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FIGURE: 1 / 1
CaMKI Antibody (MA5-38075) in WB
Western blot analysis of CaMKI in extracts of various cell lines. Samples were incubated with CaMKI Monoclonal antibody (Product # MA5-38075) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1 min.
Product Details
MA5-38075
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
4P4K9
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CaMKI (NP_0036471)
Conjugate
Form
Liquid
Concentration
0.89 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2897993
Product Specific Information
Positive Samples: HepG2, THP-1, Mouse lung, Mouse liver, Mouse brain, Rat lung, Rat liver, Rat brain
Immunogen sequence: MLGAVEGPRW KQAEDIRDIY DFRDVLGTGA FSEVILAEDK RTQKLVAIKC IAKEALEGKE GSMENEIAVL HKIKHPNIVA LDDIYESGGH LYLIMQLVSG
Target Information
CAMK1 (CaMK1) is a serine/threonine kinase that is a member of the calcium/calmodulin-dependent protein kinase family. It is involved in cell differentiation, nervous system development, and signal transduction. CAMK1 is expressed in many tissues and is a component of a calmodulin-dependent protein kinase cascade. Calcium/calmodulin directly activates calcium/calmodulin-dependent protein kinase I by binding to the enzyme and indirectly promotes the phosphorylation and synergistic activation of the enzyme by calcium/calmodulin-dependent protein kinase I kinase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: calcium/calmodulin-dependent protein kinase I; Calcium/calmodulin-dependent protein kinase type 1; CaM kinase I; CaM kinase I alpha; CaM-KI; CAMK1-PEN; CaMKI-alpha; G alpha interacting protein; MGC120317; MGC120318; regulator of G-protein signalling 19
Gene Aliases: AI505105; CAMK1; CAMKI; CaMKIalpha; D6Ertd263e; Gaip
UniProt ID: (Human) Q14012, (Rat) Q63450, (Mouse) Q91YS8
Entrez Gene ID: (Human) 8536, (Rat) 171503, (Mouse) 52163
protein phosphorylation
nucleocytoplasmic transport
signal transduction
positive regulation of neuron projection development
negative regulation of protein binding
regulation of protein localization
positive regulation of peptidyl-serine phosphorylation
regulation of protein binding
positive regulation of transcription from RNA polymerase II promoter
positive regulation of protein export from nucleus
regulation of muscle cell differentiation
positive regulation of muscle cell differentiation
positive regulation of synapse structural plasticity
positive regulation of syncytium formation by plasma membrane fusion
positive regulation of dendritic spine development
positive regulation of protein serine/threonine kinase activity
positive regulation of protein acetylation
regulation of histone H3-K9 acetylation
cell cycle
multicellular organism development
nervous system development
metabolic process
phosphorylation
cell differentiation
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