Invitrogen

CaMKII delta Polyclonal Antibody

5 Published Figures

9 References

View all (18) CaMKII delta antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (10)
Published Figures (5)

CaMKII delta Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 15

CaMKII delta Antibody (PA5-22168) in ICC/IF

Immunofluorescence analysis of CamKII delta was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CaMKII delta Polyclonal Antibody (Product # PA5-22168) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsiu... View More

CaMKII delta Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CaMKII delta Antibody in Western Blot (WB)

CaMKII delta Antibody in Immunoprecipitation (IP)

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Product Details

PA5-22168

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:50,000

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

Immunoprecipitation (IP)

1:100-1:500

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rabbit, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human CaMKII delta. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.62 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_11153337

Product Specific Information

Recommended positive controls: 293T, A431, HeLa, HepG2, A549, H1299, HCT116, mouse brain, rat brain.

Predicted reactivity: Mouse (100%), Rat (80%), Xenopus laevis (94%), Pig (100%), Rabbit (99%), Chicken (98%), Bovine (92%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

CaMKII delta belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. is a delta chain. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Distinct isoforms of this chain have different expression patterns.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

CaMKII and reactive oxygen species contribute to early reperfusion arrhythmias, but oxidation of CaMKIIδ at methionines 281/282 is not a determining factor.

Journal of molecular and cellular cardiology

Haugsten Hansen M,Sadredini M,Hasic A,Anderson ME,Sjaastad I,Korseberg Stokke M

PA5-22168 was used in Western Blotting to investigate the role of oxidized-CaMKIIδ (Ox-CaMKIIδ) in early ischemia-reperfusion arrhythmias (IRA).

Wed Feb 01 00:00:00 UTC 2023

Detrimental proarrhythmogenic interaction of Ca²⁺/calmodulin-dependent protein kinase II and Na_V1.8 in heart failure.

Nature communications

Bengel P,Dybkova N,Tirilomis P,Ahmad S,Hartmann N,A Mohamed B,Krekeler MC,Maurer W,Pabel S,Trum M,Mustroph J,Gummert J,Milting H,Wagner S,Ljubojevic-Holzer S,Toischer K,Maier LS,Hasenfuss G,Streckfuss-Bömeke K,Sossalla S

PA5-22168 was used in Western Blot to elucidate the role of the sodium channel isoform NaV1.8 for CaMKIIδc-dependent proarrhythmia.

Mon Nov 15 00:00:00 UTC 2021

Detrimental proarrhythmogenic interaction of Ca²⁺/calmodulin-dependent protein kinase II and Na_V1.8 in heart failure.

Nature communications

PA5-22168 was used in Western Blot to elucidate the role of the sodium channel isoform NaV1.8 for CaMKIIδc-dependent proarrhythmia.

Mon Nov 15 00:00:00 UTC 2021

Detrimental proarrhythmogenic interaction of Ca²⁺/calmodulin-dependent protein kinase II and Na_V1.8 in heart failure.

Nature communications

PA5-22168 was used in Western Blot to elucidate the role of the sodium channel isoform NaV1.8 for CaMKIIδc-dependent proarrhythmia.

Mon Nov 15 00:00:00 UTC 2021

The oxidation-resistant CaMKII-MM281/282VV mutation does not prevent arrhythmias in CPVT1.

Physiological reports

Sadredini M,Manotheepan R,Lehnart SE,Anderson ME,Sjaastad I,Stokke MK

PA5-22168 was used in Western Blotting to test whether an oxidation-resistant form of CaMKII protects mice carrying the CPVT1-causative mutation RyR2-R2474S (RyR2-RS) against arrhythmias.

Wed Sep 01 00:00:00 UTC 2021

Gating of hippocampal rhythms and memory by synaptic plasticity in inhibitory interneurons.

Neuron

He X,Li J,Zhou G,Yang J,McKenzie S,Li Y,Li W,Yu J,Wang Y,Qu J,Wu Z,Hu H,Duan S,Ma H

PA5-22168 was used in Western Blotting to suggest that E→I synaptic plasticity, exemplified by LTPE→I, plays a gatekeeping role in tuning experience-dependent brain rhythms and mnemonic function.

Wed Mar 17 00:00:00 UTC 2021

Long-term effects of empagliflozin on excitation-contraction-coupling in human induced pluripotent stem cell cardiomyocytes.

Journal of molecular medicine (Berlin, Germany)

Pabel S,Reetz F,Dybkova N,Shomroni O,Salinas G,Mustroph J,Hammer KP,Hasenfuss G,Hamdani N,Maier LS,Streckfuss-Bömeke K,Sossalla S

PA5-22168 was used in Western Blotting to investigate the long-term effects (up to 2 months) of empagliflozin on excitation-contraction (EC)-coupling in human cardiomyocytes derived from induced pluripotent stem cells (iPSC-CM) in a blinded manner.

Tue Dec 01 00:00:00 UTC 2020

Long-term effects of empagliflozin on excitation-contraction-coupling in human induced pluripotent stem cell cardiomyocytes.

