Invitrogen

CaMKII alpha Monoclonal Antibody (Cba-2)

CaMKII alpha Antibody in Western Blot (WB)

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CaMKII alpha Antibody (13-7300) in WB

Western blot was performed using Anti-CaMKII alpha Monoclonal Antibody (Cba-2) (Product # 13-7300) and a band at ~50 kDa corresponding to CaMKII alpha was observed in Mouse Brain in comparison to other tissues which are reported to be negative. Tissue extracts (30 µg lysate) of Mouse Brain (Lane 1), Mouse Skeletal Muscle (Lane 2) and Mouse Heart (Lane 3) were electrophoresed using Novex® NuPAGE® 4-12% % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2... View More

CaMKII alpha Antibody in Immunohistochemistry (IHC)

CaMKII alpha Antibody in Western Blot, Immunoprecipitation (WB, IP)

CaMKII alpha Antibody in Flow Cytometry (Flow)

Product Image Gallery

Product Details

13-7300

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-1 µg/mL

View 16 publications 16 publications

Immunohistochemistry (IHC)

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

1-5 µg/mL

Immunocytochemistry (ICC/IF)

View 5 publications 5 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

ELISA (ELISA)

0.1-1 µg/mL

Immunoprecipitation (IP)

2 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Fish, Human, Mouse, Sheep, Rat

Published species

Hamster, Human, Mouse, Rat

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

Cba-2

Immunogen

Purified rat brain CaM Kinase.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533032

Product Specific Information

This antibody is specific for alpha subunit of CaM Kinase II. No significant reactivity was seen with the B, B1, yB, dA, dB, and dC isoforms. The epitope is located in the regulatory domain.

Target Information

CAMK2A (CaMKII alpha) is a serine/threonine kinase that regulates a number of cellular functions including neuronal processes, gene expression, carbohydrate metabolism, cytoskeletal function, and calcium homeostatsis. Autophosphorylation of threonine 286 in the presence of Ca2+ and calmodulin activates CAMK2A with the ability to switch from calmodulin-dependent to a calmodulin independent activity. CaMKII is an ubiquitously expressed serine/threonine protein kinase that is activated by Ca2+/calmodulin (CaM) binding and autophosphorylation and has been implicated in regulation of the cell cycle and transcription. It is involved in dendritic spine and synapse formation, neuronal plasticity and regulation of sarcoplasmic reticulum Ca2+ transport in skeletal muscle. In neurons, plays an essential structural role in the reorganization of the actin cytoskeleton during plasticity by binding and bundling actin filaments in a kinase-independent manner.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Excitatory somatostatin interneurons in the dentate gyrus drive a widespread seizure network in cortical dysplasia.

Signal transduction and targeted therapy

Zheng Y,Xu C,Sun J,Ming W,Dai S,Shao Y,Qiu X,Li M,Shen C,Xu J,Fei F,Fang J,Jiang X,Zheng G,Hu W,Wang Y,Wang S,Ding M,Chen Z

13-7300 was used in Immunohistochemistry to quantify the epileptogenicity of the hippocampus in patients with late-stage cortical dysplasia, and investigated the cellular substrates leading to the epileptic hippocampus.

Wed May 17 00:00:00 UTC 2023

Altered excitatory and decreased inhibitory transmission in the prefrontal cortex of male mice with early developmental disruption to the ventral hippocampus.

Cerebral cortex (New York, N.Y. : 1991)

Nath M,Bhardwaj SK,Srivastava LK,Wong TP

13-7300 was used in Immunohistochemistry-immunofluorescence to highlight mechanisms by which the PFC adapts to early developmental vHPC perturbations, providing insights into schizophrenia circuit pathology.

Thu Jan 05 00:00:00 UTC 2023

Chemogenetic rectification of the inhibitory tone onto hippocampal neurons reverts autistic-like traits and normalizes local expression of estrogen receptors in the Ambra1+/- mouse model of female autism.

Translational psychiatry

Pignataro A,Krashia P,De Introna M,Nobili A,Sabetta A,Stabile F,La Barbera L,D'Addario SL,Ventura R,Cecconi F,D'Amelio M,Ammassari-Teule M

13-7300 was used in ELISA, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to identify an autophagy deficiency-related mechanism of hippocampal neural and hormonal dysregulation which opens novel perspectives for treatments specifically designed for autistic females.

Mon Feb 20 00:00:00 UTC 2023

Repeated Social Defeat Stress Induces HMGB1 Nuclear Export in Prefrontal Neurons, Leading to Social Avoidance in Mice.

Cells

Kitaoka S,Tomohiro A,Ukeshima S,Liu K,Wake H,Kimura SH,Yamamoto Y,Nishibori M,Furuyashiki T

13-7300 was used in Immunohistochemistry to find that the cerebroventricular infusion of HMGB1 proteins blocked stress-induced social avoidance and that HMGB1-neutralizing antibodies augmented repeated social defeat stress-induced social avoidance in mice, suggesting the antidepressive-like effect of HMGB1 in the brain.

