Invitrogen

CYLD Monoclonal Antibody (733)

CYLD Antibody in Immunocytochemistry (ICC/IF)

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CYLD Antibody (43-7700) in ICC/IF

Immunofluorescence analysis of CYLD was done on 70% confluent log phase SHSY5Y cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with CYLD Mouse Monoclonal Antibody (Product # 43-7700) at 1 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution... View More

CYLD Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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CYLD Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. Hep G2 cells were transfected with CYLD siRNA and decrease in signal intensity was observed in Western Blot application using Anti-CYLD Monoclonal Antibody (733) (Product # 43-7700). {KD}

Product Details

43-7700

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:50

Immunocytochemistry (ICC/IF)

1 µg/mL

Product Specifications

Species Reactivity

Bovine, Horse, Human, Mouse, Non-human primate, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

733

Immunogen

Recombinant protein derived from the N-terminus of human CYLD protein

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533539

Target Information

CYLD is a 956 aa, cytoplasmic, deubiquitinating enzyme belonging to the ubiquitin carboxy-terminal hydrolases (UCH) family of proteins with three cytoskeletal-associated protein-glycine-conserved (CAP-GLY) domains, a proline rich region, a SH3 binding domain and a sequence homology to catalytic domain of UCH. CYLD is identified as a tumor suppressor protein affecting the JNK signaling pathway. CYLD is a negative regulator of TRAF2 and NF-kappa-B signaling pathway and is also known to have receptor-dependent role in regulating the I-kappa-B kinase pathway. It also has a deubiquitinating activity that is directed towards non-Lys-48-linked polyubiquitin chains and TRAP1 is a novel substrate for deubiquitination. Mutated CYLD is known to be associated with cylindromatosis, multiple familial trichoepithelioma, and Brooke-Spiegler syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

EML2-S constitutes a new class of proteins that recognizes and regulates the dynamics of tyrosinated microtubules.

Current biology : CB

Hotta T,McAlear TS,Yue Y,Higaki T,Haynes SE,Nesvizhskii AI,Sept D,Verhey KJ,Bechstedt S,Ohi R

43-7700 was used in Western Blot to increase microtubule stability.

Mon Sep 26 00:00:00 UTC 2022

Direct activation of RIP3/MLKL-dependent necrosis by herpes simplex virus 1 (HSV-1) protein ICP6 triggers host antiviral defense.

Proceedings of the National Academy of Sciences of the United States of America

Wang X,Li Y,Liu S,Yu X,Li L,Shi C,He W,Li J,Xu L,Hu Z,Yu L,Yang Z,Chen Q,Ge L,Zhang Z,Zhou B,Jiang X,Chen S,He S

Published figure using CYLD monoclonal antibody (Product # 43-7700) in Western Blot

Tue Oct 28 00:00:00 UTC 2014

Necroptosis mediates TNF-induced toxicity of hippocampal neurons.

BioMed research international

Liu S,Wang X,Li Y,Xu L,Yu X,Ge L,Li J,Zhu Y,He S

Published figure using CYLD monoclonal antibody (Product # 43-7700) in Western Blot

Mon Mar 30 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: cylindromatosis (turban tumor syndrome); Deubiquitinating enzyme CYLD; FLJ20180; FLJ31664; FLJ78684; probable ubiquitin carboxyl-terminal hydrolase CYLD; retinitis pigmentosa 1 homolog; Ubiquitin carboxyl-terminal hydrolase CYLD; ubiquitin specific peptidase like 2; Ubiquitin thioesterase CYLD; ubiquitin thiolesterase CYLD; Ubiquitin-specific-processing protease CYLD

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Gene Aliases: 2010013M14Rik; 2900009M21Rik; BOS_17130; BRSS; C130039D01Rik; CDMT; CYLD; CYLD1; CYLDI; EAC; HSPC057; KIAA0849; LRRGT00003; MFT; MFT1; mKIAA0849; Rp1; Rp1h; SBS; TEM; USPL2

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UniProt ID: (Human) Q9NQC7, (Bovine) Q1RMU2, (Rat) Q66H62, (Mouse) Q80TQ2

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Entrez Gene ID: (Human) 1540, (Bovine) 536421, (Rat) 312937, (Mouse) 74256

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Function(s)

thiol-dependent ubiquitin-specific protease activity protein binding zinc ion binding protein kinase binding Lys63-specific deubiquitinase activity proline-rich region binding obsolete ubiquitin thiolesterase activity molecular_function structural constituent of ribosome peptidase activity cysteine-type peptidase activity hydrolase activity thiol-dependent ubiquitinyl hydrolase activity metal ion binding

Process(es)

ubiquitin-dependent protein catabolic process cell cycle regulation of mitotic cell cycle regulation of tumor necrosis factor-mediated signaling pathway Wnt signaling pathway negative regulation of NF-kappaB transcription factor activity negative regulation of type I interferon production negative regulation of NF-kappaB import into nucleus negative regulation of T cell differentiation necroptotic process nucleotide-binding oligomerization domain containing signaling pathway regulation of microtubule cytoskeleton organization protein K63-linked deubiquitination negative regulation of canonical Wnt signaling pathway ripoptosome assembly involved in necroptotic process regulation of cilium assembly positive regulation of extrinsic apoptotic signaling pathway regulation of intrinsic apoptotic signaling pathway cytoplasmic translation biological_process protein deubiquitination proteolysis apoptotic process

Performance Guarantee

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CYLD Monoclonal Antibody (733)

CYLD Antibody (43-7700) in ICC/IF

Product Details

43-7700

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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