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CYP2B6 Antibody (PA5-89950) in IHC (P)
Immunohistochemistry analysis of CYP2B6 in paraffin-embedded human breast cancer using. Samples were incubated with CYP2B6 Polyclonal antibody (Product # PA5-89950) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Product Details
PA5-89950
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 160-340 of human CYP2B6 (NP_0007581)
Conjugate
Form
Liquid
Concentration
0.27 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2805861
Product Specific Information
Immunogen sequence: SKGALMDPTF LFQSITANII CSIVFGKRFH YQDQEFLKML NLFYQTFSLI SSVFGQLFEL FSGFLKYFPG AHRQVYKNLQ EINAYIGHSV EKHRETLDPS APKDLIDTYL LHMEKEKSNA HSEFSHQNLN LNTLSLFFAG TETTSTTLRY GFLLMLKYPH VAERVYREIE QVIGPHRPPE L; Positive Samples: NCI-H460, HT-29, Mouse liver; Cellular Location: Endoplasmic reticulum membrane, Microsome membrane, Peripheral membrane protein
Target Information
This gene, CYP2B6, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. The enzyme is known to metabolize some xenobiotics, such as the anti-cancer drugs cyclophosphamide and ifosphamide. Transcript variants for this gene have been described; however, it has not been resolved whether these transcripts are in fact produced by this gene or by a closely related pseudogene, CYP2B7. Both the gene and the pseudogene are located in the middle of a CYP2A pseudogene found in a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 1,4-cineole 2-exo-monooxygenase; CYPIIB3; CYPIIB6; Cytochrome P450 2B3; Cytochrome P450 2B6; Cytochrome P450 IIB1; cytochrome P450, family 2, subfamily B, polypeptide 6; cytochrome P450, subfamily IIB (phenobarbital-inducible), polypeptide 6; cytochrome P450IIB3
Gene Aliases: CPB6; CYP2B; Cyp2b-3; Cyp2b3; CYP2B6; CYP2B7; CYP2B7P; CYPIIB6; EFVM; IIB1; P450
UniProt ID: (Human) P20813, (Rat) P13107
Entrez Gene ID: (Human) 1555, (Rat) 286953
monooxygenase activity
iron ion binding
arachidonic acid epoxygenase activity
steroid hydroxylase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen
oxygen binding
heme binding
aromatase activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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