Invitrogen

CYP26A1 Polyclonal Antibody

5 Published Figures

View all (16) CYP26A1 antibodies

Questions & Answers

Questions & Answers

Antibody Testing Data (3)
Published Figures (5)

CYP26A1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CYP26A1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 8

CYP26A1 Antibody (PA5-24602) in IHC (P)

Immunohistochemistry analysis of CYP26A1 in formalin fixed and paraffin embedded brain tissue. Samples were incubated with CYP26A1 polyclonal antibody (Product # PA5-24602) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.

CYP26A1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CYP26A1 Antibody in Western Blot (WB)

CYP26A1 Antibody in Flow Cytometry (Flow)

CYP26A1 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

CYP26A1 Antibody in Western Blot (WB)

CYP26A1 Antibody in Western Blot, Flow Cytometry (WB, Flow)

CYP26A1 Antibody in Flow Cytometry (Flow)

CYP26A1 Antibody in Flow Cytometry (Flow)

Product Image Gallery

CYP26A1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of CYP26A1 in formalin fixed and paraffin embedded brain tissue. Samples were incubated with CYP26A1 polyclonal antibody (Product # PA5-24602) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.

CYP26A1 Antibody in Western Blot (WB) — Western blot analysis of CYP26A1 in mouse NIH-3T3 cell lysates. Samples were incubated with CYP26A1 polyclonal antibody (Product # PA5-24602). Lysates: 35 µg/lane. CYP26A1 (arrow).

CYP26A1 Antibody in Flow Cytometry (Flow) — Flow cytometry of CYP26A1 in NIH-3T3 cells (right histogram). Samples were incubated with CYP26A1 polyclonal antibody (Product # PA5-24602) followed by FITC-conjugated goat-anti-rabbit secondary antibody. Negative control cell (left histogram).

CYP26A1 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF) — 1 FIGURE Expression of Cyp26a1 in mouse uterine tissue and uterine NK cells during the peri-implantation period. (A) qPCR to detect the expression of Cyp26a1 on gd4-gd7 uterine tissue. (B) Western blotting to detect the expression of Cyp26a1 on gd4-gd7 uterine tissue. (C) qPCR to detect the expression of Cyp26a1 in CD45 + CD3 - CD122 + NK cells isolated from the uterus and spleen on gd5. (D) qPCR to detect the expression of Cyp26a1 in CD45 + CD3 - CD122 + NK cells isolated from the uterus on gd4-gd7. (E) Representative graph of flow cytometric assays to detect CD45 + CD3 - CD122 + Cyp26a1 + NK cells in the uterus on gd4-gd7, the marker molecules were as follows: CD3-PE, CD45-PerCP-Cy5.5, CD122-APC and Cyp26a1-Alexa Fluor 488, The cells under gate CD45 + CD3 - shown in the figure. (F) Percentages of Cyp26a1 + NK cells in gated CD45 + CD3 - CD122 + NK and CD122 + NK cells in gated CD45 + CD3 - cells in the uterus on gd4-gd7. (G) Upper picture: immunofluorescence detection of Cyp26a1 expression in CD45 + CD3 - CD122 + NK cells isolated from the uterus on gd5 (63 x objective); lower picture: confocal photography of Cyp26a1 + NK cells and Cyp26a1 - NK cells isolated from the uterus on gd5 (20 x and 63 x objective). Data were means +- SEM from three biological replicates, *represents P < .05, **represents P < .01 (two-tailed unpaired Student's t test) (A and C). Data were representative of two (A, B and C) or three (E, F and G) independent experiments

CYP26A1 Antibody in Western Blot (WB) — Figure 2 Differential expression of Cyp26a1 in M1 and M2 macrophages. (A) qPCR analysis of Cyp26a1 in M1 and M2 of GD6 uterine macrophages (D6 U Mphi), Raw264.7 and iBMDM (n=3). (B) qPCR analysis of Cyp26a1 in M0, M1 and M2 macrophages of Raw264.7 at different induction time (LPS (100 ng/mL) + IFN-gamma(20 ng/mL); 2 h, 4 h, 8 h, 12 h and 24 h; n=3). (C) Protein expression of CYP26A1 in M0, M1, M2 macrophages of Raw264.7 at different induction time (12 h and 24 h) was analyzed by Western blot. GAPDH was used as loading control. (D) qPCR analysis of Cyp26a1 in M1-like and M2-like uterine macrophages of GD5 and GD6 (n=3). Error bars represent means +- SEM; two-tailed unpaired t-test, ns, not significant, *P < 0.05, **P < 0.01, or ***P < 0.001.

