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Bethyl Laboratories
Calpain 7/CAPN7 Polyclonal Antibody
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Calpain 7/CAPN7 Antibody (A305-860A-T) in WB
Detection of human Calpain 7/CAPN7 by western blot. Samples: Whole cell lysate (5 µg) from K-562, Hep-G2, RKO, MCF-7, HeLa, and U2OS cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-Calpain 7/CAPN7 antibody A305-860A-M (lot A305-860A-M-1) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 75 seconds.
Product Details
A305-860A-T
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A region between residue 50 and 100 of human Calpain-7.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7.0 to 8.0
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Wet ice
Product Specific Information
Predicted reactivity by homology: Porcine.
Target Information
Calpain proteinases are defined currently as papain-like neutral proteases that are calcium activated. Calpains 1 and 2 are composed of a large subunit, which is proteolytically active, and a small subunit (also called calpain-4), or enhancer protein, that is not proteolytically active. The other calpain proteins identified to date (calpain 5, 6, 8, 9, 10, 11, 12, 13, 14, 15) are not known to require a small subunit. Domains in the large subunit include the amino terminal domain-I, the proteinase domain-II, domain-III, and the EF-hand domain-IV (domain-T in calpains 5 & 6, domain-N for calpain-7). Calpain-7 domain-N does not have the standard calpain EF hand domain for binding calcium, making it unclear whether it is calcium regulated. In place of the EF hand calcium-binding domain IV, calpain-7 has a sequence most closely resembling domain-III, with unknown function. Calpain-7 is widely distributed, found in most tissues analyzed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: calpain like protease; Calpain-7; homolog of Aspergillus Nidulans PALB; PalB homolog; PalBH
Gene Aliases: CALPAIN7; CAPN7; PALBH
UniProt ID: (Human) Q9Y6W3
Entrez Gene ID: (Human) 23473
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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