Proteintech

CYB5R3 Polyclonal Antibody

CYB5R3 Antibody in Immunocytochemistry (ICC/IF)

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CYB5R3 Antibody (10894-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells, using CYB5R3 antibody 10894-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

CYB5R3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CYB5R3 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

10894-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 16 publications 16 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 6 publications 6 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Not applicable (N/A)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Pig, Rat

Published species

Hamster, Human, Mouse, Pig, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

CYB5R3 Fusion Protein Ag1339 (1-301 aa encoded by BC004821)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.18 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MGAQLSTLG HMVLFPVWFL YSLLMKLFQR STPAITLESP DIKYPLRLID REIISHDTRR FRFALPSPQH ILGLPVGQHI YLSARIDGNL VVRPYTPISS DDDKGFVDLV IKVYFKDTHP KFPAGGKMSQ YLESMQIGDT IEFRGPSGLL VYQGKGKFAI RPDKKSNPII RTVKSVGMIA GGTGITPMLQ VIRAIMKDPD DHTVCHLLFA NQTEKDILLR PELEELRNKH SARFKLWYTL DRAPEAWDYG QGFVNEEMIR DHLPPPEEEP LVLMCGPPPM IQYACLPNLD HVGHPTERCF VF (1-301 aa encoded by BC004821)

Target Information

CYB5R3 (NADH-cytochrome b5, reductase 3, diaphorase 1) is a 301 amino acid protein encoded by the human gene CYB5R3. CYB5R3 belongs to the flavoprotein pyridine nucleotide cytochrome reductase family and has two naturally occuring isoforms. Isoform 1 is anchored to the cytoplasmic side of the endoplasmic reticulum membrane and mitochondrion outer membrane, while isoform 2 is the soluble form found in erythrocytes. CYB5R3 is involved in the desaturation and elongation of fatty acids, cholesterol biosynthesis,drug metabolism and, in erythrocytes, methemoglobin reduction.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Oxidized soluble guanylyl cyclase causes erectile dysfunction in alcoholic mice.

British journal of pharmacology

Olivencia MA,Gil de Biedma-Elduayen L,Giménez-Gómez P,Barreira B,Fernández A,Angulo J,Colado MI,O'Shea E,Perez-Vizcaino F

Fri Sep 01 00:00:00 UTC 2023

CYB5R3 in type II alveolar epithelial cells protects against lung fibrosis by suppressing TGF-β1 signaling.

JCI insight

Bueno M,Calyeca J,Khaliullin T,Miller MP,Alvarez D,Rosas L,Brands J,Baker C,Nasser A,Shulkowski S,Mathien A,Uzoukwu N,Sembrat J,Mays BG,Fiedler K,Hahn SA,Salvatore SR,Schopfer FJ,Rojas M,Sandner P,Straub AC,Mora AL

Wed Mar 08 00:00:00 UTC 2023

CYB5R3 in type II alveolar epithelial cells protects against lung fibrosis by suppressing TGF-β1 signaling.

JCI insight

Wed Mar 08 00:00:00 UTC 2023

CYB5R3 in type II alveolar epithelial cells protects against lung fibrosis by suppressing TGF-β1 signaling.

JCI insight

Wed Mar 08 00:00:00 UTC 2023

CYB5R3 in type II alveolar epithelial cells protects against lung fibrosis by suppressing TGF-β1 signaling.

JCI insight

Wed Mar 08 00:00:00 UTC 2023

Cyb5r3-based mechanism and reversal of secondary failure to sulfonylurea in diabetes.

Science translational medicine

Watanabe H,Du W,Son J,Sui L,Asahara SI,Kurland IJ,Kuo T,Kitamoto T,Miyachi Y,de Cabo R,Accili D

Wed Feb 01 00:00:00 UTC 2023

CYB5R3 overexpression preserves skeletal muscle mitochondria and autophagic signaling in aged transgenic mice.

GeroScience

López-Bellón S,Rodríguez-López S,González-Reyes JA,Burón MI,de Cabo R,Villalba JM

Mon Aug 01 00:00:00 UTC 2022

Cigarette smoke induces pulmonary arterial dysfunction through an imbalance in the redox status of the soluble guanylyl cyclase.

Free radical biology & medicine

Sevilla-Montero J,Munar-Rubert O,Pino-Fadón J,Aguilar-Latorre C,Villegas-Esguevillas M,Climent B,Agrò M,Choya-Foces C,Martínez-Ruiz A,Balsa E,Muñoz-Calleja C,Gómez-Punter RM,Vázquez-Espinosa E,Cogolludo A,Calzada MJ

Sun Nov 20 00:00:00 UTC 2022

The UFM1 system regulates ER-phagy through the ufmylation of CYB5R3.