CYP26A1 Antibody in Western Blot, Flow Cytometry (WB, Flow) — FIGURE 1 Expression of Cyp26a1 in mouse uterine tissue and uterine NK cells during the peri-implantation period. (A) qPCR to detect the expression of Cyp26a1 on gd4-gd7 uterine tissue. (B) Western blotting to detect the expression of Cyp26a1 on gd4-gd7 uterine tissue. (C) qPCR to detect the expression of Cyp26a1 in CD45 + CD3 - CD122 + NK cells isolated from the uterus and spleen on gd5. (D) qPCR to detect the expression of Cyp26a1 in CD45 + CD3 - CD122 + NK cells isolated from the uterus on gd4-gd7. (E) Representative graph of flow cytometric assays to detect CD45 + CD3 - CD122 + Cyp26a1 + NK cells in the uterus on gd4-gd7, the marker molecules were as follows: CD3-PE, CD45-PerCP-Cy5.5, CD122-APC and Cyp26a1-Alexa Fluor 488, The cells under gate CD45 + CD3 - shown in the figure. (F) Percentages of Cyp26a1 + NK cells in gated CD45 + CD3 - CD122 + NK and CD122 + NK cells in gated CD45 + CD3 - cells in the uterus on gd4-gd7. (G) Upper picture: immunofluorescence detection of Cyp26a1 expression in CD45 + CD3 - CD122 + NK cells isolated from the uterus on gd5 (63 x objective); lower picture: confocal photography of Cyp26a1 + NK cells and Cyp26a1 - NK cells isolated from the uterus on gd5 (20 x and 63 x objective). Data were means +- SEM from three biological replicates, *represents P < .05, **represents P < .01 (two-tailed unpaired Student's t test) (A and C). Data were representative of two (A, B and C) or three (E, F and G) independent experiments

CYP26A1 Antibody in Flow Cytometry (Flow) — Figure 3 Knockdown of CYP26A1 in uterus affects embryo implantation and the balance of uterine M1-like and M2-like macrophages. (A) Representative macroscopic views of the uterus injected with Control-MO or Cyp26a1-MO. Arrows indicates the injection site. (B) Statistics of embryo implantation sites on the MO-injection side (Control-MO, n=10; Cyp26a1-MO, n=17). (C) H&E staining of tissue sections at the implantation sites. Scale bar, 100 um. Arrows indicates the implantation sites. (D, E) FCM analysis of the ratio of uterine M1-like and M2-like macrophages on the MO-injection side (n=7). Error bars represent means +- SEM; two-tailed unpaired t-test, ns, not significant, **P < 0.01 or ***P < 0.001.

CYP26A1 Antibody in Flow Cytometry (Flow) — 3 FIGURE Transcriptome sequencing and differentially expressed gene analysis (A) Flow chart of transcriptome sequencing. (B). Flow cytometric sorting diagram of transcriptome sequencing samples, Cyp26a1 + NK cells (CD45 + CD3 - CD122 + Cyp26a1 + ) and Cyp26a1 - NK cells (CD45 + CD3 - CD122 + Cyp26a1 - ) were isolated from the mouse uterus on gd5. (C) Sample consistency analysis graph, 'P2 and P3' represented Cyp26a1 + NK cells and 'N2 and N3' represented Cyp26a1 - NK cells in the C, D and E. (D) Volcano plot map. (E) Hierarchical clustering demonstrating a distinguishable gene expression pattern between Cyp26a1 + NK cells and Cyp26a1 - NK cells

Product Details

PA5-24602

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

-

Immunocytochemistry (ICC/IF)

-

View 2 publications 2 publications

Flow Cytometry (Flow)

1:10-1:50

View 3 publications 3 publications

Miscellaneous PubMed (MISC)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide between 467-497 amino acids from the C-terminal region of human CYP26A1

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2 mg/mL

Purification

Size-exclusion - Dialysis, Ammonium sulfate precipitation

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2542102

Target Information

CYP26A1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein acts on retinoids, including all-trans-retinoic acid (RA), with both 4-hydroxylation and 18-hydroxylation activities. This enzyme regulates the cellular level of retinoic acid which is involved in regulation of gene expression in both embryonic and adult tissues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Retinol Binding Protein 7 Promotes Adipogenesis in vitro and Regulates Expression of Genes Involved in Retinol Metabolism.

