Invitrogen

Calcium Sensing Receptor Polyclonal Antibody

2 Published Figures

13 References

View all (23) Calcium Sensing Receptor antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (4)
Published Figures (2)

Calcium Sensing Receptor Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 6

Calcium Sensing Receptor Antibody (PA1-934A) in ICC/IF

Immunofluorescent analysis of Calcium Sensing Receptor (green) showing staining in the cytoplasm and nucleus of PC12 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Calcium Sensing Receptor polyclonal ant... View More

Calcium Sensing Receptor Antibody in Western Blot (WB)

Calcium Sensing Receptor Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

PA1-934A

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:10-1:100

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues A(12) L A W H S S A Y G P D Q R A Q(27) of rat CaSR.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2071477

Product Specific Information

PA1-934A detects calcium sensing receptor (CaSR) from human and rat tissues.

PA1-934A has been successfully used in Western blot and ICC/IF procedures. By Western blot, this antibody detects an ~120 kDa protein representing CaSR from HEK393 cells and rat brain homogenate.

The PA1-934A immunizing peptide corresponds to amino acid residues 12-27 from rat CaSR. This peptide (Cat.# PEP-009) is available for use in neutralization and control experiments.

Target Information

Senses changes in the extracellular concentration of calcium ions. The activity of this receptor is mediated by a G- protein that activates a phosphatidylinositol-calcium second messenger system.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Calcium-Sensing Receptor Expression in Breast Cancer.

International journal of molecular sciences

Busic-Pavlek I,Dumic-Cule I,Kovacevic L,Milosevic M,Delimar P,Korsa L,Marusic Z,Prutki M

Published figure using Calcium Sensing Receptor polyclonal antibody (Product # PA1-934A) in Immunohistochemistry (Paraffin)

Thu Jul 20 00:00:00 UTC 2023

Calcium-Sensing Receptor Contributes to Hyperoxia Effects on Human Fetal Airway Smooth Muscle.

Frontiers in physiology

Roesler AM,Ravix J,Bartman CM,Patel BS,Schiliro M,Roos B,Nesbitt L,Pabelick CM,Martin RJ,MacFarlane PM,Prakash YS

PA1-934A was used in Immunocytochemistry-immunofluorescence to demonstrate functional CaSR in developing ASM that contributes to hyperoxia-induced contractility and remodeling that may be relevant to perinatal airway disease.

Tue May 11 00:00:00 UTC 2021

Calcium-Sensing Receptor Contributes to Hyperoxia Effects on Human Fetal Airway Smooth Muscle.

Frontiers in physiology

Roesler AM,Ravix J,Bartman CM,Patel BS,Schiliro M,Roos B,Nesbitt L,Pabelick CM,Martin RJ,MacFarlane PM,Prakash YS

Tue May 11 00:00:00 UTC 2021

Calcium sensing receptor in developing human airway smooth muscle.

Journal of cellular physiology

Roesler AM,Wicher SA,Ravix J,Britt RD,Manlove L,Teske JJ,Cummings K,Thompson MA,Farver C,MacFarlane P,Pabelick CM,Prakash YS

PA1-934A was used in Immunocytochemistry-immunoflourescence to demonstrate functional Ca2+ ([Ca2+ ]o ) sensing receptor (CaSR) in developing ASM that modulates airway contractility and proliferation.

Thu Aug 01 00:00:00 UTC 2019

Evidence for a regulated Ca²⁺ entry in proximal tubular cells and its implication in calcium stone formation.

Journal of cell science

Ibeh CL,Yiu AJ,Kanaras YL,Paal E,Birnbaumer L,Jose PA,Bandyopadhyay BC

PA1-934A was used in Immunohistochemistry to show the existence of a regulated transcellular Ca2+ entry pathway in luminal membrane proximal tubule cells induced by Ca2+ sensing receptor-mediated activation of transient receptor potential canonical 3 channels.

Tue Apr 30 00:00:00 UTC 2019

Evidence for a regulated Ca²⁺ entry in proximal tubular cells and its implication in calcium stone formation.

Journal of cell science

Ibeh CL,Yiu AJ,Kanaras YL,Paal E,Birnbaumer L,Jose PA,Bandyopadhyay BC

Tue Apr 30 00:00:00 UTC 2019

Correlation between Choline Peak at MR Spectroscopy and Calcium-Sensing Receptor Expression Level in Breast Cancer: A Preliminary Clinical Study.

Molecular imaging and biology

Baio G,Rescinito G,Rosa F,Pace D,Boccardo S,Basso L,Salvi S,Calabrese M,Truini M,Neumaier CE

PA1-934A was used in immunohistochemistry - paraffin section to correlate choline with the CaSR expression surgical breast specimens

Sat Aug 01 00:00:00 UTC 2015

Decreased parathyroid Klotho expression is associated with persistent hyperparathyroidism after kidney transplantation.

