Invitrogen

CaMKII beta Monoclonal Antibody (CB-beta-1)

CaMKII beta Antibody in Western Blot (WB)

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CaMKII beta Antibody (13-9800) in WB

Western blot analysis was performed on whole cell extracts (30 µg lysate) of SH-SY5Y (Lane 1), U-87MG (Lane 2) and PC12 (Lane 3). The blots were probed with Anti-CaM Kinase II beta Mouse Monoclonal Antibody (Product # 13-9800, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP conjugate (Product # 62-6520, 1:4000 dilution). A 70 kDa band corresponding to CaM Kinase II beta was observed only in PC12. As it is a Rat immunogen, it did not react with human cell lines. Known quantity of pr... View More

CaMKII beta Antibody in Immunoprecipitation (IP)

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Product Details

13-9800

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 25 publications 25 publications

Immunohistochemistry (IHC)

Assay-dependent

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

View 5 publications 5 publications

ELISA (ELISA)

Assay-dependent

Immunoprecipitation (IP)

Assay-dependent

View 2 publications 2 publications

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Fish, Human, Mouse, Sheep, Rat, Sea urchin

Published species

Human, Mouse, Non-human primate, Rat

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

CB-beta-1

Immunogen

Purified rat brain CaM Kinase.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533045

Target Information

CAMK2B (CaMKII beta) is a serine/threonine kinase that plays a role in synaptic plasticity, synaptic vesicle mobilization and regulation of gene expression. As a dominant kinase in the central nervous system it may function in long term potentiation and neurotransmitter release. There are four CaMKII isozymes, designated alpha, beta, gamma and delta, which may or may not be co-expressed in the same tissue type. CaMKII alpha is autophosphorylated on Thr 286 upon the binding of the Ca2+/CaM complex to the autoinhibitory domain of CaMKII. This process is called Ca2+/CaM trapping, which is thought to be involved in the synaptic encoding of information.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Targeting the interaction of GABA_B receptors with CaMKII with an interfering peptide restores receptor expression after cerebral ischemia and inhibits progressive neuronal death in mouse brain cells and slices.

Brain pathology (Zurich, Switzerland)

Balakrishnan K,Hleihil M,Bhat MA,Ganley RP,Vaas M,Klohs J,Zeilhofer HU,Benke D

13-9800 was used in Western Blotting to develop R1-Pep that inhibits CaMKII activity to restore cell cell surface expression and function of GABAB receptors, providing neuroprotection to inhibit progressive neuronal death after ischemic stroke.

Sun Jan 01 00:00:00 UTC 2023

Loss of CAMK2G affects intrinsic and motor behavior but has minimal impact on cognitive behavior.

Frontiers in neuroscience

Rigter PMF,de Konink C,van Woerden GM

13-9800 was used in Western Blotting to provide evidence for a unique function of this specific CAMK2 isozyme in the brain and further support the role of CAMK2G in neurodevelopment.

Wed Sep 11 00:00:00 UTC 2024

Loss of CAMK2G affects intrinsic and motor behavior but has minimal impact on cognitive behavior.

Frontiers in neuroscience

Rigter PMF,de Konink C,van Woerden GM

13-9800 was used in Western Blotting to provide evidence for a unique function of this specific CAMK2 isozyme in the brain and further support the role of CAMK2G in neurodevelopment.

Wed Sep 11 00:00:00 UTC 2024

Adult Camk2a gene reinstatement restores the learning and plasticity deficits of Camk2a knockout mice.

iScience

Rigter PMF,Wallaard I,Aghadavoud Jolfaei M,Kingma J,Post L,Elgersma M,Elgersma Y,van Woerden GM

13-9800 was used in Western Blotting to investigate the critical developmental time window in which calcium/calmodulin-dependent protein kinase II (CAMK2A) needs to be expressed for normal brain development and whether expression at later stages is beneficial to restore normal functioning using an inducible Camk2a mouse model.

