Invitrogen

Calmodulin Monoclonal Antibody (2D1)

2 Published Figures

13 References

View all (15) Calmodulin antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (10)
Published Figures (2)

Calmodulin Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 12

Calmodulin Antibody (MA3-917) in ICC/IF

Immunofluorescent analysis of Calmodulin using Calmodulin Monoclonal antibody (2D1) (Product # MA3-917) shows staining in HeLa cells. Calmodulin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Calmodulin (Produ... View More

Calmodulin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Calmodulin Antibody in Flow Cytometry (Flow)

Calmodulin Antibody in Immunohistochemistry (IHC)

Calmodulin Antibody in Western Blot (WB)

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Product Details

MA3-917

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

View 6 publications 6 publications

Immunohistochemistry (IHC)

1:20

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20

Immunocytochemistry (ICC/IF)

1:50

View 2 publications 2 publications

Flow Cytometry (Flow)

2 µg / 10^6 cells

ELISA (ELISA)

Assay-dependent

Neutralization (Neu)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bacteria, Bovine, Chicken, Human, Mouse, Rat

Published species

Algae, Fungi, Human, Mouse, Plant, Rat, Shrew

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2D1

Immunogen

Calmodulin purified from Dictyostelium discoideum.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2069438

Product Specific Information

MA3-917 detects calmodulin from human, bovine, chicken, Chlamydomonas, Dictyostelium, mouse and rat samples. This antibody does not detect parvalbumin, tropinin, S-100, or myosin light chain kinase (MLCK).

MA3-917 has been successfully used in Western blot, immunocytochemistry and ELISA procedures. By Western blot, this antibody detects a 17 kDa protein representing calmodulin from Dictyostelium cell lysate. Addition of EGTA to buffers completely inhibits antibody staining. Immunohistochemical staining of calmodulin in Dictyostelium cells with MA3-917 results in staining of the contractile vacuoles.

The MA3-917 antigen is calmodulin purified from Dictostelium discoideum.

Target Information

Calmodulin mediates the control of a large number of enzymes, ion channels, aquaporins and other proteins by Ca2+. Among the enzymes to be stimulated by the calmodulin-Ca2+ complex are a number of protein kinases and phosphatases. Together with CCP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis. Calmodulin is a small, highly conserved calcium binding protein found in all eukaryotic cells. With the capacity to bind up to four calcium ions, this 17 kDa protein acts as an important intracellular receptor for regulatory calcium signals. As it binds calcium, calmodulin undergoes conformational changes which can increase its affinity for target proteins. It acts both directly, through interaction with key target enzymes, and indirectly, via specific kinases. Studies have found that calmodulin participates in the regulation of several biological processes including energy and biosynthetic metabolism, cell motility, exocytosis, cytoskeletal assembly, and intracellular modulation of both cAMP and calcium concentrations. Calmodulin is the archetype of the family of calcium-modulated proteins of which nearly 20 members have been found. They are identified by their occurrence in the cytosol or on membranes facing the cytosol and by a high affinity for calcium. Calmodulin contains 149 amino acids and has 4 calcium-binding domains. Its functions include roles in growth and the cell cycle as well as in signal transduction and the synthesis and release of neurotransmitters.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

IQCN disruption causes fertilization failure and male infertility due to manchette assembly defect.

EMBO molecular medicine

Dai J,Li Q,Zhou Q,Zhang S,Chen J,Wang Y,Guo J,Gu Y,Gong F,Tan Y,Lu G,Zheng W,Lin G

MA3-917 was used in Immunohistochemistry to identify the function of IQCN, highlight the relationship between the manchette assembly and fertilization, and provide a genetic marker and a therapeutic option for male-source TFF.

Wed Dec 07 00:00:00 UTC 2022

Expression of Calbindin-D28K in the Developing and Adult Mouse Cochlea.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

Liu W,Chen H,Zhu X,Yu H

MA3-917 was used in Immunohistochemistry-immunofluorescence to describe the expression pattern of Calbindin-D28K (CaBP28K) in the mouse cochlea from late embryonic (E) stages to the adulthood.