Nature communications

Ishimura R,El-Gowily AH,Noshiro D,Komatsu-Hirota S,Ono Y,Shindo M,Hatta T,Abe M,Uemura T,Lee-Okada HC,Mohamed TM,Yokomizo T,Ueno T,Sakimura K,Natsume T,Sorimachi H,Inada T,Waguri S,Noda NN,Komatsu M

Wed Dec 21 00:00:00 UTC 2022

Role of cytoglobin in cigarette smoke constituent-induced loss of nitric oxide bioavailability in vascular smooth muscle cells.

Nitric oxide : biology and chemistry

Mahgoup EM,Khaleel SA,El-Mahdy MA,Abd-Allah AR,Zweier JL

Tue Feb 01 00:00:00 UTC 2022

Smooth muscle cell CYB5R3 preserves cardiac and vascular function under chronic hypoxic stress.

Journal of molecular and cellular cardiology

Durgin BG,Wood KC,Hahn SA,McMahon B,Baust JJ,Straub AC

Sat Jan 01 00:00:00 UTC 2022

Identification and Validation of a Nine-Gene Amino Acid Metabolism-Related Risk Signature in HCC.

Frontiers in cell and developmental biology

Zhao Y,Zhang J,Wang S,Jiang Q,Xu K

Sat Sep 25 00:00:00 UTC 2021

Mitochondrial adaptations in liver and skeletal muscle to pro-longevity nutritional and genetic interventions: the crosstalk between calorie restriction and CYB5R3 overexpression in transgenic mice.

GeroScience

Rodríguez-López S,López-Bellón S,González-Reyes JA,Burón MI,de Cabo R,Villalba JM

Mon Jun 01 00:00:00 UTC 2020

GGPP depletion initiates metaflammation through disequilibrating CYB5R3-dependent eicosanoid metabolism.

The Journal of biological chemistry

Wei L,Zheng YY,Sun J,Wang P,Tao T,Li Y,Chen X,Sang Y,Chong D,Zhao W,Zhou Y,Wang Y,Jiang Z,Qiu T,Li CJ,Zhu MS,Zhang X

Fri Nov 20 00:00:00 UTC 2020

Smooth muscle cytochrome b5 reductase 3 deficiency accelerates pulmonary hypertension development in sickle cell mice.

Blood advances

Wood KC,Durgin BG,Schmidt HM,Hahn SA,Baust JJ,Bachman T,Vitturi DA,Ghosh S,Ofori-Acquah SF,Mora AL,Gladwin MT,Straub AC

Tue Dec 10 00:00:00 UTC 2019

Loss of smooth muscle CYB5R3 amplifies angiotensin II-induced hypertension by increasing sGC heme oxidation.

JCI insight

Durgin BG,Hahn SA,Schmidt HM,Miller MP,Hafeez N,Mathar I,Freitag D,Sandner P,Straub AC

Thu Oct 03 00:00:00 UTC 2019

Conditional deletion of smooth muscle Cullin-3 causes severe progressive hypertension.

JCI insight

Agbor LN,Nair AR,Wu J,Lu KT,Davis DR,Keen HL,Quelle FW,McCormick JA,Singer JD,Sigmund CD

Tue Jun 11 00:00:00 UTC 2019

Dia1-dependent adhesions are required by epithelial tissues to initiate invasion.

The Journal of cell biology

Fessenden TB,Beckham Y,Perez-Neut M,Ramirez-San Juan G,Chourasia AH,Macleod KF,Oakes PW,Gardel ML

Mon Apr 02 00:00:00 UTC 2018

High expression of cytochrome b 5 reductase isoform 3/cytochrome b 5 system in the cerebellum and pyramidal neurons of adult rat brain.

Brain structure & function

Samhan-Arias AK,López-Sánchez C,Marques-da-Silva D,Lagoa R,Garcia-Lopez V,García-Martínez V,Gutierrez-Merino C

Sun May 01 00:00:00 UTC 2016

High expression of cytochrome b 5 reductase isoform 3/cytochrome b 5 system in the cerebellum and pyramidal neurons of adult rat brain.

Brain structure & function

Samhan-Arias AK,López-Sánchez C,Marques-da-Silva D,Lagoa R,Garcia-Lopez V,García-Martínez V,Gutierrez-Merino C

Sun May 01 00:00:00 UTC 2016

Structure Guided Chemical Modifications of Propylthiouracil Reveal Novel Small Molecule Inhibitors of Cytochrome b5 Reductase 3 That Increase Nitric Oxide Bioavailability.

The Journal of biological chemistry

Rahaman MM,Reinders FG,Koes D,Nguyen AT,Mutchler SM,Sparacino-Watkins C,Alvarez RA,Miller MP,Cheng D,Chen BB,Jackson EK,Camacho CJ,Straub AC

Fri Jul 03 00:00:00 UTC 2015

Purified NADH-cytochrome b5 reductase is a novel superoxide anion source inhibited by apocynin: sensitivity to nitric oxide and peroxynitrite.

Free radical biology & medicine

Samhan-Arias AK,Gutierrez-Merino C

Fri Aug 01 00:00:00 UTC 2014

Purified NADH-cytochrome b5 reductase is a novel superoxide anion source inhibited by apocynin: sensitivity to nitric oxide and peroxynitrite.