Fri Nov 18 00:00:00 UTC 2022

Adult Camk2a gene reinstatement restores the learning and plasticity deficits of Camk2a knockout mice.

iScience

Rigter PMF,Wallaard I,Aghadavoud Jolfaei M,Kingma J,Post L,Elgersma M,Elgersma Y,van Woerden GM

Fri Nov 18 00:00:00 UTC 2022

Herpes Simplex Virus Type 1 Neuronal Infection Triggers the Disassembly of Key Structural Components of Dendritic Spines.

Frontiers in cellular neuroscience

Acuña-Hinrichsen F,Covarrubias-Pinto A,Ishizuka Y,Stolzenbach MF,Martin C,Salazar P,Castro MA,Bramham CR,Otth C

13-9800 was used in Immunocytochemistry to demonstrate that HSV-1 infection induces structural disassembly and functional deregulation in cultured cortical neurons, an altered glutamate response, Arc accumulation within the somata, and decreased expression of spine scaffolding-like proteins such as PSD-95, Drebrin and CaMKIIβ.

Sat Nov 11 00:00:00 UTC 2023

Herpes Simplex Virus Type 1 Neuronal Infection Triggers the Disassembly of Key Structural Components of Dendritic Spines.

Frontiers in cellular neuroscience

Acuña-Hinrichsen F,Covarrubias-Pinto A,Ishizuka Y,Stolzenbach MF,Martin C,Salazar P,Castro MA,Bramham CR,Otth C

Sat Nov 11 00:00:00 UTC 2023

Gene-environment interactions mediate stress susceptibility and resilience through the CaMKIIβ/TARPγ-8/AMPAR pathway.

iScience

Sakai Y,Li H,Inaba H,Funayama Y,Ishimori E,Kawatake-Kuno A,Yamagata H,Seki T,Hobara T,Nakagawa S,Watanabe Y,Tomita S,Murai T,Uchida S

13-9800 was used in Western Blot to elucidate the mechanisms of ventral hippocampus and gene-environment cross-talk implicated in the pathophysiology of depression.

Fri May 21 00:00:00 UTC 2021

Gene-environment interactions mediate stress susceptibility and resilience through the CaMKIIβ/TARPγ-8/AMPAR pathway.

iScience

Sakai Y,Li H,Inaba H,Funayama Y,Ishimori E,Kawatake-Kuno A,Yamagata H,Seki T,Hobara T,Nakagawa S,Watanabe Y,Tomita S,Murai T,Uchida S

13-9800 was used in Western Blot to elucidate the mechanisms of ventral hippocampus and gene-environment cross-talk implicated in the pathophysiology of depression.

Fri May 21 00:00:00 UTC 2021

Molecular motor protein KIF5C mediates structural plasticity and long-term memory by constraining local translation.

Cell reports

Swarnkar S,Avchalumov Y,Espadas I,Grinman E,Liu XA,Raveendra BL,Zucca A,Mediouni S,Sadhu A,Valente S,Page D,Miller K,Puthanveettil SV

13-9800 was used in ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot to explore the roles of KIF5C and KIF3A, two members of kinesin superfamily of molecular motors (Kifs), and find that loss of function of either kinesin decreases dendritic arborization and spine density whereas gain of function of KIF5C enhances it.

Tue Jul 13 00:00:00 UTC 2021

Gating of hippocampal rhythms and memory by synaptic plasticity in inhibitory interneurons.

Neuron

He X,Li J,Zhou G,Yang J,McKenzie S,Li Y,Li W,Yu J,Wang Y,Qu J,Wu Z,Hu H,Duan S,Ma H

13-9800 was used in Western Blotting to suggest that E→I synaptic plasticity, exemplified by LTPE→I, plays a gatekeeping role in tuning experience-dependent brain rhythms and mnemonic function.

Wed Mar 17 00:00:00 UTC 2021

A simple DMSO-based method for cryopreservation of primary hippocampal and cortical neurons.

