Invitrogen

CaMKII alpha/beta Monoclonal Antibody (22B1), FITC

View all (5) CaMKII alpha/beta antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA5-45147

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100
-

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:5,000
-

Immunocytochemistry (ICC/IF)

1:1,000
-

ELISA (ELISA)

Assay-dependent
-

Immunoprecipitation (IP)

Assay-dependent
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

22B1

Immunogen

Synthetic peptide

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

9.1mM sodium bicarbonate/PBS, pH 7.2, with 640.91mM DMSO, 136.36mM ethanolamine

Contains

no preservative

Storage conditions

4°C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2931601

Product Specific Information

1 µg/mL was sufficient for detection of 0.2 µg CamKII by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.|Detects phosphorylated CaMKII from rat tissues. This antibody is specific for alpha and beta subunits of CaMKII only when they are phosphorylated at Thr-286/287 (in beta ).

Target Information

Ca2+/Calmodulin-Dependent Protein Kinase II (CaM Kinase II) is a multi-functional calcium and calmodulin-dependent protein kinase that mediates cellular response to a wide variety of intercellular signals (Kennedy, 1998; Schulman and Hanson, 1993). CaM Kinase II has been shown to regulate diverse cellular functions including synaptic plasticity, neurotransmitter synthesis and release, gene expression, ion channel function, carbohydrate metabolism, cytoskeletal function, and Ca2+-homeostasis (Gleason et al., 2003; Soderling, 2000; Hudmon and Schulman, 2002). Phosphorylation of Thr286 on the kinase produces an autonomously active form of CaM Kinase II (Meng et al., 2003; Picciotto et al., 1993). Autophosphorylation of Thr305 inhibits the activity CaM Kinase II. Phosphorylation at this site appears to reduce the association of CaM Kinase II with the PSD and reduces LTP and learning (Elgersma et al., 2002).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: alpha CaM kinase II; alpha-CaMKII; Ca++/calmodulin-dependent protein kinase II beta subunit; Ca2+/calmodulin-dependent protein kinase II alpha; calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha; calcium/calmodulin-dependent protein kinase (CaM kinase) II beta; calcium/calmodulin-dependent protein kinase II alpha subunit; calcium/calmodulin-dependent protein kinase II alpha-B subunit; calcium/calmodulin-dependent protein kinase II beta; calcium/calmodulin-dependent protein kinase type II alpha chain; calcium/calmodulin-dependent protein kinase type II beta chain; Calcium/calmodulin-dependent protein kinase type II subunit alpha; Calcium/calmodulin-dependent protein kinase type II subunit beta; CaM kinase II alpha subunit; CaM kinase II beta subunit; CaM kinase II subunit alpha; CaM kinase II subunit beta; CaM-kinase II alpha chain; CaM-kinase II beta chain; CaMK II; CaMK-II alpha subunit; caMK-II subunit alpha; caMK-II subunit beta; CaMKIINalpha; proline rich calmodulin-dependent protein kinase

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Gene Aliases: CAM2; CAMK2; CAMK2A; CAMK2B; Camk2d; CAMKA; CAMKB; CaMKII; Ck2b; KIAA0968; mKIAA0968; PK2CDD; PKCCD; R74975

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UniProt ID: (Human) Q9UQM7, (Human) Q13554, (Mouse) P11798, (Rat) P11275, (Rat) P08413, (Mouse) P28652

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Entrez Gene ID: (Human) 815, (Human) 816, (Mouse) 12322, (Rat) 25400, (Rat) 24245, (Mouse) 12323

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Function(s)
protein serine/threonine kinase activity calmodulin-dependent protein kinase activity Ras guanyl-nucleotide exchange factor activity protein binding calmodulin binding ATP binding kinase activity glutamate receptor binding protein homodimerization activity actin binding protein kinase binding phospholipase binding nucleotide binding protein kinase activity transferase activity transferase activity, transferring phosphorus-containing groups GTPase activating protein binding
Process(es)
MAPK cascade protein phosphorylation signal transduction positive regulation of neuron projection development regulation of skeletal muscle adaptation positive regulation of phospholipase A2 activity positive regulation of GTPase activity protein autophosphorylation regulation of long-term neuronal synaptic plasticity cell projection morphogenesis regulation of synapse structural plasticity regulation of calcium ion transport interferon-gamma-mediated signaling pathway regulation of dendritic spine development positive regulation of dendritic spine morphogenesis positive regulation of synapse maturation regulation of cellular response to heat G1/S transition of mitotic cell cycle response to ischemia calcium ion transport positive regulation of cardiac muscle cell apoptotic process phosphorylation peptidyl-serine phosphorylation ionotropic glutamate receptor signaling pathway angiotensin-activated signaling pathway regulation of neurotransmitter secretion regulation of neuronal synaptic plasticity positive regulation of NF-kappaB transcription factor activity positive regulation of calcium ion transport regulation of mitochondrial membrane permeability involved in apoptotic process Wnt signaling pathway, calcium modulating pathway inhibitory G-protein coupled receptor phosphorylation regulation of synaptic transmission, cholinergic response to cadmium ion neuromuscular process controlling balance long-term synaptic potentiation activation of meiosis involved in egg activation positive regulation of apoptotic signaling pathway nervous system development cell differentiation
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