Frontiers in cell and developmental biology

Kim DH,Ahn J,Suh Y,Ziouzenkova O,Lee JW,Lee K

PA5-24602 was used in Western Blotting to indicate critical RBP7 functions in adipocytes: regulation of transcriptional activity of RARE and adipocytes differentiation, potentially providing a new target for obesity therapy.

Sat Jul 16 00:00:00 UTC 2022

Cytochrome P450 26A1 regulates the clusters and killing activity of NK cells during the peri-implantation period.

Journal of cellular and molecular medicine

Li DD,Ji WH,Wei DP,Gu AQ,Song ZH,Fang WN,Meng CY,Yang Y,Peng JP

PA5-24602 was used in Flow cytometry/Cell sorting to investigate the effects of cytochrome P450 26A1 (CYP26A1) on the subsets and killing activity of natural killer (NK) cells.

Fri Apr 01 00:00:00 UTC 2022

A novel Cytochrome P450 26A1 expressing NK cell subset at the mouse maternal-foetal interface.

Journal of cellular and molecular medicine

Wei DP,Li DD,Gu AQ,Ji WH,Yang Y,Peng JP

PA5-24602 was used in Western Blotting, Immunocytochemistry, and Flow Cytometry to show that there is a novel NK cell subset of Cyp26a1+ NK cells in mouse uterus and Cyp26a1 can regulate the gene expression of Gzma, Klrg1 and Fcgr4 in the Cyp26a1+ NK cells.

Mon Feb 01 00:00:00 UTC 2021

A novel Cytochrome P450 26A1 expressing NK cell subset at the mouse maternal-foetal interface.

Journal of cellular and molecular medicine

Wei DP,Li DD,Gu AQ,Ji WH,Yang Y,Peng JP

PA5-24602 was used in Western Blotting, Immunocytochemistry, and Flow Cytometry to show that there is a novel NK cell subset of Cyp26a1+ NK cells in mouse uterus and Cyp26a1 can regulate the gene expression of Gzma, Klrg1 and Fcgr4 in the Cyp26a1+ NK cells.

Mon Feb 01 00:00:00 UTC 2021

A novel Cytochrome P450 26A1 expressing NK cell subset at the mouse maternal-foetal interface.

Journal of cellular and molecular medicine

Wei DP,Li DD,Gu AQ,Ji WH,Yang Y,Peng JP

PA5-24602 was used in Western Blotting, Immunocytochemistry, and Flow Cytometry to show that there is a novel NK cell subset of Cyp26a1+ NK cells in mouse uterus and Cyp26a1 can regulate the gene expression of Gzma, Klrg1 and Fcgr4 in the Cyp26a1+ NK cells.

Mon Feb 01 00:00:00 UTC 2021

A novel Cytochrome P450 26A1 expressing NK cell subset at the mouse maternal-foetal interface.

Journal of cellular and molecular medicine

Wei DP,Li DD,Gu AQ,Ji WH,Yang Y,Peng JP

PA5-24602 was used in Western Blotting, Immunocytochemistry, and Flow Cytometry to show that there is a novel NK cell subset of Cyp26a1+ NK cells in mouse uterus and Cyp26a1 can regulate the gene expression of Gzma, Klrg1 and Fcgr4 in the Cyp26a1+ NK cells.

Mon Feb 01 00:00:00 UTC 2021

A novel Cytochrome P450 26A1 expressing NK cell subset at the mouse maternal-foetal interface.

Journal of cellular and molecular medicine

Wei DP,Li DD,Gu AQ,Ji WH,Yang Y,Peng JP

PA5-24602 was used in Western Blotting, Immunocytochemistry, and Flow Cytometry to show that there is a novel NK cell subset of Cyp26a1+ NK cells in mouse uterus and Cyp26a1 can regulate the gene expression of Gzma, Klrg1 and Fcgr4 in the Cyp26a1+ NK cells.

Mon Feb 01 00:00:00 UTC 2021

A novel Cytochrome P450 26A1 expressing NK cell subset at the mouse maternal-foetal interface.

Journal of cellular and molecular medicine

Wei DP,Li DD,Gu AQ,Ji WH,Yang Y,Peng JP

PA5-24602 was used in Western Blotting, Immunocytochemistry, and Flow Cytometry to show that there is a novel NK cell subset of Cyp26a1+ NK cells in mouse uterus and Cyp26a1 can regulate the gene expression of Gzma, Klrg1 and Fcgr4 in the Cyp26a1+ NK cells.

Mon Feb 01 00:00:00 UTC 2021

Cytochrome P450 26A1 Modulates the Polarization of Uterine Macrophages During the Peri-Implantation Period.