Journal of neuroscience methods

Ishizuka Y,Bramham CR

13-9800 was used in Western Blot to present a simplified cryopreservation method for rodent primary hippocampal and cortical neurons from embryonic day 18.5 fetuses, using DMSO-containing traditional cell freezing medium.

Sun Mar 01 00:00:00 UTC 2020

CAMK2-Dependent Signaling in Neurons Is Essential for Survival.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Kool MJ,Proietti Onori M,Borgesius NZ,van de Bree JE,Elgersma-Hooisma M,Nio E,Bezstarosti K,Buitendijk GHS,Aghadavoud Jolfaei M,Demmers JAA,Elgersma Y,van Woerden GM

Published figure using CaMKII beta monoclonal antibody (Product # 13-9800) in Western Blot

Wed Jul 10 00:00:00 UTC 2019

A novel role for CAMKIIβ in the regulation of cortical neuron migration: implications for neurodevelopmental disorders.

Molecular psychiatry

Nicole O,Bell DM,Leste-Lasserre T,Doat H,Guillemot F,Pacary E

13-9800 was used in Western Blotting to determine the specific function of CamKIIB in the brain.

Thu Nov 01 00:00:00 UTC 2018

Cyclophilin D regulates neuronal activity-induced filopodiagenesis by fine-tuning dendritic mitochondrial calcium dynamics.

Journal of neurochemistry

Sui S,Tian J,Gauba E,Wang Q,Guo L,Du H

13-9800 was used in Western Blotting to reveal the physiological function of CypD in dendritic plasticity by acting as a fine-tuner of mitochondrial calcium homeostasis.

Wed Aug 01 00:00:00 UTC 2018

CaMKIIβ is localized in dendritic spines as both drebrin-dependent and drebrin-independent pools.

Journal of neurochemistry

Yamazaki H,Sasagawa Y,Yamamoto H,Bito H,Shirao T

13-9800 was used in Immunocytochemistry to study the beta subunit of Ca2+/calmodulin-dependent protein kinase II as a drebrin-binding protein, and investigate the drebrin-CaMKIIΒ relationship in dendritic spines using rat hippocampal neurons.

Sun Jul 01 00:00:00 UTC 2018

Differential Involvement of Kinase Activity of Ca²⁺/Calmodulin-Dependent Protein Kinase IIα in Hippocampus- and Amygdala-Dependent Memory Revealed by Kinase-Dead Knock-In Mouse.

eNeuro

Yamagata Y,Yanagawa Y,Imoto K

13-9800 was used in Western Blotting to indicate that kinase activity of CaMKIIα is indispensable for hippocampus-dependent memory, but not necessarily for amygdala-dependent memory.

Wed Feb 27 00:00:00 UTC 2019

Reciprocal control of excitatory synapse numbers by Wnt and Wnt inhibitor PRR7 secreted on exosomes.

Nature communications

Lee SH,Shin SM,Zhong P,Kim HT,Kim DI,Kim JM,Heo WD,Kim DW,Yeo CY,Kim CH,Liu QS

Fri Aug 24 00:00:00 UTC 2018

Calmodulin shuttling mediates cytonuclear signaling to trigger experience-dependent transcription and memory.

Nature communications

Cohen SM,Suutari B,He X,Wang Y,Sanchez S,Tirko NN,Mandelberg NJ,Mullins C,Zhou G,Wang S,Kats I,Salah A,Tsien RW,Ma H

13-9800 was used in Western Blotting to show that elimination of γCaMKII prevents activity-dependent expression of key genes (BDNF, c-Fos, Arc), inhibits persistent synaptic strengthening, and impairs spatial memory in vivo.

Fri Jun 22 00:00:00 UTC 2018

Regulation of spinogenesis in mature Purkinje cells via mGluR/PKC-mediated phosphorylation of CaMKIIβ.

