Proteintech

CYP3A4 Polyclonal Antibody

CYP3A4 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 8

CYP3A4 Antibody (18227-1-AP) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HepG2 cells using 18227-1-AP (CYP3A4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

CYP3A4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

Product Details

18227-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 34 publications 34 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 8 publications 8 publications

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Not applicable (N/A)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

CYP3A4 Fusion Protein Ag13074 (154-503 aa encoded by BC101631)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.47 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: DVLVRNLRR EAETGKPVTL KDVFGAYSMD VITSTSFGVN IDSLNNPQDP FVENTKKLLR FDFLDPFFLS ITVFPFLIPI LEVLNICVFP REVTNFLRKS VKRMKESRLE DTQKHRVDFL QLMIDSQNSK ETESHKALSD LELVAQSIIF IFAGYETTSS VLSFIMYELA THPDVQQKLQ EEIDAVLPNK APPTYDTVLQ MEYLDMVVNE TLRLFPIAMR LERVCKKDVE INGMFIPKGV VVMIPSYALH RDPKYWTEPE KFLPERFSKK NKDNIDPYIY TPFGSGPRNC IGMRFALMNM KLALIRVLQN FSFKPCKETQ IPLKLSLGGL LQPEKPVVLK VESRDGTVSG A (154-503 aa encoded by BC101631)

Target Information

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. This enzyme is involved in the metabolism of approximately half the drugs in use today, including acetaminophen, codeine, cyclosporin A, diazepam and erythromycin. The enzyme also metabolizes some steroids and carcinogens. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Previously another CYP3A gene, CYP3A3, was thought to exist; however, it is now thought that this sequence represents a transcript variant of CYP3A4. Alternatively spliced transcript variants encoding different isoforms have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Effects of tetrahydroxystilbene glucoside on mouse liver cytochrome P450 enzyme expressions.

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Wang YY,Yang J,Liu H,Lin FQ,Shi JS,Zhang F

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Alteration in the expression of cytochrome P450s (CYP1A1, CYP2E1, and CYP3A11) in the liver of mouse induced by microcystin-LR.

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Revival, characterization, and hepatitis B virus infection of cryopreserved human fetal hepatocytes.

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Long-term maintenance of human fetal hepatocytes and prolonged susceptibility to HBV infection by co-culture with non-parenchymal cells.

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Mushroom Ganoderma lucidum prevents colitis-associated carcinogenesis in mice.

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Bioinformatics

Protein Aliases: 1,4-cineole 2-exo-monooxygenase; 1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; Albendazole monooxygenase (sulfoxide-forming); Albendazole sulfoxidase; Cholesterol 25-hydroxylase; CYP; CYPIIIA3; CYPIIIA4; Cytochrome P450 3A3; Cytochrome P450 3A4; Cytochrome P450 HLp; Cytochrome P450 NF-25; cytochrome P450, family 3, subfamily A, polypeptide 4; cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 3; cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 4; Cytochrome P450-PCN1; glucocorticoid-inducible P450; MGC126680; Nifedipine oxidase; P450; P450-III, steroid inducible; P450-PCN1; Quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase

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Gene Aliases: CP33; CP34; CYP3A; CYP3A3; CYP3A4; CYPIIIA3; CYPIIIA4; HLP; NF-25; P450C3; P450PCN1

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UniProt ID: (Human) P08684

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Entrez Gene ID: (Human) 1576

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Function(s)

monooxygenase activity steroid binding iron ion binding steroid hydroxylase activity oxidoreductase activity oxygen binding enzyme binding heme binding vitamin D3 25-hydroxylase activity taurochenodeoxycholate 6alpha-hydroxylase activity caffeine oxidase activity albendazole monooxygenase activity quinine 3-monooxygenase activity testosterone 6-beta-hydroxylase activity aromatase activity vitamin D 24-hydroxylase activity

Process(es)

lipid metabolic process steroid catabolic process xenobiotic metabolic process steroid metabolic process androgen metabolic process alkaloid catabolic process monoterpenoid metabolic process drug metabolic process calcitriol biosynthetic process from calciol vitamin D metabolic process drug catabolic process exogenous drug catabolic process heterocycle metabolic process oxidation-reduction process oxidative demethylation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CYP3A4 Polyclonal Antibody

CYP3A4 Antibody (18227-1-AP) in ICC/IF

Product Details

18227-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Miscellaneous PubMed (MISC)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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