Frontiers in immunology

Ji WH,Li DD,Wei DP,Gu AQ,Yang Y,Peng JP

Published figure using CYP26A1 polyclonal antibody (Product # PA5-24602) in Flow Cytometry

Thu Feb 10 00:00:00 UTC 2022

Cytochrome P450 26A1 Modulates the Polarization of Uterine Macrophages During the Peri-Implantation Period.

Frontiers in immunology

Ji WH,Li DD,Wei DP,Gu AQ,Yang Y,Peng JP

Published figure using CYP26A1 polyclonal antibody (Product # PA5-24602) in Flow Cytometry

Thu Feb 10 00:00:00 UTC 2022

Cytochrome P450 26A1 Modulates the Polarization of Uterine Macrophages During the Peri-Implantation Period.

Frontiers in immunology

Ji WH,Li DD,Wei DP,Gu AQ,Yang Y,Peng JP

Published figure using CYP26A1 polyclonal antibody (Product # PA5-24602) in Flow Cytometry

Thu Feb 10 00:00:00 UTC 2022

Cytochrome P450 26A1 Modulates the Polarization of Uterine Macrophages During the Peri-Implantation Period.

Frontiers in immunology

Ji WH,Li DD,Wei DP,Gu AQ,Yang Y,Peng JP

Published figure using CYP26A1 polyclonal antibody (Product # PA5-24602) in Flow Cytometry

Thu Feb 10 00:00:00 UTC 2022

Bioinformatics

Protein Aliases: CYP26A1; Cytochrome P450 26A1; Cytochrome P450 retinoic acid-inactivating 1; cytochrome P450, 26, retinoic acid A1; cytochrome P450, family 26, subfamily A, polypeptide 1; cytochrome P450, subfamily XXVIA, polypeptide 1; Cytochrome P450RAI; hP450RAI; P450, retinoic acid-inactivating, 1; Retinoic acid 4-hydroxylase; retinoic acid hydrolase; Retinoic acid-metabolizing cytochrome

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Gene Aliases: CP26; CYP26; CYP26A1; P450RA; P450RAI; P450RAI1; RAH

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UniProt ID: (Human) O43174, (Mouse) O55127

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Entrez Gene ID: (Human) 1592, (Mouse) 13082

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retinoic acid binding iron ion binding retinoic acid 4-hydroxylase activity oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen oxygen binding heme binding monooxygenase activity oxidoreductase activity oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen fluorene oxygenase activity mono-butyltin dioxygenase activity tri-n-butyltin dioxygenase activity di-n-butyltin dioxygenase activity methylsilanetriol hydroxylase activity methyl tertiary butyl ether 3-monooxygenase activity 4-nitrocatechol 4-monooxygenase activity 4-chlorophenoxyacetate monooxygenase activity tert-butanol 2-monooxygenase activity alpha-pinene monooxygenase activity dimethylsilanediol hydroxylase activity ammonia monooxygenase activity hydroxymethylsilanetriol oxidase activity 2-hydroxyisobutyrate 3-monooxygenase activity alpha-pinene dehydrogenase activity bisphenol A hydroxylase B activity 2,2-bis(4-hydroxyphenyl)-1-propanol hydroxylase activity 9-fluorenone-3,4-dioxygenase activity anthracene 9,10-dioxygenase activity 2-(methylthio)benzothiazole monooxygenase activity 2-hydroxybenzothiazole monooxygenase activity benzothiazole monooxygenase activity 2,6-dihydroxybenzothiazole monooxygenase activity pinacolone 5-monooxygenase activity thioacetamide S-oxygenase activity thioacetamide S-oxide S-oxygenase activity endosulfan monooxygenase I activity N-nitrodimethylamine hydroxylase activity 4-(1-ethyl-1,4-dimethyl-pentyl)phenol monoxygenase activity endosulfan ether monooxygenase activity pyrene 4,5-monooxygenase activity pyrene 1,2-monooxygenase activity 1-hydroxypyrene 6,7-monooxygenase activity 1-hydroxypyrene 7,8-monooxygenase activity phenylboronic acid monooxygenase activity spheroidene monooxygenase activity metal ion binding

vitamin metabolic process xenobiotic metabolic process retinoic acid catabolic process retinoic acid metabolic process negative regulation of retinoic acid receptor signaling pathway oxidation-reduction process central nervous system development anterior/posterior pattern specification neural crest cell development retinoic acid receptor signaling pathway

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