Proceedings of the National Academy of Sciences of the United States of America

Sugawara T,Hisatsune C,Miyamoto H,Ogawa N,Mikoshiba K

13-9800 was used in Immunohistochemistry-immunofluorescence to suggest that phosphorylation of CaMKIIΒ through the mGluR/IP3R1/PKC signalling pathway represses excessive spine formation and elongation in mature Purkinje cells.

Tue Jun 27 00:00:00 UTC 2017

Regulation of spinogenesis in mature Purkinje cells via mGluR/PKC-mediated phosphorylation of CaMKIIβ.

Proceedings of the National Academy of Sciences of the United States of America

Sugawara T,Hisatsune C,Miyamoto H,Ogawa N,Mikoshiba K

Tue Jun 27 00:00:00 UTC 2017

Regulation of spinogenesis in mature Purkinje cells via mGluR/PKC-mediated phosphorylation of CaMKIIβ.

Proceedings of the National Academy of Sciences of the United States of America

Sugawara T,Hisatsune C,Miyamoto H,Ogawa N,Mikoshiba K

Tue Jun 27 00:00:00 UTC 2017

Regulation of spinogenesis in mature Purkinje cells via mGluR/PKC-mediated phosphorylation of CaMKIIβ.

Proceedings of the National Academy of Sciences of the United States of America

Sugawara T,Hisatsune C,Miyamoto H,Ogawa N,Mikoshiba K

Tue Jun 27 00:00:00 UTC 2017

A Novel Human CAMK2A Mutation Disrupts Dendritic Morphology and Synaptic Transmission, and Causes ASD-Related Behaviors.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Stephenson JR,Wang X,Perfitt TL,Parrish WP,Shonesy BC,Marks CR,Mortlock DP,Nakagawa T,Sutcliffe JS,Colbran RJ

13-9800 was used in Western Blotting to demonstrate that this ASD-linked de novo CAMK2A mutation disrupts multiple CaMKII functions, induces synaptic deficits, and causes ASD-related behavioral alterations.

Wed Feb 22 00:00:00 UTC 2017

Dynamic Arc SUMOylation and Selective Interaction with F-Actin-Binding Protein Drebrin A in LTP Consolidation In Vivo.

Frontiers in synaptic neuroscience

Nair RR,Patil S,Tiron A,Kanhema T,Panja D,Schiro L,Parobczak K,Wilczynski G,Bramham CR

Published figure using CaMKII beta monoclonal antibody (Product # 13-9800) in Immunoprecipitation

Tue Sep 29 00:00:00 UTC 2020

The molecular, temporal and region-specific requirements of the beta isoform of Calcium/Calmodulin-dependent protein kinase type 2 (CAMK2B) in mouse locomotion.

Scientific reports

Kool MJ,van de Bree JE,Bodde HE,Elgersma Y,van Woerden GM

13-9800 was used in western blot investigate the molecular, temporal, and brain region-specific requirements of CAMK2B for locomotion

Tue May 31 00:00:00 UTC 2016

Electron tomographic structure and protein composition of isolated rat cerebellar, hippocampal and cortical postsynaptic densities.

Neuroscience

Farley MM,Swulius MT,Waxham MN

13-9800 was used in western blot to characterize rat cerebellar, hippocampal and cortical postsynaptic densities

Thu Sep 24 00:00:00 UTC 2015

Evolutionary and functional perspectives on signaling from neuronal surface to nucleus.

Biochemical and biophysical research communications

Cohen SM,Li B,Tsien RW,Ma H

13-9800 was used in immunocytochemistry to review the mechanism of a gammaCaMKII-CaM nuclear translocation.

Fri Apr 24 00:00:00 UTC 2015

Targeting the CaMKII/ERK Interaction in the Heart Prevents Cardiac Hypertrophy.

