Proteintech

Calpastatin Polyclonal Antibody

Calpastatin Antibody in Immunocytochemistry (ICC/IF)

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Calpastatin Antibody (12250-1-AP) in ICC/IF

Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Calpastatin antibody (Product # 12250-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Calpastatin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Calpastatin Antibody in Western Blot (WB)

Calpastatin Antibody in Flow Cytometry (Flow)

Calpastatin Antibody in Immunoprecipitation (IP)

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Product Details

12250-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 1 publication 1 publication

Flow Cytometry (Flow)

Assay-dependent

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Calpastatin Fusion Protein Ag2897 (1-350 aa encoded by BC013579)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MNPTETKAV KTEPEKKSQS TKPKSLPKQA SDTGSNDAHN KKAVSRSAEQ QPSEKSTEPK TKPQDMISAG GESVAGITAI SGKPGDKKKE KKSLTPAVPV ESKPDKPSGK SGMDAALDDL IDTLGGPEET EEENTTYTGP EVSDPMSSTY IEELGKREVT IPPKYRELLA KKEGITGPPA DSSKPIGPDD AIDALSSDFT CGSPTAAGKK TEKEESTEVL KAQSAGTVRS AAPPQEKKRK VEKDTMSDQA LEALSASLGT RQAEPELDLR SIKEVDEAKA KEEKLEKCGE DDETIPSEYR LKPATDKDGK PLLPEPEEKP KPRSESELID ELSEDFDRSE CKEKPSKPTE K (1-350 aa encoded by BC013579)

Target Information

Calpastatin is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Asiatic Acid Prevents Cognitive Deficits by Inhibiting Calpain Activation and Preserving Synaptic and Mitochondrial Function in Rats with Kainic Acid-Induced Seizure.

Biomedicines

Lu CW,Lin TY,Pan TL,Wang PW,Chiu KM,Lee MY,Wang SJ

Wed Mar 10 00:00:00 UTC 2021

Calpain2 but not calpain1 mediated by calpastatin following glutamate-induced regulated necrosis in rat retinal neurons.

Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft

Wang S,Huang Y,Yan Y,Zhou H,Wang M,Liao L,Wang Z,Chen D,Ji D,Xia X,Liu F,Huang J,Xiong K

Tue Jan 01 00:00:00 UTC 2019

Calpain2 but not calpain1 mediated by calpastatin following glutamate-induced regulated necrosis in rat retinal neurons.

Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft

Wang S,Huang Y,Yan Y,Zhou H,Wang M,Liao L,Wang Z,Chen D,Ji D,Xia X,Liu F,Huang J,Xiong K

Tue Jan 01 00:00:00 UTC 2019

Calpain2 but not calpain1 mediated by calpastatin following glutamate-induced regulated necrosis in rat retinal neurons.

Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft

Wang S,Huang Y,Yan Y,Zhou H,Wang M,Liao L,Wang Z,Chen D,Ji D,Xia X,Liu F,Huang J,Xiong K

Tue Jan 01 00:00:00 UTC 2019

Calpain2 but not calpain1 mediated by calpastatin following glutamate-induced regulated necrosis in rat retinal neurons.

Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft

Wang S,Huang Y,Yan Y,Zhou H,Wang M,Liao L,Wang Z,Chen D,Ji D,Xia X,Liu F,Huang J,Xiong K

Tue Jan 01 00:00:00 UTC 2019

Decreased calpain activity in chronic myeloid leukemia impairs apoptosis by increasing survivin in myeloid progenitors and xiap1 in differentiating granulocytes.

Oncotarget

Huang W,Bei L,Hjort EE,Eklund EA

Tue Aug 01 00:00:00 UTC 2017

Quantitative proteomic analysis reveals that transmissible gastroenteritis virus activates the JAK-STAT1 signaling pathway.

Journal of proteome research

An K,Fang L,Luo R,Wang D,Xie L,Yang J,Chen H,Xiao S

Fri Dec 05 00:00:00 UTC 2014

Quantitative proteomic analysis reveals that transmissible gastroenteritis virus activates the JAK-STAT1 signaling pathway.

Journal of proteome research

An K,Fang L,Luo R,Wang D,Xie L,Yang J,Chen H,Xiao S

Fri Dec 05 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: Calpain inhibitor; Calpastatin; Sperm BS-17 component

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Gene Aliases: BS-17; CAST; PLACK

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UniProt ID: (Human) P20810

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Entrez Gene ID: (Human) 831

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Function(s)

protease binding endopeptidase inhibitor activity protein binding calcium-dependent cysteine-type endopeptidase inhibitor activity poly(A) RNA binding cadherin binding involved in cell-cell adhesion

Process(es)

liver development egg activation brain development myoblast fusion aging negative regulation of endopeptidase activity organ regeneration myoblast differentiation negative regulation of cell cycle arrest cell-cell adhesion negative regulation of type B pancreatic cell apoptotic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Calpastatin Polyclonal Antibody

Calpastatin Antibody (12250-1-AP) in ICC/IF

Product Details

12250-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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