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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • CaMKII alpha Antibodies

          Invitrogen

          CaMKII alpha Monoclonal Antibody (6G9)

          Advanced Verification
          38 Published Figures
          116 References
          View all (33) CaMKII alpha antibodies
          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite CaMKII alpha Monoclonal Antibody (6G9)

          • Antibody Testing Data (3)
          • Published Figures (38)
          • Advanced Verification (2)
          CaMKII alpha Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          CaMKII alpha Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 43

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          CaMKII alpha Antibody (MA1-048) in WB

          Western blot was performed using Anti-CaMKII alpha Monoclonal Antibody (6G9) (Product # MA1-048) and a band at ~50 kDa corresponding to CaMKII alpha was observed in Mouse and Rat Brain, Mouse and Rat Cerebellum in comparison to other tissues which are reported to be negative. Tissue extracts (30 µg lysate) of Mouse Brain (Lane 1), Rat Brain (Lane 2), Mouse Cerebellum (Lane 3), Rat Cerebellum (Lane 4), Mouse Skeletal Muscle (Lane 5), Rat Skeletal Muscle (Lane 6), Mouse Heart (Lane 7) and Rat Heart (Lane 8) were electrophoresed using Novex® ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          View Product

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          CaMKII alpha Antibody in Western Blot (WB)
          CaMKII alpha Antibody in Western Blot (WB)
          CaMKII alpha Antibody in Western Blot (WB)
          CaMKII alpha Antibody in Immunocytochemistry (ICC/IF)
          CaMKII alpha Antibody in Immunocytochemistry (ICC/IF)
          CaMKII alpha Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
          CaMKII alpha Antibody in Immunocytochemistry (ICC/IF)
          CaMKII alpha Antibody in Immunocytochemistry (ICC/IF)
          CaMKII alpha Antibody in Immunocytochemistry (ICC/IF)
          CaMKII alpha Antibody in Immunohistochemistry (IHC)
          CaMKII alpha Antibody in Immunohistochemistry (IHC)
          CaMKII alpha Antibody in Immunohistochemistry (IHC)
          CaMKII alpha Antibody in Immunohistochemistry (IHC)
          CaMKII alpha Antibody in Immunohistochemistry (IHC)
          CaMKII alpha Antibody in Immunohistochemistry (IHC)
          CaMKII alpha Antibody in Immunohistochemistry (IHC)
          CaMKII alpha Antibody in Immunohistochemistry (IHC)
          CaMKII alpha Antibody in Immunohistochemistry (IHC)
          CaMKII alpha Antibody in Immunohistochemistry (IHC)
          CaMKII alpha Antibody in Immunohistochemistry (IHC)
          CaMKII alpha Antibody in Immunohistochemistry (IHC)
          CaMKII alpha Antibody in Immunohistochemistry (IHC)
          CaMKII alpha Antibody in Western Blot (WB)
          CaMKII alpha Antibody in Western Blot (WB)
          CaMKII alpha Antibody in Western Blot, Immunoprecipitation (WB, IP)
          CaMKII alpha Antibody in Western Blot (WB)
          CaMKII alpha Antibody in Western Blot (WB)
          CaMKII alpha Antibody in Western Blot (WB)
          CaMKII alpha Antibody in Western Blot (WB)
          CaMKII alpha Antibody in Western Blot (WB)
          CaMKII alpha Antibody in Western Blot (WB)
          CaMKII alpha Antibody in Western Blot (WB)
          CaMKII alpha Antibody in Western Blot (WB)
          CaMKII alpha Antibody in Western Blot (WB)
          CaMKII alpha Antibody in Western Blot (WB)
          CaMKII alpha Antibody in Western Blot, Immunoprecipitation (WB, IP)
          CaMKII alpha Antibody in Western Blot (WB)
          CaMKII alpha Antibody in Western Blot (WB)
          CaMKII alpha Antibody in Western Blot (WB)
          CaMKII alpha Antibody in Western Blot (WB)
          CaMKII alpha Antibody
          CaMKII alpha Antibody

          Product Details

          MA1-048

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.5-1 µg/mL
          View 50 publications 50 publications

