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Invitrogen
Calpain 2 Polyclonal Antibody
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FIGURE: 1 / 10
Calpain 2 Antibody (PA5-17494) in WB
Western blot was performed using Anti-Calpain 2 Polyclonal Antibody (Product # PA5-17494) and a 76 kDa band corresponding to Calpain 2 was observed in C2C12, DU 145, NIH/3T3, A-431 and PC-3, but not in Jurkat and Daudi which are reported to be negative. Whole cell lysates (30 µg lysate) of Jurkat (Lane 1), C2C12 (Lane 2), DU 145 (Lane 3), NIH/3T3 (Lane 4), A-431 (Lane 5), PC-3 (Lane 6) and Daudi (Lane 7) were electrophoresed using NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose... View More
Product Details
PA5-17494
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to the human sequence of calpain 2 (large subunit)
Conjugate
Form
Liquid
Concentration
54 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10981526
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
Mu-Calpain, also known as Calpain-I, and m-calpain, also known as Calpain-II, are intracellular, calcium-dependent cysteine proteases. Mu-calpain has a micromolar sensitivity (thus the mu-) as compared to the millimolar calcium sensitivity of m-calpain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 80 kDa M-calpain subunit; Calcium-activated neutral proteinase 2; CALP80; calpain 2, (m/II) large subunit; calpain 2, large [catalytic] subunit; calpain 2, large subunit; Calpain large polypeptide L2; Calpain M-type; calpain, large polypeptide L2; Calpain-2 catalytic subunit; Calpain-2 large subunit; CANP 2; cysteine proteinase; M-calpain; m-calpain 80 kDa large subunit; m-calpain large subunit; Millimolar-calpain
Gene Aliases: AI326419; CANP2; CANPL2; CANPml; Capa-2; Capa2; CAPN2; m-calpain; m-calpin; mCANP
UniProt ID: (Human) P17655, (Mouse) O08529, (Rat) Q07009
Entrez Gene ID: (Human) 824, (Mouse) 12334, (Rat) 29154
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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