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CYP51A1 Polyclonal Antibody

CYP51A1 Antibody in Immunocytochemistry (ICC/IF)

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CYP51A1 Antibody (13431-1-AP) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol ) fixed PC-3 cells using 13431-1-AP (CYP51A1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

CYP51A1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

13431-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 20 publications 20 publications

Immunocytochemistry (ICC/IF)

1:50

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

CYP51A1 Fusion Protein Ag4235 (146-509 aa encoded by BC032322)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.13 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: GKGVAYDVP NPVFLEQKKM LKSGLNIAHF KQHVSIIEKE TKEYFESWGE SGEKNVFEAL SELIILTASH CLHGKEIRSQ LNEKVAQLYA DLDGGFSHAA WLLPGWLPLP SFRRRDRAHR EIKDIFYKAI QKRRQSQEKI DDILQTLLDA TYKDGRPLTD DEVAGMLIGL LLAGQHTSST TSAWMGFFLA RDKTLQKKCY LEQKTVCGEN LPPLTYDQLK DLNLLDRCIK ETLRLRPPVM IMMRMARTPQ TVAGYTIPPG HQVCVSPTVN QRLKDSWVER LDFNPDRYLQ DNPASGEKFA YVPFGAGRHR CIGENFAYVQ IKTIWSTMLR LYEFDLIDGY FPTVNYTTMI HTPENPVIRY KRRSK (146-509 aa encoded by BC032322)

Target Information

P450 enzymes constitute a family of monooxygenase enzymes that are involved in the metabolism of a wide array of endogenous and xenobiotic compounds. Several P450 enzymes have been classified by sequence similarities as members of the CYP1A and CYP2A subfamilies. CYP51A1 (cytochrome P450, family 51, subfamily A, polypeptide 1), also known as LDM (lanosterol 14-alpha demethylase) or cytochrome P450-14DM, is a 503 amino acid protein localized to endoplasmic reticulum membrane. CYP51A1 is an important enzyme for zymosterol and steroid biosynthesis. CYP51A1 catalyzes C14-demethylation of lanosterol, transforming lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol. CYP51A1 is ubiquitously expressed, with highest levels found in liver, ovary, testis, lung, kidney and prostate.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Bicyclol attenuates high fat diet-induced non-alcoholic fatty liver disease/non-alcoholic steatohepatitis through modulating multiple pathways in mice.

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Wu J,Jia S,Xu B,Yao X,Shao J,Yao J,Cen D,Yao X

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Schwann Cells Accelerate Osteogenesis via the Mif/CD74/FOXO1 Signaling Pathway In Vitro.

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POST1/C12ORF49 regulates the SREBP pathway by promoting site-1 protease maturation.

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Vascular biotransformation of organic nitrates is independent of cytochrome P450 monooxygenases.

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Lopez M,Malacarne PF,Gajos-Draus A,Ding X,Daiber A,Lundberg JO,Offermanns S,Brandes RP,Rezende F

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MMAB promotes negative feedback control of cholesterol homeostasis.

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Goedeke L,Canfrán-Duque A,Rotllan N,Chaube B,Thompson BM,Lee RG,Cline GW,McDonald JG,Shulman GI,Lasunción MA,Suárez Y,Fernández-Hernando C

Mon Nov 08 00:00:00 UTC 2021

Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.

Neuron

Li X,Zhang J,Li D,He C,He K,Xue T,Wan L,Zhang C,Liu Q

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Endogenous sterol intermediates of the mevalonate pathway regulate HMGCR degradation and SREBP-2 processing.

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Bioinformatics

Protein Aliases: CYP51; CYP51A1; CYPLI; Cytochrom P450 Lanosterol 14 alpha-demethylase; Cytochrome P450 51; Cytochrome P450 51A1; cytochrome P450 Lanosterol 14 alpha-demethylase; cytochrome P450, 51; cytochrome P450, 51 (lanosterol 14-alpha-demethylase); cytochrome P450, family 51, subfamily A, polypeptide 1; cytochrome P450, subfamily 51; Cytochrome P450-14DM; Cytochrome P45014DM; Cytochrome P450LI; Lanosterol 14-alpha demethylase; lanosterol 14-alpha-demethylase; LDM; P450 14DM; P450-14DM; P45014DM; P450LI; Sterol 14-alpha demethylase; Sterol 14-alpha demethylase P450

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Gene Aliases: AI426508; CP51; CYP51; CYP51A1; CYPL1; CYPLI; LDM; P450-14DM; P450L1; P450LI; RATCP14DM

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UniProt ID: (Human) Q16850, (Mouse) Q8K0C4, (Rat) Q64654

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Entrez Gene ID: (Human) 1595, (Mouse) 13121, (Rat) 25427

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Function(s)

iron ion binding sterol 14-demethylase activity heme binding monooxygenase activity oxidoreductase activity oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen metal ion binding

Process(es)

lipid metabolic process steroid biosynthetic process cholesterol biosynthetic process steroid metabolic process cholesterol metabolic process sterol biosynthetic process cholesterol biosynthetic process via 24,25-dihydrolanosterol oxidation-reduction process demethylation regulation of cholesterol biosynthetic process sterol metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CYP51A1 Polyclonal Antibody

CYP51A1 Antibody (13431-1-AP) in ICC/IF

Product Details

13431-1-AP

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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