Invitrogen

CXCL9 Recombinant Rabbit Monoclonal Antibody (11H1L14)

CXCL9 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 8

CXCL9 Antibody (701117) in IHC (P)

Immunohistochemistry analysis of MIG showing staining in the cytoplasm and nucleus of paraffin-embedded mouse thymus tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room tempe... View More

CXCL9 Antibody in Immunocytochemistry (ICC/IF)

CXCL9 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

CXCL9 Antibody — Modulation of expression of target protein by cell treatment demonstrates antibody specificity. Immunofluorescence analysis of CXCL9 using anti-CXCL9 rabbit monoclonal antibody (Product # 701117) shows increase in expression of CXCL9 in theTHP1 cell upon treatment with IFNgamma in cytoplasm {TM}

Product Details

701117

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-1 µg/mL

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20

Immunocytochemistry (ICC/IF)

2 µg/mL

Dot blot (DB)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

11H1L14

Immunogen

Recombinant protein corresponding to amino acids 23-125 of human monokine induced by Interferon-gamma

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

AB_2532396

Product Specific Information

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

MIG, also known as CXCL9, is a T-cell chemoattractant inducible by gamma interferon that is a member of the CXC chemokine family of cytokines.This gene is mapped to 4q21. It is noted that, although the best-described activities of the chemokines are as chemotactic factors, chemokines also have an effect on T-cell activation, angiogenesis, and HIV infection. While most CXC chemokines are chemotactic for neutrophils, MIG and INP10 are unusual and similar in being CXC chemokines that are chemotactic for lymphocytes and inactive in neutrophils.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Histone deacetylases inhibitor chidamide synergizes with humanized PD1 antibody to enhance T-cell chemokine expression and augment Ifn-γ response in NK-T cell lymphoma.

EBioMedicine

Wen T,Sun G,Jiang W,He X,Shi Y,Ma F,Liu P

701117 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to investigate the combined effect of the humanized PD1 antibody and different HDAC inhibitors in human PD1 gene knock-in mouse models, illustrate the underlying mechanisms, and discover predictive biomarkers.

Sun Jan 01 00:00:00 UTC 2023

Histone deacetylases inhibitor chidamide synergizes with humanized PD1 antibody to enhance T-cell chemokine expression and augment Ifn-γ response in NK-T cell lymphoma.

EBioMedicine

Wen T,Sun G,Jiang W,He X,Shi Y,Ma F,Liu P

Sun Jan 01 00:00:00 UTC 2023

Neuroprotection against ischemic stroke requires a specific class of early responder T cells in mice.

The Journal of clinical investigation

Cai W,Shi L,Zhao J,Xu F,Dufort C,Ye Q,Yang T,Dai X,Lyu J,Jin C,Pu H,Yu F,Hassan S,Sun Z,Zhang W,Hitchens TK,Shi Y,Thomson AW,Leak RK,Hu X,Chen J

701117 was used in Immunohistochemistry to show that these unique CD8+ TRLs serve as early responders to rally defenses against stroke, offering fresh perspectives for clinical translation.

Mon Aug 01 00:00:00 UTC 2022

Senescent hepatocytes enhance natural killer cell activity via the CXCL-10/CXCR3 axis.

Experimental and therapeutic medicine

Zang J,Ye J,Zhang C,Sha M,Gao J

Published figure using CXCL9 recombinant monoclonal antibody (Product # 701117) in Dot blot

Fri Nov 01 00:00:00 UTC 2019

LIF regulates CXCL9 in tumor-associated macrophages and prevents CD8⁺ T cell tumor-infiltration impairing anti-PD1 therapy.

Nature communications

Pascual-García M,Bonfill-Teixidor E,Planas-Rigol E,Rubio-Perez C,Iurlaro R,Arias A,Cuartas I,Sala-Hojman A,Escudero L,Martínez-Ricarte F,Huber-Ruano I,Nuciforo P,Pedrosa L,Marques C,Braña I,Garralda E,Vieito M,Squatrito M,Pineda E,Graus F,Espejo C,Sahuquillo J,Tabernero J,Seoane J

701117 was used in Immunohistochemistry to study the effect of the combination of LIF neutralising antibodies with the inhibition of the PD1 immune checkpoint on tumour progression.

Tue Jun 11 00:00:00 UTC 2019

Bioinformatics

Protein Aliases: C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 9; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 9; CXC; CXC motif chemokine; CXCL; gamma interferon-induced monokine; Gamma-interferon-induced monokine; HuMIG; M119; MIG; monokine induced by gamma interferon; Monokine induced by interferon-gamma; Protein m119; small inducible cytokine B subfamily (Cys-X-Cys), member 9; Small-inducible cytokine B9

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Gene Aliases: BB139920; CMK; crg-10; CXCL9; Humig; MIG; MuMIG; SCYB9

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UniProt ID: (Human) Q07325, (Mouse) P18340

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Entrez Gene ID: (Human) 4283, (Mouse) 17329

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Function(s)

cytokine activity protein binding chemokine activity CXCR3 chemokine receptor binding

Process(es)

positive regulation of leukocyte chemotaxis chemotaxis defense response inflammatory response immune response cellular defense response signal transduction G-protein coupled receptor signaling pathway cell-cell signaling positive regulation of cAMP metabolic process response to lipopolysaccharide regulation of cell proliferation positive regulation of cAMP-mediated signaling positive regulation of myoblast differentiation positive regulation of release of sequestered calcium ion into cytosol defense response to virus cell chemotaxis chemokine-mediated signaling pathway positive regulation of myoblast fusion T cell chemotaxis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CXCL9 Recombinant Rabbit Monoclonal Antibody (11H1L14)

CXCL9 Antibody (701117) in IHC (P)

Product Details

701117

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Dot blot (DB)

Species Reactivity

Published species

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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