Mon Aug 01 00:00:00 UTC 2022

Extracellular matrix remodeling through endocytosis and resurfacing of Tenascin-R.

Nature communications

Dankovich TM,Kaushik R,Olsthoorn LHM,Petersen GC,Giro PE,Kluever V,Agüi-Gonzalez P,Grewe K,Bao G,Beuermann S,Hadi HA,Doeren J,Klöppner S,Cooper BH,Dityatev A,Rizzoli SO

MA3-917 was used in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Western Blot, Immunoprecipitation to conclude that the neuronal ECM can be remodeled frequently through mechanisms that involve endocytosis and recycling of ECM proteins.

Wed Dec 08 00:00:00 UTC 2021

eNOS-NO-induced small blood vessel relaxation requires EHD2-dependent caveolae stabilization.

PloS one

Matthaeus C,Lian X,Kunz S,Lehmann M,Zhong C,Bernert C,Lahmann I,Müller DN,Gollasch M,Daumke O

Published figure using Calmodulin monoclonal antibody (Product # MA3-917) in Western Blot

Wed Mar 11 00:00:00 UTC 2020

Misregulation of calcium-handling proteins promotes hyperactivation of calcineurin-NFAT signaling in skeletal muscle of DM1 mice.

Human molecular genetics

Ravel-Chapuis A,Bélanger G,Côté J,Michel RN,Jasmin BJ

MA3-917 was used in Western Blotting to show that calcium-CnA signaling is hyperactivated in DM1 muscle and that such hyperactivation represents a beneficial compensatory adaptation to the disease.

Thu Jun 15 00:00:00 UTC 2017

Comparison of the protein expression of calpain-1, calpain-2, calpastatin and calmodulin between gastric cancer and normal gastric mucosa.

Oncology letters

Liu B,Zhou Y,Lu D,Liu Y,Zhang SQ,Xu Y,Li W,Gu X

MA3-917 was used in Immunohistochemistry to investigate the expression of calpain-1, calpain-2, calpastatin and calmodulin in gastric cancer and uninvolved gastric mucosa tissues with immunohistochemistry.

Fri Sep 01 00:00:00 UTC 2017

The maintenance ability and Ca²⁺ availability of skeletal muscle are enhanced by sildenafil.

Experimental & molecular medicine

Huang M,Lee KJ,Kim KJ,Ahn MK,Cho CH,Kim DH,Lee EH

MA3917 was used in western blot to examine the effects of sildenafil on skeletal muscle

Fri Dec 09 00:00:00 UTC 2016

Anti-Yo antibody uptake and interaction with its intracellular target antigen causes Purkinje cell death in rat cerebellar slice cultures: a possible mechanism for paraneoplastic cerebellar degeneration in humans with gynecological or breast cancers.

PloS one

Greenlee JE,Clawson SA,Hill KE,Wood B,Clardy SL,Tsunoda I,Carlson NG

MA3-917 was used in blocking or activating experiment to investigate anit-Yo antibodies in Purkinje cell death

Mon Jan 18 00:00:00 UTC 2016

Drosophila pericentrin requires interaction with calmodulin for its function at centrosomes and neuronal basal bodies but not at sperm basal bodies.

Molecular biology of the cell

Galletta BJ,Guillen RX,Fagerstrom CJ,Brownlee CW,Lerit DA,Megraw TL,Rogers GC,Rusan NM

MA3-917 was used in western blot to analyze Drosophila pericentrin and its requirement for interaction with calmodulin for function at centrosomes and neuronal basal bodies but not sperm basal bodies

Mon Sep 15 00:00:00 UTC 2014

Serotonin 5-HT3 receptor-mediated vomiting occurs via the activation of Ca2+/CaMKII-dependent ERK1/2 signaling in the least shrew (Cryptotis parva).

PloS one

Zhong W,Hutchinson TE,Chebolu S,Darmani NA

MA3-917 was used in immunohistochemistry to study the mechanism for serotonin 5-HT3 receptor-mediated vomiting

Thu Mar 31 00:00:00 UTC 2016

Involvement of the plasma membrane Ca2+-ATPase in the short-term response of Arabidopsis thaliana cultured cells to oligogalacturonides.

