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Invitrogen
Caldesmon Polyclonal Antibody
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Caldesmon Antibody (PA5-78906) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of Caldesmon in SiHa cells using Caldesmon Polyclonal Antibody (Product # PA5-78906). Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and incubated with the primary antibody at 5 µg/mL. DyLight 488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:500 di... View More
Product Details
PA5-78906
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived human Caldesmon recombinant protein (Position: M1-E120).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.01mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2746022
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human U20S whole cell, human A549 whole cell, human HepG2 whole cell, human Hela whole cell, human CACO-2 whole cell, mouse NIH/3T3 whole cell. IHC: mouse intestine tissue, rat intestine tissue, human intestinal cancer tissue. ICC/IF: SiHa cell. Flow: U87 cell.
Target Information
Caldesmon is a developmentally regulated protein involved in smooth muscle and non muscle contraction. Two closely related variants of human caldesmon have been identified which differ in their electrophorectic mobility and cellular distribution. The h caldesmon variant (120 - 150 kDa) is predominantly expressed in smooth muscle whereas l caldesmon (70 - 80 kDa) is found in non-muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Caldesmon; CDM; h-caldesmon; L-caldesmon; MGC21352; Non-muscle caldesmon; OTTHUMP00000207950; OTTHUMP00000207953; OTTHUMP00000207954; OTTHUMP00000207955; OTTHUMP00000214473; testis secretory sperm-binding protein Li 227n
Gene Aliases: 4833423D12Rik; AI195384; AV071549; AW536160; C920027I18Rik; CAD; CALD1; CDM; H-CAD; HCAD; L-CAD; LCAD; NAG22
UniProt ID: (Human) Q05682, (Rat) Q62736
Entrez Gene ID: (Human) 800, (Rat) 25687, (Mouse) 109624
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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