Wed Jul 05 00:00:00 UTC 2023

CAMK2/CaMKII activates MLKL in short-term starvation to facilitate autophagic flux.

Autophagy

Zhan Q,Jeon J,Li Y,Huang Y,Xiong J,Wang Q,Xu TL,Li Y,Ji FH,Du G,Zhu MX

13-7300 was used in Western Blot to propose that upon nutrient deprivation, MLKL is activated by CAMK2, which in turn facilitates membrane scission needed for autophagosome maturation, allowing the proper fusion of the autophagosome with lysosome and the subsequent substance degradation.

Fri Apr 01 00:00:00 UTC 2022

CAMK2/CaMKII activates MLKL in short-term starvation to facilitate autophagic flux.

Autophagy

Zhan Q,Jeon J,Li Y,Huang Y,Xiong J,Wang Q,Xu TL,Li Y,Ji FH,Du G,Zhu MX

Fri Apr 01 00:00:00 UTC 2022

CAMK2/CaMKII activates MLKL in short-term starvation to facilitate autophagic flux.

Autophagy

Zhan Q,Jeon J,Li Y,Huang Y,Xiong J,Wang Q,Xu TL,Li Y,Ji FH,Du G,Zhu MX

Fri Apr 01 00:00:00 UTC 2022

Dynamic bi-directional phosphorylation events associated with the reciprocal regulation of synapses during homeostatic up- and down-scaling.

Cell reports

Desch K,Langer JD,Schuman EM

Published figure using CaMKII alpha monoclonal antibody (Product # 13-7300) in Western Blot

Tue Aug 24 00:00:00 UTC 2021

Adult brain neurons require continual expression of the schizophrenia-risk gene Tcf4 for structural and functional integrity.

Translational psychiatry

Sarkar D,Shariq M,Dwivedi D,Krishnan N,Naumann R,Bhalla US,Ghosh HS

13-7300 was used in ELISA, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to identify previously unappreciated gene networks underpinning mature neuronal regulation during the adult lifespan.

Sat Sep 25 00:00:00 UTC 2021

Reciprocal control of excitatory synapse numbers by Wnt and Wnt inhibitor PRR7 secreted on exosomes.

Nature communications

Lee SH,Shin SM,Zhong P,Kim HT,Kim DI,Kim JM,Heo WD,Kim DW,Yeo CY,Kim CH,Liu QS

13-7300 was used in Western Blotting to identify a proximity-dependent, reciprocal mechanism for the regulation of excitatory synapse numbers in local neurons and demonstrate the significance of exosomes in inter-neuronal signalling in the vertebrate brain.

Fri Aug 24 00:00:00 UTC 2018

The Developmental Shift of NMDA Receptor Composition Proceeds Independently of GluN2 Subunit-Specific GluN2 C-Terminal Sequences.

Cell reports

McKay S,Ryan TJ,McQueen J,Indersmitten T,Marwick KFM,Hasel P,Kopanitsa MV,Baxter PS,Martel MA,Kind PC,Wyllie DJA,O'Dell TJ,Grant SGN,Hardingham GE,Komiyama NH

13-7300 was used in Western Blot to study the developmental changes in NMDA receptor composition, and the roles of GluN2A expression levels and GluN2 subtype-specific CTD-driven events in these changes.

Tue Oct 23 00:00:00 UTC 2018

The Developmental Shift of NMDA Receptor Composition Proceeds Independently of GluN2 Subunit-Specific GluN2 C-Terminal Sequences.

Cell reports

McKay S,Ryan TJ,McQueen J,Indersmitten T,Marwick KFM,Hasel P,Kopanitsa MV,Baxter PS,Martel MA,Kind PC,Wyllie DJA,O'Dell TJ,Grant SGN,Hardingham GE,Komiyama NH

Tue Oct 23 00:00:00 UTC 2018

Calmodulin shuttling mediates cytonuclear signaling to trigger experience-dependent transcription and memory.

Nature communications

Cohen SM,Suutari B,He X,Wang Y,Sanchez S,Tirko NN,Mandelberg NJ,Mullins C,Zhou G,Wang S,Kats I,Salah A,Tsien RW,Ma H

13-7300 was used in Immunocytochemistry to show that elimination of γCaMKII prevents activity-dependent expression of key genes (BDNF, c-Fos, Arc), inhibits persistent synaptic strengthening, and impairs spatial memory in vivo.

Fri Jun 22 00:00:00 UTC 2018

Calmodulin shuttling mediates cytonuclear signaling to trigger experience-dependent transcription and memory.

