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Invitrogen
Calpastatin Monoclonal Antibody (1F7E3D10)
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Calpastatin Antibody (MA3-944) in IHC (P)
Immunohistochemistry was performed on cancer biopsies of deparaffinized Human breast carcinoma tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse mon... View More
Product Details
MA3-944
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Pig,
Rat
Published species
Bovine,
Fish,
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
1F7E3D10
Immunogen
Purified bovine skeletal muscle 80 kDa subunit of m-Calpastatin.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2259758
Product Specific Information
MA3-944 detects calpastatin from bovine, human, pig and rat tissues and cells. This antibody does not cross-react with calpains or calmodulin.
MA3-944 has been successfully used in Western blot and immunocytochemical procedures. By Western blot, this antibody detects 150, 125, 90 and 70 kDa proteins from bovine skeletal muscle extract which have been suggested to be proteins translated from different start sites of the calpastatin gene. Immunocytochemical staining of calpastatin in porcine LLC-PK1 cells with MA3-944 results in diffuse cytoplasmic staining.
The MA3-944 antigen is purified bovine skeletal muscle 80 kDa subunit of m-calpastatin. This antibody recognizes an epitope between amino acid residues 543-673 (domain IV) of human calpastatin.
Target Information
Calpastatin is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Calpain inhibitor; Calpastatin; calpastatin isoform III; calpastatin type I; calpastatin type II; calpastatin type III; Sperm BS-17 component
Gene Aliases: BOS_8501; BS-17; CAST; PLACK
UniProt ID: (Human) P20810, (Rat) P27321
Entrez Gene ID: (Human) 831, (Bovine) 281039, (Pig) 397135, (Rat) 25403
liver development
egg activation
brain development
myoblast fusion
aging
negative regulation of endopeptidase activity
organ regeneration
myoblast differentiation
negative regulation of cell cycle arrest
cell-cell adhesion
negative regulation of type B pancreatic cell apoptotic process
negative regulation of peptidase activity
regulation of protein catabolic process
negative regulation of catalytic activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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