PloS one

Cipolletta E,Rusciano MR,Maione AS,Santulli G,Sorriento D,Del Giudice C,Ciccarelli M,Franco A,Crola C,Campiglia P,Sala M,Gomez-Monterrey I,De Luca N,Trimarco B,Iaccarino G,Illario M

13-9800 was used in western blot to test if CaMKII association promotes cardiac hypertrophy through ERK nuclear localization.

Fri Mar 25 00:00:00 UTC 2016

CaMKII regulates diacylglycerol lipase-α and striatal endocannabinoid signaling.

Nature neuroscience

Shonesy BC,Wang X,Rose KL,Ramikie TS,Cavener VS,Rentz T,Baucum AJ,Jalan-Sakrikar N,Mackie K,Winder DG,Patel S,Colbran RJ

13-9800 was used in western blot to investigate the mechanisms that regulate striatal endocannabinoid signaling

Mon Apr 01 00:00:00 UTC 2013

GluN2B subunit deletion reveals key role in acute and chronic ethanol sensitivity of glutamate synapses in bed nucleus of the stria terminalis.

Proceedings of the National Academy of Sciences of the United States of America

Wills TA,Klug JR,Silberman Y,Baucum AJ,Weitlauf C,Colbran RJ,Delpire E,Winder DG

13-9800 was used in Western Blotting to demonstrate that GluN2B is a key point of regulation for ethanol's actions and suggest a unique role of extrasynaptic GluN2B-containing receptors in facilitating LTP.

Tue Jan 31 00:00:00 UTC 2012

Electron cryotomography of postsynaptic densities during development reveals a mechanism of assembly.

Neuroscience

Swulius MT,Farley MM,Bryant MA,Waxham MN

13-9800 was used in immunohistochemistry to elucidate the 3D structure of postsynaptic densities.

Thu Jun 14 00:00:00 UTC 2012

Loss of GluN2B-containing NMDA receptors in CA1 hippocampus and cortex impairs long-term depression, reduces dendritic spine density, and disrupts learning.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Brigman JL,Wright T,Talani G,Prasad-Mulcare S,Jinde S,Seabold GK,Mathur P,Davis MI,Bock R,Gustin RM,Colbran RJ,Alvarez VA,Nakazawa K,Delpire E,Lovinger DM,Holmes A

13-9800 was used in western blot to characterize mice lacking GluN2B in the pyramidal neurons of the cortex and CA1 subregion of the hippocampus

Wed Mar 31 00:00:00 UTC 2010

Structure and composition of the postsynaptic density during development.

The Journal of comparative neurology

Swulius MT,Kubota Y,Forest A,Waxham MN

13-9800 was used in immunocytochemistry and western blot to study the structure and composition of post-synaptic densities during development

Fri Oct 15 00:00:00 UTC 2010

Identification and validation of novel spinophilin-associated proteins in rodent striatum using an enhanced ex vivo shotgun proteomics approach.

Molecular & cellular proteomics : MCP

Baucum AJ,Jalan-Sakrikar N,Jiao Y,Gustin RM,Carmody LC,Tabb DL,Ham AJ,Colbran RJ

13-9800 was used in western blot to investigate the roles of spinophilin in mature brain

Tue Jun 01 00:00:00 UTC 2010

Interactions of the NPXY microdomains of the low density lipoprotein receptor-related protein 1.

Proteomics

Guttman M,Betts GN,Barnes H,Ghassemian M,van der Geer P,Komives EA

13-9800 was used in western blot to identify proteins that bind phosphorylated and unphosphorylated LRP1

Sun Nov 01 00:00:00 UTC 2009

Inhibitory autophosphorylation of CaMKII controls PSD association, plasticity, and learning.

Neuron

Elgersma Y,Fedorov NB,Ikonen S,Choi ES,Elgersma M,Carvalho OM,Giese KP,Silva AJ

Thu Oct 24 00:00:00 UTC 2002

Inositol 1,4,5-trisphosphate 3-kinase A associates with F-actin and dendritic spines via its N terminus.