracic disease

Kida Y,Ohshimo S,Kyo M,Hosokawa K,Amatya VJ,Takeshima Y,Shime N

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MicroRNA-506 suppresses invasiveness and metastasis of human hepatocellular carcinoma cells by targeting IL8.

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Wang Z,Si M,Yang N,Zhang H,Fu Y,Yan K,Zong Y,Zhu N,Wei Y

Fri Apr 08 00:00:00 UTC 2022

MicroRNA-506 suppresses invasiveness and metastasis of human hepatocellular carcinoma cells by targeting IL8.

American journal of cancer research

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Wed Mar 30 00:00:00 UTC 2022

Antichronic Gastric Ulcer Effect of Zinc-Baicalin Complex on the Acetic Acid-Induced Chronic Gastric Ulcer Rat Model.

Gastroenterology research and practice

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Wed Mar 30 00:00:00 UTC 2022

IL-8 Secreted from M2 Macrophages Promoted Prostate Tumorigenesis via STAT3/MALAT1 Pathway.

International journal of molecular sciences

Zheng T,Ma G,Tang M,Li Z,Xu R

Thu Dec 27 00:00:00 UTC 2018

IL-8 Secreted from M2 Macrophages Promoted Prostate Tumorigenesis via STAT3/MALAT1 Pathway.

International journal of molecular sciences

Zheng T,Ma G,Tang M,Li Z,Xu R

Thu Dec 27 00:00:00 UTC 2018

Bioinformatics

Protein Aliases: alveolar macrophage chemotactic factor I; ANX7; beta endothelial cell-derived neutrophil activating peptide; beta-thromboglobulin-like protein; C-X-C motif chemokine 8; Chemokine (C-X-C motif) ligand 8; Emoctakin; GCP-1; Granulocyte chemotactic protein 1; IL-8; ILN; Interleukin; interleukin 8; Interleukin-8; Interleukin8; lung giant cell carcinoma-derived chemotactic protein; lymphocyte derived neutrophil activating peptide; MDNCF; MONAP; Monocyte-derived neutrophil chemotactic factor; Monocyte-derived neutrophil-activating peptide; NAP-1; neutrophil-activating peptide 1; Neutrophil-activating protein 1; Protein 3-10C; RP11-537A6.8; small inducible cytokine subfamily B, member 8; SNX; SYNEXIN; T-cell chemotactic factor; tumor necrosis factor-induced gene 1

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Gene Aliases: CXCL8; GCP-1; GCP1; IL8; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP-1; NAP1

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UniProt ID: (Human) P10145

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Entrez Gene ID: (Human) 3576

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Function(s)

interleukin-8 receptor binding protein binding chemokine activity

Process(es)

angiogenesis response to molecule of bacterial origin movement of cell or subcellular component chemotaxis inflammatory response immune response cell cycle arrest signal transduction G-protein coupled receptor signaling pathway negative regulation of cell proliferation calcium-mediated signaling regulation of cell adhesion neutrophil chemotaxis receptor internalization response to endoplasmic reticulum stress intracellular signal transduction PERK-mediated unfolded protein response neutrophil activation cellular response to fibroblast growth factor stimulus regulation of single stranded viral RNA replication via double stranded DNA intermediate negative regulation of G-protein coupled receptor protein signaling pathway positive regulation of angiogenesis embryonic digestive tract development induction of positive chemotaxis chemokine-mediated signaling pathway cellular response to lipopolysaccharide cellular response to interleukin-1 cellular response to tumor necrosis factor positive regulation of neutrophil chemotaxis

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CXCL8/IL8 Polyclonal Antibody

CXCL8/IL8 Antibody (27095-1-AP) in IHC (P)

Product Details

27095-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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