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Invitrogen
Calpain 2 Polyclonal Antibody
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Calpain 2 Antibody (PA5-78912) in ICC/IF
Immunocytochemistry analysis of Calpain 2 using anti-Calpain 2 antibody (Product # PA5-78912) . Calpain 2 was detected in a section of HeLa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-Calpain 2 antibody (Product # PA5-78912) overnight at 4°C. DyLight®488 Conjugated ... View More
Product Details
PA5-78912
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived human Calpain 2 recombinant protein (Position: R500-L700).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2746028
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human HepG2 whole cell, human A431 whole cell, human Hela whole cell, rat kidney tissue, rat brain tissue, mouse kidney tissue, mouse brain tissue. IHC: human intestinal cancer tissue, human lung cancer tissue, rat small intestine tissue. ICC/IF: HELA cell. Flow: U87 cell.
Target Information
Mu-Calpain, also known as Calpain-I, and m-calpain, also known as Calpain-II, are intracellular, calcium-dependent cysteine proteases. Mu-calpain has a micromolar sensitivity (thus the mu-) as compared to the millimolar calcium sensitivity of m-calpain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 80 kDa M-calpain subunit; Calcium-activated neutral proteinase 2; CALP80; calpain 2, (m/II) large subunit; calpain 2, large [catalytic] subunit; calpain 2, large subunit; Calpain large polypeptide L2; Calpain M-type; calpain, large polypeptide L2; Calpain-2 catalytic subunit; Calpain-2 large subunit; CANP 2; cysteine proteinase; M-calpain; m-calpain 80 kDa large subunit; m-calpain large subunit; Millimolar-calpain
Gene Aliases: AI326419; CANP2; CANPL2; CANPml; Capa-2; Capa2; CAPN2; m-calpain; m-calpin; mCANP
UniProt ID: (Human) P17655, (Mouse) O08529, (Rat) Q07009
Entrez Gene ID: (Human) 824, (Mouse) 12334, (Rat) 29154
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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