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Invitrogen
Calpain 2 Monoclonal Antibody (107-82)
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Calpain 2 Antibody (MA3-942) in ICC/IF
Immunofluorescent analysis of M-Calpain in MCF-7 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a M-Calpain monoclonal antibody (Product # MA3-942) at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). M-Calpain staining (green), F-Actin st... View More
Product Details
MA3-942
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Pig,
Rabbit,
Rat
Published species
Bovine,
Fish,
Human,
Pig,
Rabbit,
Yeast
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
107-82
Immunogen
Purified bovine skeletal muscle 80 kDa subunit of m-calpain.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2290793
Product Specific Information
MA3-942 detects the 80 kDa subunit of m-calpain in human platelets and erythrocytes, bovine platelets, heart and skeletal muscle, rat myoblasts, kidney, liver and spleen, pig cultured cells, and rabbit samples. This antibody does not cross-react with mu-calpain, n-calpain, calmodulin or calpastatin.
MA3-942 has been successfully used in Western blot, IF, immunohistochemistry, and ELISA procedures. By Western blot, this antibody detects an ~80 kDa protein representing m-calpain in bovine platelets. Immunocytochemical staining of m-calpain in LLC-PK1 cells with MA3-942 results in diffuse cytoplasmic staining. This product has not been shown to be effective in immunoprecipitation experiments.
The MA3-942 antigen is purified bovine skeletal muscle 80 kDa subunit of m-calpain. This antibody recognizes an epitope between amino acids 502-699 (domain III/IV) of human calpain.
Target Information
Mu-Calpain, also known as Calpain-I, and m-calpain, also known as Calpain-II, are intracellular, calcium-dependent cysteine proteases. Mu-calpain has a micromolar sensitivity (thus the mu-) as compared to the millimolar calcium sensitivity of m-calpain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Calcium-activated neutral proteinase 2; calpain 2, (m/II) large subunit; calpain 2, large [catalytic] subunit; calpain 2, large subunit; calpain II; Calpain large polypeptide L2; Calpain M-type; calpain, large polypeptide L2; Calpain-2 catalytic subunit; Calpain-2 large subunit; CANP 2; M-calpain; Millimolar-calpain
Gene Aliases: BOS_15643; CANP 2; CANP2; CANPL2; CANPml; CAPN2; mCANP
UniProt ID: (Bovine) Q27971, (Human) P17655, (Rat) Q07009
Entrez Gene ID: (Rabbit) 100009092, (Pig) 397393, (Bovine) 281662, (Human) 824, (Rat) 29154
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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