Nature communications

Cohen SM,Suutari B,He X,Wang Y,Sanchez S,Tirko NN,Mandelberg NJ,Mullins C,Zhou G,Wang S,Kats I,Salah A,Tsien RW,Ma H

Fri Jun 22 00:00:00 UTC 2018

Nogo-A regulates spatial learning as well as memory formation and modulates structural plasticity in the adult mouse hippocampus.

Neurobiology of learning and memory

Zagrebelsky M,Lonnemann N,Fricke S,Kellner Y,Preuß E,Michaelsen-Preusse K,Korte M

137300 was used in immunohistochemistry to examine the contribution of Nogo-A signaling to learning

Wed Feb 01 00:00:00 UTC 2017

Synaptic GAP and GEF Complexes Cluster Proteins Essential for GTP Signaling.

Scientific reports

Wilkinson B,Li J,Coba MP

13-7300 was used in Western Blotting to characterise the interactome of three interacting GAP/GEF proteins - Syngap1, Agap2 and Kalirin - at the postsynaptic density.

Thu Jul 13 00:00:00 UTC 2017

Dopamine neuronal loss contributes to memory and reward dysfunction in a model of Alzheimer's disease.

Nature communications

Nobili A,Latagliata EC,Viscomi MT,Cavallucci V,Cutuli D,Giacovazzo G,Krashia P,Rizzo FR,Marino R,Federici M,De Bartolo P,Aversa D,Dell'Acqua MC,Cordella A,Sancandi M,Keller F,Petrosini L,Puglisi-Allegra S,Mercuri NB,Coccurello R,Berretta N,D'Amelio M

13-7300 was used in Western Blotting to conclude that in this mouse model of AD, degeneration of VTA DAergic neurons at pre-plaque stages contributes to memory deficits and dysfunction of reward processing.

Mon Apr 03 00:00:00 UTC 2017

Chronic Hypobaric Hypoxia Induces Right Ventricular Hypertrophy and Apoptosis in Rats: Therapeutic Potential of Nanocurcumin in Improving Adaptation.

High altitude medicine & biology

Nehra S,Bhardwaj V,Kar S,Saraswat D

13-7300 was used in Western Blotting to suggest the therapeutic potential of nanocurcumin for right heart pathology associated with high altitudes and hypoxic conditions in rodent model.

Thu Dec 01 00:00:00 UTC 2016

Evolutionary and functional perspectives on signaling from neuronal surface to nucleus.

Biochemical and biophysical research communications

Cohen SM,Li B,Tsien RW,Ma H

13-7300 was used in immunocytochemistry to review the mechanism of a gammaCaMKII-CaM nuclear translocation.

Fri Apr 24 00:00:00 UTC 2015

Melatonin stimulates dendrite formation and complexity in the hilar zone of the rat hippocampus: participation of the Ca++/Calmodulin complex.

International journal of molecular sciences

Domínguez-Alonso A,Valdés-Tovar M,Solís-Chagoyán H,Benítez-King G

13-7300 was used in western blot to study the effect of melatonin on dendrite formation in the hilar zone of the rat hippocampus

Fri Jan 16 00:00:00 UTC 2015

miR-26a and miR-384-5p are required for LTP maintenance and spine enlargement.

Nature communications

Gu QH,Yu D,Hu Z,Liu X,Yang Y,Luo Y,Zhu J,Li Z

13-7300 was used in immunocytochemistry to study the roles of miR-26a and miR-384-5p in long-term potentiation

Fri Apr 10 00:00:00 UTC 2015

The schizophrenia susceptibility gene dysbindin regulates dendritic spine dynamics.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Jia JM,Hu Z,Nordman J,Li Z

13-7300 was used in immunocytochemistry and western blot to study the role of dysbindin in the regulation of dendritic spine dynamics

Wed Oct 08 00:00:00 UTC 2014

Calcium and calmodulin-dependent serine/threonine protein kinase type II (CaMKII)-mediated intramolecular opening of integrin cytoplasmic domain-associated protein-1 (ICAP-1α) negatively regulates β1 integrins.

The Journal of biological chemistry

Millon-Frémillon A,Brunner M,Abed N,Collomb E,Ribba AS,Block MR,Albigès-Rizo C,Bouvard D

Fri Jul 12 00:00:00 UTC 2013

The Journal of biological chemistry

Millon-Frémillon A,Brunner M,Abed N,Collomb E,Ribba AS,Block MR,Albigès-Rizo C,Bouvard D

Fri Jul 12 00:00:00 UTC 2013

The Journal of biological chemistry

Millon-Frémillon A,Brunner M,Abed N,Collomb E,Ribba AS,Block MR,Albigès-Rizo C,Bouvard D

Fri Jul 12 00:00:00 UTC 2013

The Journal of biological chemistry

Millon-Frémillon A,Brunner M,Abed N,Collomb E,Ribba AS,Block MR,Albigès-Rizo C,Bouvard D

Fri Jul 12 00:00:00 UTC 2013

Dual regulation of miRNA biogenesis generates target specificity in neurotrophin-induced protein synthesis.

