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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • CaV1.1 Antibodies

          Invitrogen

          CaV1.1 Monoclonal Antibody (1A)

          Advanced Verification
          29 Published Figures
          76 References
          View all (10) CaV1.1 antibodies
          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite CaV1.1 Monoclonal Antibody (1A)

          • Antibody Testing Data (2)
          • Published Figures (29)
          • Advanced Verification (1)
          CaV1.1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.
          CaV1.1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 32

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          CaV1.1 Antibody (MA3-920) in IHC (P)

          Immunohistochemistry was performed on normal biopsies of deparaffinized human skeletal muscle tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a Mouse Monocl... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          CaV1.1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CaV1.1 Antibody in Flow Cytometry (Flow)
          CaV1.1 Antibody in Immunohistochemistry (IHC)
          CaV1.1 Antibody in Immunocytochemistry (ICC/IF)
          CaV1.1 Antibody in Immunocytochemistry (ICC/IF)
          CaV1.1 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
          CaV1.1 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          CaV1.1 Antibody in Immunohistochemistry (IHC)
          CaV1.1 Antibody in Immunohistochemistry (IHC)
          CaV1.1 Antibody in Immunohistochemistry (IHC)
          CaV1.1 Antibody in Immunohistochemistry (IHC)
          CaV1.1 Antibody in Western Blot, Immunohistochemistry (WB, IHC)
          CaV1.1 Antibody in Immunohistochemistry (IHC)
          CaV1.1 Antibody in Immunohistochemistry (IHC)
          CaV1.1 Antibody in Immunohistochemistry (IHC)
          CaV1.1 Antibody in Western Blot (WB)
          CaV1.1 Antibody in Western Blot (WB)
          CaV1.1 Antibody in Western Blot (WB)
          CaV1.1 Antibody in Western Blot (WB)
          CaV1.1 Antibody in Western Blot (WB)
          CaV1.1 Antibody in Western Blot (WB)
          CaV1.1 Antibody in Western Blot (WB)
          CaV1.1 Antibody in Western Blot (WB)
          CaV1.1 Antibody in Western Blot (WB)
          CaV1.1 Antibody in Western Blot (WB)
          CaV1.1 Antibody in Western Blot (WB)
          CaV1.1 Antibody in Western Blot (WB)
          CaV1.1 Antibody in Western Blot (WB)
          CaV1.1 Antibody in Western Blot (WB)
          CaV1.1 Antibody in Western Blot (WB)
          CaV1.1 Antibody in Immunomicroscopy (IM)
          CaV1.1 Antibody

          Product Details

          MA3-920

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500
          View 41 publications 41 publications

          Immunohistochemistry (IHC)

          -
          View 20 publications 20 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:20
          -

          Immunohistochemistry (Frozen) (IHC (F))

          1:200
          -

          Immunocytochemistry (ICC/IF)

          -
          View 14 publications 14 publications

          Flow Cytometry (Flow)

          1 µg/test
          -

          Immunoprecipitation (IP)

          Assay-dependent
          -

          Immunomicroscopy (IM)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Guinea pig, Human, Mouse, Rabbit, Rat

          Published species

          Fish, Human, Mouse, Non-human primate, Rabbit, Rat, Zebrafish

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          1A

          Immunogen

          Purified rabbit muscle T-tubule dihydropyridine receptor.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Protein A

          Storage buffer

          PBS, pH 7.4

          Contains

          0.05% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2069575

          Product Specific Information

          MA3-920 detects 1,4-dihydropyridine (DHP) receptor alpha-1 subunit in human, rat, mouse, guinea pig and rabbit skeletal muscle. The DHP Receptor alpha-1 protein is also known as CACNA1S or Cav1.1 alpha-1 subunit.



          MA3-920 has been successfully used in Western blot, FACS, immunohistochemistry and immunoprecipitation procedures. By Western blot, this antibody detects an ~200 kDa protein representing the DHP receptor in rat skeletal muscle extracts. Immunohistochemical staining of DHP receptor in rabbit skeletal muscle with MA3-920 results in double rows of discrete punctate staining representing pairs of triads on the opposing sides of the Z-lines. This product can be used to inhibit the DHP-sensitive calcium current in BC3H1 mouse muscle cells.



          The MA3-920 antigen is purified rabbit muscle T-tubule DHP receptor.

          Target Information

          Voltage-sensitive calcium channels mediate the entry of calcium into many types of excitable cells and thus play a key role in neurotransmitter release and excitation-contraction (E-C) coupling. The 1,4-dihydropyridines (DHPs) are synthetic organic compounds which can be used to identify the L-type calcium channels that are found in all types of vertebrate muscle, neuronal and neuroendocrine cells. The DHP receptor is part of the L-type calcium channel complex and is thought to be the voltage sensor in E-C coupling. The purified DHP receptor isolated from triads is composed of at least four subunits. The alpha-1 subunit contains the binding site for the DHPs and shows high sequence homology to the voltage gated sodium channel. The alpha-2 subunit is a large glycoprotein associated with the DHP receptor which was first described in skeletal muscle and is also found in high concentrations in other excitable tissues such as cardiac muscle and brain and in low concentrations in most other tissues studied. The other two subunits that co-purify with the DHP receptor are termed beta and gamma.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: calcium channel, L type, alpha 1 polypeptide, isoform 3 (skeletal muscle, hypokalemic periodic paralysis); Calcium channel, L type, alpha-1 polypeptide, isoform 3, skeletal muscle; calcium channel, voltage-dependent, L type, alpha 1S subunit; Cav1.1 alpha-1; DHP; DHPR; DHPR alpha1s; Dihydropyridine receptor; dihydropyridine receptor alpha 1 subunit; dihydropyridine receptor alpha 1S; Dihydropyridine receptor alpha-1S subunit; dihydropyridine-sensitive L-type calcium channel alpha-1 subunit; muscle dysgenesis; ROB1; Voltage-dependent L-type calcium channel subunit alpha-1S; Voltage-gated calcium channel subunit alpha Cav1.1

          View more View less

          Gene Aliases: AW493108; CACH1; Cach1b; CACN1; CACNA1S; CACNL1A3; Cav1.1; CCHL1A3; fmd; HOKPP; HOKPP1; hypoPP; mdg; MHS5; sj; TTPP1

          View more View less

          UniProt ID: (Human) Q13698, (Rabbit) P07293, (Rat) Q62817, (Mouse) Q02789

          View more View less

          Entrez Gene ID: (Human) 779, (Rabbit) 100009585, (Rat) 682930, (Mouse) 12292

          View more View less

          Function(s)
          voltage-gated calcium channel activity protein binding high voltage-gated calcium channel activity metal ion binding ion channel activity voltage-gated ion channel activity calcium channel activity
          Process(es)
          skeletal system development ossification extraocular skeletal muscle development calcium ion transport muscle contraction striated muscle contraction endoplasmic reticulum organization skeletal muscle tissue development myoblast fusion neuromuscular junction development regulation of ion transmembrane transport skeletal muscle adaptation skeletal muscle fiber development muscle cell development calcium ion import calcium ion transmembrane transport membrane depolarization during action potential cardiac conduction transport ion transport transmembrane transport
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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