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Invitrogen
CYP3A5 Monoclonal Antibody (F18 P3 B6)
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FIGURE: 1 / 4
CYP3A5 Antibody (MA3-033) in ICC/IF
Immunofluorescence analysis of Cytochrome P450-3A5 was done on 70% confluent log phase HepG2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Cytochrome P450 3A5 (F18 P3 B6) Mouse Monoclonal Antibody (Product # MA3-033) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluo... View More
Product Details
MA3-033
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human
Published species
Human,
Non-human primate
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
F18 P3 B6
Immunogen
Synthetic peptide corresponding to residues E S R D G T L S G E, conjugated to ovalbumin.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2090513
Product Specific Information
MA3-033 detects Cytochrome P450 3A5 from HEK293 and HepG2 human cell lines, and from bovine liver and pancreas samples.
MA3-033 has been successfully used in Western blot applications. By Western blot, this antibody detects a ~54 kDa band representing Cytochrome P450 3A5.
The MA3-033 immunogen is a synthetic peptide corresponding to residues E S R D G T L S G E, conjugated to ovalbumin.
Target Information
CYP3A5 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. The enzyme metabolizes drugs such as nifedipine and cyclosporine as well as the steroid hormones testosterone, progesterone and androstenedione.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: aryl hydrocarbon hydroxylase; CYPIIIA28; CYPIIIA5; cytochrome P450 3A28; Cytochrome P450 3A5; cytochrome P450 HLp2; cytochrome P450, family 3, subfamily A, polypeptide 5; cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 5; Cytochrome P450-PCN3; flavoprotein-linked monooxygenase; HLp2; microsomal monooxygenase; P450-PCN3; xenobiotic monooxygenase
Gene Aliases: BOS_22940; BOS_22999; CP35; CYP3A28; CYP3A4; CYP3A5; CYPIIIA5; P450PCN3; PCN3
UniProt ID: (Human) P20815
Entrez Gene ID: (Bovine) 282214, (Bovine) 526682, (Human) 1577
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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