Plant biology (Stuttgart, Germany)

Romani G,Bonza MC,Filippini I,Cerana M,Beffagna N,De Michelis MI

MA3-917 was used in western blot to study the mechanism for the effect of oligogalacturonide on Arabidopsis thaliana cultured cells

Tue Jun 29 00:00:00 UTC 2004

Calmodulin and the contractile vacuole complex in mitotic cells of Dictyostelium discoideum.

Journal of cell science

Zhu Q,Liu T,Clarke M

MA3-917 was used in immunocytochemistry to investigate the interaction between calmodulin and the contractile vacuole complex during the mitosis of Dictyostelium discoideum

Thu Apr 01 00:00:00 UTC 1993

Characterization of the calcium-binding contractile protein centrin from Tetraselmis striata (Pleurastrophyceae).

The Journal of protozoology

Coling DE,Salisbury JL

MA3-917 was used in western blot to investigate the biochemical properties of centrin purified from Tetraselmis striata

Tue Sep 01 00:00:00 UTC 1992

Bioinformatics

Protein Aliases: calcium binding gene, DD112; Calcium-binding protein Dd112; CALM 1; Calmodulin; Calmodulin 1 (phosphorylase kinase, delta); calmodulin 2 (phosphorylase kinase, delta); calmodulin 3 (phosphorylase kinase, delta); calmodulin 5; Calmodulin III; Calmodulin-1; Calmodulin-2; Calmodulin-3; Calmodulin-4; calmodulin-like protein (neoCaM); CaM; CAMI; DD312; epididymis secretory protein Li 72; LP7057 protein; Neo-calmodulin; NeoCaM; phosphorylase kinase delta; phosphorylase kinase subunit delta; phosphorylase kinase, delta subunit; prepro-calmodulin 1; prepro-calmodulin 2; prepro-calmodulin 3; skin calmodulin-related factor

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Gene Aliases: 1500001E21Rik; 2310037J09Rik; AI256814; AI327027; AI461935; AI649108; AL024000; AL024017; AY314008; BOS_11189; BOS_11588; BOS_17673; BOS_19653; CALM; CALM1; CALM2; CALM3; Calm4; CALML2; CAM; CAM1; CAM2; CAM3; CaMA; CAMB; CAMC; CAMI; CAMII; CAMIII; CPVT4; DD112; DD132; HEL-S-72; LQT14; LQT15; PHKD; PHKD2; PHKD3; R75142; RCJMB04_24e7; RNCAMIII; Scarf; Scarf2

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UniProt ID: (Chicken) P62149, (Human) P0DP25, (Bovine) P62157, (Rat) P0DP30, (Mouse) P0DP28, (Mouse) Q9JM83

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Entrez Gene ID: (Bovine) 326597, (Chicken) 395855, (Chicken) 396523, (Human) 801, (Bovine) 617095, (Bovine) 100297344, (Human) 805, (Bovine) 520277, (Human) 808, (Rat) 24242, (Mouse) 12313, (Rat) 50663, (Mouse) 12314, (Rat) 24244, (Mouse) 12315, (Mouse) 80796, (Mouse) 494124

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Function(s)

calcium ion binding protein binding protein domain specific binding titin binding thioesterase binding N-terminal myristoylation domain binding phospholipase binding ion channel binding protein phosphatase activator activity myosin binding Ras guanyl-nucleotide exchange factor activity adenylate cyclase binding inositol-1,4,5-trisphosphate 3-kinase activity ligand-gated ion channel activity protein kinase binding nitric-oxide synthase regulator activity type 3 metabotropic glutamate receptor binding protein serine/threonine kinase activator activity phosphatidylinositol 3-kinase binding calcium-dependent protein binding nitric-oxide synthase binding calcium channel regulator activity enzyme regulator activity protein N-terminus binding metal ion binding molecular_function

Process(es)

Performance Guarantee

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Calmodulin Monoclonal Antibody (2D1)

Calmodulin Antibody (MA3-917) in ICC/IF

Product Details

MA3-917

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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