Invitrogen

Calnexin Polyclonal Antibody

Calnexin Antibody in Immunocytochemistry (ICC/IF)

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Calnexin Antibody (PA5-34665) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of Calnexin was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Calnexin Polyclonal Antibody (Product # PA5-34665) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Calnexin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

Product Details

PA5-34665

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:10,000

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human Calnexin. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.16 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2552017

Product Specific Information

Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji, NIH-3T3, PC-12, Rat2.

Predicted reactivity: Mouse (93%), Rat (93%), Zebrafish (82%), Xenopus laevis (89%), Dog (94%), Pig (94%), Chicken (91%), Bovine (94%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Calnexin, also referred to as IP90, p88 and p90, is an approximately 90 kDa integral membrane protein of the endoplasmic reticulum (ER). Many resident ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multisubunit proteins. Studies indicate that calnexin associates with the major histocompatibility complex (MHC) class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin, but not with completed receptor complexes. It has been shown that calnexin is a chaperone that retains incompletely or improperly folded proteins in the ER. The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble ER resident proteins such as GRP78 and GRP94 and protein disulfide isomerase. Integral membrane ER resident proteins, like calnexin, often lack this KDEL sequence but contain positively charged cytosolic residues that ensure ER retention. Calnexin contains a large ER luminal domain (461 amino acids), a transmembrane segment (22 amino acids), and a cytoplasmic tail (89 amino acids).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Claudin-23 reshapes epithelial tight junction architecture to regulate barrier function.

Nature communications

Raya-Sandino A,Lozada-Soto KM,Rajagopal N,Garcia-Hernandez V,Luissint AC,Brazil JC,Cui G,Koval M,Parkos CA,Nangia S,Nusrat A

Published figure using Calnexin polyclonal antibody (Product # PA5-34665) in Western Blot

Thu Oct 05 00:00:00 UTC 2023

Protamine Sulfate Is a Potent Inhibitor of Human Papillomavirus Infection In Vitro and In Vivo.

Antimicrobial agents and chemotherapy

Young JM,Zine El Abidine A,Gómez-Martinez RA,Bondu V,Sterk RT,Surviladze Z,Ozbun MA

PA5-34665 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to demonstrate the strong inhibitory activity of the heparin-neutralizing drug protamine sulfate (PS) against HPV infection.

Tue Jan 18 00:00:00 UTC 2022

Protamine Sulfate Is a Potent Inhibitor of Human Papillomavirus Infection In Vitro and In Vivo.

Antimicrobial agents and chemotherapy

Young JM,Zine El Abidine A,Gómez-Martinez RA,Bondu V,Sterk RT,Surviladze Z,Ozbun MA

Tue Jan 18 00:00:00 UTC 2022

Exosomal RNF157 mRNA from prostate cancer cells contributes to M2 macrophage polarization through destabilizing HDAC1.

Frontiers in oncology

Guan H,Mao L,Wang J,Wang S,Yang S,Wu H,Sun W,Chen Z,Chen M

PA5-34665 was used in Western Blot to investigate the role of exosomal RNF157 mRNA in prostate cancer development.

Fri Oct 21 00:00:00 UTC 2022

Regulation of intestinal epithelial intercellular adhesion and barrier function by desmosomal cadherin desmocollin-2.

Molecular biology of the cell

Raya-Sandino A,Luissint AC,Kusters DHM,Narayanan V,Flemming S,Garcia-Hernandez V,Godsel LM,Green KJ,Hagen SJ,Conway DE,Parkos CA,Nusrat A

Published figure using Calnexin polyclonal antibody (Product # PA5-34665) in Western Blot

Thu Apr 15 00:00:00 UTC 2021

Deciphering the Role of Extracellular Vesicles Derived from ZIKV-Infected hcMEC/D3 Cells on the Blood-Brain Barrier System.

Viruses

Fikatas A,Dehairs J,Noppen S,Doijen J,Vanderhoydonc F,Meyen E,Swinnen JV,Pannecouque C,Schols D

PA5-34665 was used in Western Blotting to investigate the role of extracellular vesicles derived from ZIKV-infected human brain microvascular endothelial cells on the blood-brain barrier (BBB) system.

Thu Nov 25 00:00:00 UTC 2021

The D614G Mutation Enhances the Lysosomal Trafficking of SARS-CoV-2 Spike.

bioRxiv : the preprint server for biology

Guo C,Tsai SJ,Ai Y,Li M,Pekosz A,Cox A,Atai N,Gould SJ

PA5-34665 was used in Immunocytochemistry to show that these results are consistent with a lysosomal pathway of coronavirus biogenesis and raise the possibility that a common mechanism may underly the D614G mutation's effects on spike protein trafficking in infected cells and the accelerated entry of SARS-CoV-2 into uninfected cells.

Wed Dec 09 00:00:00 UTC 2020

Optimization of Morpholino Antisense Oligonucleotides Targeting the Intronic Repressor Element1 in Spinal Muscular Atrophy.

Molecular therapy : the journal of the American Society of Gene Therapy

Osman EY,Washington CW,Kaifer KA,Mazzasette C,Patitucci TN,Florea KM,Simon ME,Ko CP,Ebert AD,Lorson CL

PA534665 was used in western blot to test if Morpholino-modified antisense oligonucleotide alter SMN2 splicing

Thu Sep 01 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: Calnexin; FLJ26570; IP90; Major histocompatibility complex class I antigen-binding protein p88; p88; p90

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Gene Aliases: 1110069N15Rik; AI988026; CANX; CNX; D11Ertd153e; IP90; P90

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UniProt ID: (Human) P27824, (Rat) P35565, (Mouse) P35564

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Entrez Gene ID: (Human) 821, (Rat) 29144, (Mouse) 12330

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Function(s)

glycoprotein binding calcium ion binding protein binding carbohydrate binding apolipoprotein binding ionotropic glutamate receptor binding poly(A) RNA binding unfolded protein binding metal ion binding

Process(es)

aging synaptic vesicle endocytosis chaperone-mediated protein folding clathrin-mediated endocytosis protein folding antigen processing and presentation of peptide antigen via MHC class I protein secretion antigen processing and presentation of exogenous peptide antigen via MHC class II protein folding in endoplasmic reticulum

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Calnexin Polyclonal Antibody

Calnexin Antibody (PA5-34665) in ICC/IF

Product Details

PA5-34665

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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