Journal of molecular medicine (Berlin, Germany)

Pabel S,Reetz F,Dybkova N,Shomroni O,Salinas G,Mustroph J,Hammer KP,Hasenfuss G,Hamdani N,Maier LS,Streckfuss-Bömeke K,Sossalla S

Tue Dec 01 00:00:00 UTC 2020

Inhibition of calcium/calmodulin-dependent kinase II restores contraction and relaxation in isolated cardiac muscle from type 2 diabetic rats.

Cardiovascular diabetology

Daniels LJ,Wallace RS,Nicholson OM,Wilson GA,McDonald FJ,Jones PP,Baldi JC,Lamberts RR,Erickson JR

Published figure using CaMKII delta polyclonal antibody (Product # PA5-22168) in Western Blot

Thu Jun 14 00:00:00 UTC 2018

Arrhythmias precede cardiomyopathy and remodeling of Ca²⁺ handling proteins in a novel model of long QT syndrome.

Journal of molecular and cellular cardiology

Montnach J,Chizelle FF,Belbachir N,Castro C,Li L,Loussouarn G,Toumaniantz G,Carcouët A,Meinzinger AJ,Shmerling D,Benitah JP,Gómez AM,Charpentier F,Baró I

PA5-22168 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to show that the Scn5a+/?QKP mouse model recapitulates the clinical phenotype of mutation carriers and provides new and unexpected insights into the pathological development of the disease in patients carrying the QKP1507-1509 deletion.

Mon Oct 01 00:00:00 UTC 2018

Beta-Adrenoceptor Stimulation Reveals Ca2+ Waves and Sarcoplasmic Reticulum Ca2+ Depletion in Left Ventricular Cardiomyocytes from Post-Infarction Rats with and without Heart Failure.

PloS one

Sadredini M,Danielsen TK,Aronsen JM,Manotheepan R,Hougen K,Sjaastad I,Stokke MK

Published figure using CaMKII delta polyclonal antibody (Product # PA5-22168) in Western Blot

Fri Sep 09 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: Ca++/calmodulin-dependent protein kinase 2 delta subunit; Ca++/calmodulin-dependent protein kinase II delta subunit; Ca++/calmodulin-dependent protein kinase II, delta subunit; calcium/calmodulin-dependent protein kinase (CaM kinase) II delta; calcium/calmodulin-dependent protein kinase II delta; calcium/calmodulin-dependent protein kinase type II delta chain; Calcium/calmodulin-dependent protein kinase type II subunit delta; calmodulin-dependent protein kinase II-delta dash; CaM kinase II delta subunit; CaM kinase II subunit delta; CaM-kinase II delta chain; CAM2; CaMK-II delta subunit; caMK-II subunit delta; CAMK2B; CAMK2G; CAMKB; CAMKG

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Gene Aliases: 2810011D23Rik; 8030469K03Rik; [d]-CaMKII; CaMK II; CAMK1; Camk2; CAMK2D; CAMKD; Camki; Kiaa4163; RATCAMKI

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UniProt ID: (Human) Q13557, (Rat) P15791, (Mouse) Q6PHZ2, (Rabbit) O77708

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Entrez Gene ID: (Human) 817, (Rat) 24246, (Mouse) 108058, (Rabbit) 100101557

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Function(s)

protein serine/threonine kinase activity calmodulin-dependent protein kinase activity Ras guanyl-nucleotide exchange factor activity protein binding calmodulin binding ATP binding sodium channel inhibitor activity titin binding protein homodimerization activity ion channel binding nitric-oxide synthase binding nucleotide binding protein kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups

Process(es)

G1/S transition of mitotic cell cycle MAPK cascade regulation of cell growth response to hypoxia regulation of the force of heart contraction regulation of membrane depolarization regulation of transcription from RNA polymerase II promoter protein phosphorylation calcium ion transport regulation of heart contraction regulation of G2/M transition of mitotic cell cycle positive regulation of cardiac muscle hypertrophy regulation of cell communication by electrical coupling positive regulation of cardiac muscle cell apoptotic process regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion positive regulation of smooth muscle cell migration peptidyl-serine phosphorylation peptidyl-threonine phosphorylation cellular potassium ion homeostasis endoplasmic reticulum calcium ion homeostasis positive regulation of Rac protein signal transduction positive regulation of GTPase activity protein autophosphorylation positive regulation of smooth muscle cell proliferation protein oligomerization relaxation of cardiac muscle regulation of ryanodine-sensitive calcium-release channel activity interferon-gamma-mediated signaling pathway regulation of cellular localization cell growth involved in cardiac muscle cell development positive regulation of ERK1 and ERK2 cascade cellular response to calcium ion cardiac muscle cell contraction regulation of heart rate by cardiac conduction regulation of cardiac muscle cell action potential regulation of cardiac muscle cell action potential involved in regulation of contraction regulation of cellular response to heat regulation of histone deacetylase activity regulation of cell communication by electrical coupling involved in cardiac conduction regulation of relaxation of cardiac muscle negative regulation of sodium ion transmembrane transport regulation of calcium ion transmembrane transport via high voltage-gated calcium channel negative regulation of sodium ion transmembrane transporter activity regulation of sodium ion transport cardiac muscle contraction phosphorylation

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CaMKII delta Polyclonal Antibody

CaMKII delta Antibody (PA5-22168) in ICC/IF

Product Details

PA5-22168

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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