Invitrogen

Calbindin D28K Polyclonal Antibody

Calbindin D28K Antibody in Western Blot (WB)

FIGURE: 1 / 8

Calbindin D28K Antibody (PA1-931) in WB

Western blot analysis was performed using whole cell extracts (30 µg) of Mouse Brain (Lane 1), Rat Brain (Lane 2), IMR-32 (Lane 3), SH-SY5Y (Lane 4) and U-2 OS (Lane 5).The blots were probed with Anti-Calbindin D-28K Rabbit Polyclonal Antibody (Product # PA1-931, 2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4µg/mL 1:2500 dilution). A ~28 kDa band corresponding to Calbindin D-28K was observed across tissues and cell lines tested. Known... View More

Calbindin D28K Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

Calbindin D28K Antibody in Immunocytochemistry (ICC/IF)

Calbindin D28K Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

Product Details

PA1-931

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1 µg/mL

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

ELISA (ELISA)

Assay-dependent

Immunoprecipitation (IP)

5 µg/mL

Neutralization (Neu)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

28 kDa calbindin-D protein purified from rat kidney.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 15mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

AB_2068509

Product Specific Information

PA1-931 detects calbindin D-28k protein in rat samples.

PA1-931 has successfully been used in Western blot , ELISA, immunoprecipitation and immunohistochemical procedures. By Western blot, this antibody detects a 28 kDa protein representing calbindin D from rat cerebellum and rat kidney extract. Immunohistochemical staining on paraffin sections demonstrates that calbindin D is localized to the cytoplasm in epithelial cells of distal tubules in human kidney cortex after staining with PA1-931.

The PA1-931 immunizing protein corresponds to the purified 28 kDa calbindin-D protein from rat kidney.

Reconstitute in 100 µL PBS to create a stock of 1 mg/mL.

Target Information

Calbindin-D-28K (also termed vitamin D-dependent calcium-binding protein, or cholecalcin), is a highly conserved 28kDa calcium binding protein, with broad tissue distribution. It belongs, together with calmodulin, S-100, parvalbumin, troponin C and other proteins, to a family of low molecular weight calcium-binding proteins (CaBPs). These CaBPs have homologous primary structures, which contain conserved polypeptide folds of the EF-hand type for Ca2+ binding. Calbindin-D-28K is found predominantly in subpopulations of central and peripheral nervous system neurons, and in certain epithelial nervous system neurons, and in certain epithelial cells involved in Ca2+ transport such as distal tubular cells and cortical collecting tubules of the kidney, and in enteric neuroendocrine cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Social memory deficit caused by dysregulation of the cerebellar vermis.

Nature communications

Chao OY,Pathak SS,Zhang H,Augustine GJ,Christie JM,Kikuchi C,Taniguchi H,Yang YM

PA1-931 was used in Immunohistochemistry to suggest that the cerebellum organizes the neural matrix necessary for social recognition memory.

Tue Sep 26 00:00:00 UTC 2023

Lycii radicis cortex inhibits glucocorticoid‑induced bone loss by downregulating Runx2 and BMP‑2 expression.

International journal of molecular medicine

Lee B,Hong S,Kim M,Kim EY,Park HJ,Jung HS,Kim JH,Sohn Y

PA1-931 was used in Immunohistochemistry (Paraffin) to to suggest that the use of LRC alleviates GC‚induced secondary osteoporosis.

Sun Aug 01 00:00:00 UTC 2021

Lycii radicis cortex inhibits glucocorticoid‑induced bone loss by downregulating Runx2 and BMP‑2 expression.

International journal of molecular medicine

Lee B,Hong S,Kim M,Kim EY,Park HJ,Jung HS,Kim JH,Sohn Y

PA1-931 was used in Immunohistochemistry (Paraffin) to to suggest that the use of LRC alleviates GC‚induced secondary osteoporosis.

Sun Aug 01 00:00:00 UTC 2021

Conditional Deletion of Activating Rearranged During Transfection Receptor Tyrosine Kinase Leads to Impairment of Photoreceptor Ribbon Synapses and Disrupted Visual Function in Mice.

Frontiers in neuroscience

Peng WH,Liao ML,Huang WC,Liu PK,Levi SR,Tseng YJ,Lee CY,Yeh LK,Chen KJ,Chien CL,Wang NK

Published figure using Calbindin D28K polyclonal antibody (Product # PA1-931) in Immunocytochemistry

Fri May 24 00:00:00 UTC 2024

Conditional Deletion of Activating Rearranged During Transfection Receptor Tyrosine Kinase Leads to Impairment of Photoreceptor Ribbon Synapses and Disrupted Visual Function in Mice.

Frontiers in neuroscience

Peng WH,Liao ML,Huang WC,Liu PK,Levi SR,Tseng YJ,Lee CY,Yeh LK,Chen KJ,Chien CL,Wang NK

Published figure using Calbindin D28K polyclonal antibody (Product # PA1-931) in Immunocytochemistry

Fri May 24 00:00:00 UTC 2024

Targeting inhibitory cerebellar circuitry to alleviate behavioral deficits in a mouse model for studying idiopathic autism.

Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology

Chao OY,Marron Fernandez de Velasco E,Pathak SS,Maitra S,Zhang H,Duvick L,Wickman K,Orr HT,Hirai H,Yang YM

PA1-931 was used in Immunohistochemistry to highlight complex mechanisms converging onto the cerebellar dysfunction in the phenotypic model and provide effective strategies for potential therapies of ASD.

Mon Jun 01 00:00:00 UTC 2020

Anti-Yo antibody uptake and interaction with its intracellular target antigen causes Purkinje cell death in rat cerebellar slice cultures: a possible mechanism for paraneoplastic cerebellar degeneration in humans with gynecological or breast cancers.

PloS one

Greenlee JE,Clawson SA,Hill KE,Wood B,Clardy SL,Tsunoda I,Carlson NG

PA1-931 was used in blocking or activating experiment to investigate anit-Yo antibodies in Purkinje cell death

Mon Jan 18 00:00:00 UTC 2016

PloS one

Greenlee JE,Clawson SA,Hill KE,Wood B,Clardy SL,Tsunoda I,Carlson NG

PA1-931 was used in blocking or activating experiment to investigate anit-Yo antibodies in Purkinje cell death

Mon Jan 18 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: Calbindin; calbindin 1, (28kD); calbindin 1, 28 kD; calbindin 1, 28kDa; Calbindin D28; calbindin-28K; calbindin-D (28k); CalbindinD28K; cerebellar Ca-binding protein spot 35 protein; cerebellar Ca-binding protein, spot 35 protein; D-28K; PCD-29; RTVL-H protein; Spot 35 protein; Vitamin D-dependent calcium-binding protein, avian-type

View more View less

Gene Aliases: Brain-2; CAB27; CaBP28K; CALB; Calb-1; CALB1; CB; D-28K

View more View less

UniProt ID: (Human) P05937, (Rat) P07171, (Mouse) P12658

View more View less

Entrez Gene ID: (Human) 793, (Rat) 83839, (Mouse) 12307

View more View less

Function(s)

vitamin D binding calcium ion binding protein binding zinc ion binding metal ion binding

Process(es)

learning or memory short-term memory long-term memory locomotory behavior retina layer formation metanephric part of ureteric bud development vitamin D metabolic process regulation of synaptic plasticity regulation of cytosolic calcium ion concentration cellular response to organic substance metanephric collecting duct development metanephric distal convoluted tubule development metanephric connecting tubule development calcium ion homeostasis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

Calbindin D28K Polyclonal Antibody

Calbindin D28K Antibody (PA1-931) in WB

Product Details

PA1-931

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!