Transplantation proceedings

Hong YA,Choi DE,Lim SW,Yang CW,Chang YK

PA1-934A was used in immunohistochemistry to study persistent hypoparathyroidism in kidney transplant patients and the role of reduced parathyroid expression of Klotho

Tue Oct 01 00:00:00 UTC 2013

In vivo imaging of human breast cancer mouse model with high level expression of calcium sensing receptor at 3T.

European radiology

Baio G,Fabbi M,Emionite L,Cilli M,Salvi S,Ghedin P,Prato S,Carbotti G,Tagliafico A,Truini M,Neumaier CE

PA1-934A was used in immunohistochemistry to evaluate an in vivo breast cancer cell imaging technique

Thu Mar 01 00:00:00 UTC 2012

A novel germline inactivating mutation in the CASR gene in an Italian kindred affected by familial hypocalciuric hypercalcemia.

European journal of endocrinology

Falchetti A,Gozzini A,Terranegra A,Soldati L,Vezzoli G,Leoncini G,Giusti F,Franceschelli F,Masi L,Tanini A,Cavalli L,Brandi ML

PA1-934A was used in western blot to study a novel germline CASR mutation in a case of familial hypocaliuric hypercalcemia in an Italian family

Tue May 01 00:00:00 UTC 2012

Establishment and characterization of a novel cell line derived from a human small cell lung carcinoma that secretes parathyroid hormone, parathyroid hormone-related protein, and pro-opiomelanocortin.

Human cell

Ishikawa M,Kimura K,Tachibana T,Hashimoto H,Shimojo M,Ueshiba H,Tsuboi K,Shibuya K,Yoshino G

PA1-934A was used in immunohistochemistry to characterize a novel hormone-secreting cell line derived from human small cell lung carcinoma

Sat May 01 00:00:00 UTC 2010

Regulation of mouse lung development by the extracellular calcium-sensing receptor, CaR.

The Journal of physiology

Finney BA,del Moral PM,Wilkinson WJ,Cayzac S,Cole M,Warburton D,Kemp PJ,Riccardi D

PA1-934A was used in immunohistochemistry to investigate the role of calcium-sensing receptor in mouse lung development

Mon Dec 15 00:00:00 UTC 2008

Normocalcemic primary hyperparathyroidism in patients with recurrent kidney stones: pathological analysis of parathyroid glands.

Virchows Archiv : an international journal of pathology

Yang AH,Hsu CW,Chen JY,Tseng LM,Won GS,Lee CH

PA1-934A was used in immunohistochemistry to study the abnormalities in parathyroid tissues from patients with normocalcemic primary hyperparathyroidism

Sat Jul 01 00:00:00 UTC 2006

The calcium sensing receptor and its alternatively spliced form in keratinocyte differentiation.

The Journal of biological chemistry

Oda Y,Tu CL,Pillai S,Bikle DD

PA1-934A was used in western blot to study the functional properties of the calcium-sensing receptor and its alternatively spliced form in keratinocyte differentiation

Fri Sep 04 00:00:00 UTC 1998

Bioinformatics

Protein Aliases: Ca sensing receptor; calcium-sensing receptor; Calcium-sensing receptor (hypocalciuric hypercalcemia 1 severe neonatal hyperparathyroidism); Calcium-sensing receptor (hypocalciuric hypercalcemia 1, severe neonatal hyperparathyroidism); CaR; CaSR; CaSR antibody; Extracellular calcium-sensing receptor; parathyroid Ca(2+)-sensing receptor 1; Parathyroid cell calcium-sensing receptor; Parathyroid cell calcium-sensing receptor 1; PCaR1

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Gene Aliases: CAR; CASR; EIG8; FHH; FIH; GPRC2A; HHC; HHC1; HYPOC1; NSHPT; PCAR1

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UniProt ID: (Human) P41180, (Rat) P48442

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Entrez Gene ID: (Human) 846, (Rat) 24247

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Function(s)

phosphatidylinositol phospholipase C activity G-protein coupled receptor activity protein binding magnesium ion binding integrin binding calcium ion binding drug binding polyamine binding protein kinase binding ion channel binding

Process(es)

ossification detection of calcium ion cellular calcium ion homeostasis G-protein coupled receptor signaling pathway chemosensory behavior anatomical structure morphogenesis calcium ion import response to ischemia calcium ion transport apoptotic process adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway phospholipase C-activating G-protein coupled receptor signaling pathway JNK cascade positive regulation of cell proliferation response to metal ion positive regulation of gene expression response to organic cyclic compound positive regulation of insulin secretion positive regulation of ATPase activity cellular response to hepatocyte growth factor stimulus vasodilation positive regulation of vasoconstriction branching morphogenesis of an epithelial tube positive regulation of positive chemotaxis response to calcium ion regulation of calcium ion transport fat pad development cellular response to vitamin D cellular response to glucose stimulus cellular response to low-density lipoprotein particle stimulus cellular response to hypoxia response to fibroblast growth factor positive regulation of calcium ion import cellular response to peptide

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Calcium Sensing Receptor Polyclonal Antibody

Calcium Sensing Receptor Antibody (PA1-934A) in ICC/IF

Product Details

PA1-934A

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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