Free radical biology & medicine

Samhan-Arias AK,Gutierrez-Merino C

Fri Aug 01 00:00:00 UTC 2014

Caveolin-rich lipid rafts of the plasma membrane of mature cerebellar granule neurons are microcompartments for calcium/reactive oxygen and nitrogen species cross-talk signaling.

Cell calcium

Marques-da-Silva D,Gutierrez-Merino C

Fri Aug 01 00:00:00 UTC 2014

Caveolin-rich lipid rafts of the plasma membrane of mature cerebellar granule neurons are microcompartments for calcium/reactive oxygen and nitrogen species cross-talk signaling.

Cell calcium

Marques-da-Silva D,Gutierrez-Merino C

Fri Aug 01 00:00:00 UTC 2014

Stimulation and clustering of cytochrome b5 reductase in caveolin-rich lipid microdomains is an early event in oxidative stress-mediated apoptosis of cerebellar granule neurons.

Journal of proteomics

Samhan-Arias AK,Marques-da-Silva D,Yanamala N,Gutierrez-Merino C

Wed Jun 06 00:00:00 UTC 2012

Stimulation and clustering of cytochrome b5 reductase in caveolin-rich lipid microdomains is an early event in oxidative stress-mediated apoptosis of cerebellar granule neurons.

Journal of proteomics

Samhan-Arias AK,Marques-da-Silva D,Yanamala N,Gutierrez-Merino C

Wed Jun 06 00:00:00 UTC 2012

Stimulation and clustering of cytochrome b5 reductase in caveolin-rich lipid microdomains is an early event in oxidative stress-mediated apoptosis of cerebellar granule neurons.

Journal of proteomics

Samhan-Arias AK,Marques-da-Silva D,Yanamala N,Gutierrez-Merino C

Wed Jun 06 00:00:00 UTC 2012

Stimulation and clustering of cytochrome b5 reductase in caveolin-rich lipid microdomains is an early event in oxidative stress-mediated apoptosis of cerebellar granule neurons.

Journal of proteomics

Samhan-Arias AK,Marques-da-Silva D,Yanamala N,Gutierrez-Merino C

Wed Jun 06 00:00:00 UTC 2012

L-type calcium channels and cytochrome b5 reductase are components of protein complexes tightly associated with lipid rafts microdomains of the neuronal plasma membrane.

Journal of proteomics

Marques-da-Silva D,Samhan-Arias AK,Tiago T,Gutierrez-Merino C

Wed Jun 16 00:00:00 UTC 2010

L-type calcium channels and cytochrome b5 reductase are components of protein complexes tightly associated with lipid rafts microdomains of the neuronal plasma membrane.

Journal of proteomics

Marques-da-Silva D,Samhan-Arias AK,Tiago T,Gutierrez-Merino C

Wed Jun 16 00:00:00 UTC 2010

L-type calcium channels and cytochrome b5 reductase are components of protein complexes tightly associated with lipid rafts microdomains of the neuronal plasma membrane.

Journal of proteomics

Marques-da-Silva D,Samhan-Arias AK,Tiago T,Gutierrez-Merino C

Wed Jun 16 00:00:00 UTC 2010

L-type calcium channels and cytochrome b5 reductase are components of protein complexes tightly associated with lipid rafts microdomains of the neuronal plasma membrane.

Journal of proteomics

Marques-da-Silva D,Samhan-Arias AK,Tiago T,Gutierrez-Merino C

Wed Jun 16 00:00:00 UTC 2010

Clustering of plasma membrane-bound cytochrome b5 reductase within 'lipid raft' microdomains of the neuronal plasma membrane.

Molecular and cellular neurosciences

Samhan-Arias AK,Garcia-Bereguiain MA,Martin-Romero FJ,Gutierrez-Merino C

Thu Jan 01 00:00:00 UTC 2009

Clustering of plasma membrane-bound cytochrome b5 reductase within 'lipid raft' microdomains of the neuronal plasma membrane.

Molecular and cellular neurosciences

Samhan-Arias AK,Garcia-Bereguiain MA,Martin-Romero FJ,Gutierrez-Merino C

Thu Jan 01 00:00:00 UTC 2009

Clustering of plasma membrane-bound cytochrome b5 reductase within 'lipid raft' microdomains of the neuronal plasma membrane.

Molecular and cellular neurosciences

Samhan-Arias AK,Garcia-Bereguiain MA,Martin-Romero FJ,Gutierrez-Merino C

Thu Jan 01 00:00:00 UTC 2009

Clustering of plasma membrane-bound cytochrome b5 reductase within 'lipid raft' microdomains of the neuronal plasma membrane.

Molecular and cellular neurosciences

Samhan-Arias AK,Garcia-Bereguiain MA,Martin-Romero FJ,Gutierrez-Merino C

Thu Jan 01 00:00:00 UTC 2009

Neuronal expression and subcellular localization of cholesterol 24-hydroxylase in the mouse brain.

The Journal of comparative neurology