          Immunohistochemistry (IHC)

          Assay-dependent
          View 42 publications 42 publications

          Immunohistochemistry (Frozen) (IHC (F))

          -
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          -
          View 15 publications 15 publications

          Immunoprecipitation (IP)

          Assay-dependent
          View 5 publications 5 publications

          Miscellaneous PubMed (MISC)

          -
          View 3 publications 3 publications
          Product Specifications

          Species Reactivity

          Mouse, Rat

          Published species

          Human, Mouse, Rat

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          6G9

          Immunogen

          Partially purified, rat CaM kinase II.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1.17 mg/mL

          Purification

          Protein A

          Storage buffer

          PBS

          Contains

          0.05% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_325403

          Product Specific Information

          MA1-048 detects CaM kinase II from rat and mouse tissues. This antibody has been shown to detect both the phosphorylated and non-phosphorylated alpha subunit of CaM kinase II only. MA1-048 does not detect the ~60 kDa beta subunit in either phosphorylation state.

          MA1-048 has been successfully used in Western blot, immunohistochemistry, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects an ~50 kDa protein representing the alpha subunit of CaM kinase II. Immunofluorescence staining of CaM kinase II in rat hippocampal cells with MA1-048 results in intense staining of cell somas and neuropil areas, while nuclei are only lightly stained.

          The MA1-048 immunogen is partially purified, rat CaM kinase II.

          Target Information

          CAMK2A (CaMKII alpha) is a serine/threonine kinase that regulates a number of cellular functions including neuronal processes, gene expression, carbohydrate metabolism, cytoskeletal function, and calcium homeostatsis. Autophosphorylation of threonine 286 in the presence of Ca2+ and calmodulin activates CAMK2A with the ability to switch from calmodulin-dependent to a calmodulin independent activity. CaMKII is an ubiquitously expressed serine/threonine protein kinase that is activated by Ca2+/calmodulin (CaM) binding and autophosphorylation and has been implicated in regulation of the cell cycle and transcription. It is involved in dendritic spine and synapse formation, neuronal plasticity and regulation of sarcoplasmic reticulum Ca2+ transport in skeletal muscle. In neurons, plays an essential structural role in the reorganization of the actin cytoskeleton during plasticity by binding and bundling actin filaments in a kinase-independent manner.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: alpha CaM kinase II; alpha-CaMKII; Ca2+/calmodulin-dependent protein kinase II alpha; Calci; calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha; calcium/calmodulin-dependent protein kinase II alpha subunit; calcium/calmodulin-dependent protein kinase type II alpha chain; Calcium/calmodulin-dependent protein kinase type II subunit alpha; CaM kinase II subunit alpha; caM-kinase II alpha chain; CaMK II; caMK-II subunit alpha; EC 2.7.11.17; OTTHUMP00000165787; OTTHUMP00000165788; Phospho-CaMKI

          View more View less

          Gene Aliases: Camk2a; CaMKII; mKIAA0968; PK2CDD; PKCCD; R74975

          View more View less

          UniProt ID: (Mouse) P11798, (Rat) P11275

          View more View less

          Entrez Gene ID: (Mouse) 12322, (Rat) 25400

          View more View less

          Function(s)
          nucleotide binding protein kinase activity protein serine/threonine kinase activity calmodulin-dependent protein kinase activity protein binding calmodulin binding ATP binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups GTPase activating protein binding glutamate receptor binding protein homodimerization activity
          Process(es)
          G1/S transition of mitotic cell cycle response to ischemia protein phosphorylation calcium ion transport positive regulation of cardiac muscle cell apoptotic process phosphorylation peptidyl-serine phosphorylation ionotropic glutamate receptor signaling pathway angiotensin-activated signaling pathway protein autophosphorylation regulation of neurotransmitter secretion regulation of neuronal synaptic plasticity positive regulation of NF-kappaB transcription factor activity positive regulation of calcium ion transport regulation of mitochondrial membrane permeability involved in apoptotic process
          It has to be done as per old AB suggested Products section.
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