Invitrogen

Calpastatin Monoclonal Antibody (2G11D6)

3 Published Figures

9 References

View all (27) Calpastatin antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (3)
Published Figures (3)

Calpastatin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 6

Calpastatin Antibody (MA3-945) in IHC (P)

Immunohistochemistry was performed on cancer biopsies of deparaffinized Human breast carcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:1000 with a mouse mon... View More

Calpastatin Antibody in Immunocytochemistry (ICC/IF)

Calpastatin Antibody in Western Blot (WB)

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Product Details

MA3-945

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000

View 6 publications 6 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

Immunohistochemistry (Frozen) (IHC (F))

1:200-1:1,000

Immunocytochemistry (ICC/IF)

1:1,000

View 2 publications 2 publications

Product Specifications

Species Reactivity

Bovine, Dog, Human, Pig, Rabbit, Rat

Published species

Bovine, Dog, Fish, Human, Rabbit

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

2G11D6

Immunogen

Bovine skeletal muscle calpastatin.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites, PBS

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2259757

Product Specific Information

MA3-945 detects calpastatin from bovine, human, canine, pig, rabbit, and rat tissues and cells. This antibody does not cross-react with calpains or calmodulin.

MA3-945 has been successfully used in Western blot, immunohistochemistry, and immunocytochemical procedures. By Western blot, this antibody detects 150, 125, 90 and 70 kDa proteins which have been suggested to be proteins translated from different start sites of the calpastatin gene. Immunofluorescence staining of calpastatin in porcine LLC-PK1 cells with MA3-945 results in diffuse cytoplasmic staining and weak nuclear staining.

The MA3-945 antigen is bovine skeletal muscle calpastatin. This antibody recognizes an epitope found between amino acid residues 295-501 (domain II) of human calpastatin.

Target Information

Calpastatin is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Postmortem proteolysis in three muscles from growing and mature beef cattle.

Meat science

Cruzen SM,Paulino PV,Lonergan SM,Huff-Lonergan E

MA3-945 was used in western blot to study proteolysis and calpain/calpastatin in three postmortem muscles from young and mature beef cattle and the implications for meat tenderness

Sat Feb 01 00:00:00 UTC 2014

Isolation and characterization of mu-calpain, m-calpain, and calpastatin from postmortem muscle. I. Initial steps.

Journal of animal science

Camou JP,Mares SW,Marchello JA,Vazquez R,Taylor M,Thompson VF,Goll DE

MA3-945 was used in western blot to identify and characterize calpains from postmortem muscle

Sat Dec 01 00:00:00 UTC 2007

Purification and characterization of calpain and calpastatin from rainbow trout, Oncorhynchus mykiss.

Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology

Saito M,Li H,Thompson VF,Kunisaki N,Goll DE

MA3-945 was used in western blot to purify and characterize the calpain and calpastatin from rainbow trout

Sun Apr 01 00:00:00 UTC 2007

Calpain is activated in degenerating photoreceptors in the rd1 mouse.

Journal of neurochemistry

Paquet-Durand F,Azadi S,Hauck SM,Ueffing M,van Veen T,Ekström P

Published figure using Calpastatin monoclonal antibody (Product # MA3-945) in Western Blot

Wed Feb 01 00:00:00 UTC 2006

Calpain is activated in degenerating photoreceptors in the rd1 mouse.

Journal of neurochemistry

Paquet-Durand F,Azadi S,Hauck SM,Ueffing M,van Veen T,Ekström P

Published figure using Calpastatin monoclonal antibody (Product # MA3-945) in Western Blot

Wed Feb 01 00:00:00 UTC 2006

Effect of nutrient restriction on calpain and calpastatin content of skeletal muscle from cows and fetuses.

Journal of animal science

Du M,Zhu MJ,Means WJ,Hess BW,Ford SP

MA3-945 was used in western blot to investigate the effect of nutrient restriction on calpain and calpastatin content of skeletal muscle from cows and fetuses

Wed Sep 01 00:00:00 UTC 2004

Effect of dietary protein on calpastatin in canine skeletal muscle.

Journal of animal science

Helman EE,Huff-Lonergan E,Davenport GM,Lonergan SM

MA3-945 was used in western blot to investigate the role of dietary protein on the expression of calpastatin in canine skeletal muscle

Mon Sep 01 00:00:00 UTC 2003

Subcellular localization and in vivo subunit interactions of ubiquitous mu-calpain.

The Journal of biological chemistry

Gil-Parrado S,Popp O,Knoch TA,Zahler S,Bestvater F,Felgenträger M,Holloschi A,Fernández-Montalván A,Auerswald EA,Fritz H,Fuentes-Prior P,Machleidt W,Spiess E

MA3-945 was used in immunocytochemistry to study the subcellular localization and in vivo subunit interactions of mu-calpain

Fri May 02 00:00:00 UTC 2003

Subcellular localization and in vivo subunit interactions of ubiquitous mu-calpain.

The Journal of biological chemistry

Gil-Parrado S,Popp O,Knoch TA,Zahler S,Bestvater F,Felgenträger M,Holloschi A,Fernández-Montalván A,Auerswald EA,Fritz H,Fuentes-Prior P,Machleidt W,Spiess E

MA3-945 was used in immunocytochemistry to study the subcellular localization and in vivo subunit interactions of mu-calpain

Fri May 02 00:00:00 UTC 2003

Fiber type-specific expression of major proteolytic systems in fast- to slow-transforming rabbit muscle.

American journal of physiology. Cell physiology

Sultan KR,Dittrich BT,Leisner E,Paul N,Pette D

MA3-945 was used in immunohistochemistry to study the importance of two major proteolytic systems in tranforming rabbit and rat muscles

Thu Feb 01 00:00:00 UTC 2001

Bioinformatics

Protein Aliases: Calpain inhibitor; Calpastatin; calpastatin isoform III; calpastatin type I; calpastatin type II; calpastatin type III; Sperm BS-17 component

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Gene Aliases: BOS_8501; BS-17; CAST; PLACK

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UniProt ID: (Human) P20810, (Rabbit) P08855, (Rat) P27321

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Entrez Gene ID: (Human) 831, (Bovine) 281039, (Dog) 479148, (Rabbit) 100009098, (Pig) 397135, (Rat) 25403

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Function(s)

protease binding endopeptidase inhibitor activity protein binding calcium-dependent cysteine-type endopeptidase inhibitor activity poly(A) RNA binding cadherin binding involved in cell-cell adhesion cysteine-type endopeptidase inhibitor activity

Process(es)

liver development egg activation brain development myoblast fusion aging negative regulation of endopeptidase activity organ regeneration myoblast differentiation negative regulation of cell cycle arrest cell-cell adhesion negative regulation of type B pancreatic cell apoptotic process negative regulation of peptidase activity regulation of protein catabolic process negative regulation of catalytic activity

Performance Guarantee

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Calpastatin Monoclonal Antibody (2G11D6)

Calpastatin Antibody (MA3-945) in IHC (P)

Product Details

MA3-945

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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