Cell

Huang YW,Ruiz CR,Eyler EC,Lin K,Meffert MK

13-7300 was used in western blot to investigate how BDNF selectively affects mRNA expression.

Fri Mar 02 00:00:00 UTC 2012

Delayed treatment with a novel neurotrophic compound reduces behavioral deficits in rabbit ischemic stroke.

Journal of neurochemistry

Lapchak PA,Schubert DR,Maher PA

13-7300 was used in western blot to study the efficacy and mechanism of CNB-00 in a rabbit ischemic stroke model.

Sat Jan 01 00:00:00 UTC 2011

Stimulation-mediated translocation of calmodulin and neurogranin from soma to dendrites of mouse hippocampal CA1 pyramidal neurons.

Neuroscience

Huang KP,Huang FL,Shetty PK

13-7300 was used in western blot to investigate the association of calmodulin and neurogranin in neurons.

Thu Mar 31 00:00:00 UTC 2011

Stimulation-mediated translocation of calmodulin and neurogranin from soma to dendrites of mouse hippocampal CA1 pyramidal neurons.

Neuroscience

Huang KP,Huang FL,Shetty PK

13-7300 was used in western blot to investigate the association of calmodulin and neurogranin in neurons.

Thu Mar 31 00:00:00 UTC 2011

Netrin-1 induces axon branching in developing cortical neurons by frequency-dependent calcium signaling pathways.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Tang F,Kalil K

Wed Jul 13 00:00:00 UTC 2005

Calcium-calmodulin-dependent protein kinase II contributes to spinal cord central sensitization.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Fang L,Wu J,Lin Q,Willis WD

Wed May 15 00:00:00 UTC 2002

Calcium-calmodulin-dependent protein kinase II contributes to spinal cord central sensitization.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Fang L,Wu J,Lin Q,Willis WD

Wed May 15 00:00:00 UTC 2002

PSD-95 assembles a ternary complex with the N-methyl-D-aspartic acid receptor and a bivalent neuronal NO synthase PDZ domain.

The Journal of biological chemistry

Christopherson KS,Hillier BJ,Lim WA,Bredt DS

Fri Sep 24 00:00:00 UTC 1999

Bioinformatics

Protein Aliases: alpha CaM kinase II; alpha-CaMKII; Ca2+/calmodulin-dependent protein kinase II; Ca2+/calmodulin-dependent protein kinase II alpha; Calci; calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha; calcium/calmodulin-dependent protein kinase II alpha subunit; calcium/calmodulin-dependent protein kinase II alpha-B subunit; calcium/calmodulin-dependent protein kinase type II alpha chain; Calcium/calmodulin-dependent protein kinase type II subunit alpha; CaM kinase II alpha subunit; CaM kinase II subunit alpha; CaM-kinase II alpha chain; CaMK II; CaMK-II alpha subunit; caMK-II subunit alpha; CaMKIINalpha; EC 2.7.11.17; OTTHUMP00000165787; OTTHUMP00000165788; Phospho-CaMKI

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Gene Aliases: CAMK2; CAMK2A; CAMKA; CaMKII; KIAA0968; mKIAA0968; PK2CDD; PKCCD; R74975

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UniProt ID: (Human) Q9UQM7, (Mouse) P11798, (Rat) P11275

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Entrez Gene ID: (Human) 815, (Sheep) 443453, (Mouse) 12322, (Rat) 25400

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Function(s)

protein serine/threonine kinase activity calmodulin-dependent protein kinase activity Ras guanyl-nucleotide exchange factor activity protein binding calmodulin binding ATP binding kinase activity glutamate receptor binding protein homodimerization activity nucleotide binding protein kinase activity transferase activity transferase activity, transferring phosphorus-containing groups GTPase activating protein binding

Process(es)

G1/S transition of mitotic cell cycle response to ischemia protein phosphorylation calcium ion transport positive regulation of cardiac muscle cell apoptotic process phosphorylation peptidyl-serine phosphorylation ionotropic glutamate receptor signaling pathway angiotensin-activated signaling pathway protein autophosphorylation regulation of neurotransmitter secretion regulation of neuronal synaptic plasticity positive regulation of NF-kappaB transcription factor activity positive regulation of calcium ion transport regulation of mitochondrial membrane permeability involved in apoptotic process MAPK cascade Wnt signaling pathway, calcium modulating pathway positive regulation of GTPase activity interferon-gamma-mediated signaling pathway regulation of cellular response to heat

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CaMKII alpha Monoclonal Antibody (Cba-2)

CaMKII alpha Antibody (13-7300) in WB

Product Details

13-7300

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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