Proteintech

Calponin 1 Polyclonal Antibody

Calponin 1 Antibody in Immunocytochemistry (ICC/IF)

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Calponin 1 Antibody (13938-1-AP) in ICC/IF

Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using Calponin antibody (Product # 13938-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Calponin 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Calponin 1 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

Calponin 1 Antibody in Western Blot (WB)

Calponin 1 Antibody in Flow Cytometry (Flow)

Calponin 1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

13938-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 23 publications 23 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 9 publications 9 publications

Flow Cytometry (Flow)

Assay-dependent

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Calponin Fusion Protein Ag4989 (1-297 aa encoded by BC022015)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody raised against the full-length of human CNN1 may cross-react with CNN2 and CNN3.

Immunogen sequence: MSSAHFNRG PAYGLSAEVK NKLAQKYDHQ REQELREWIE GVTGRRIGNN FMDGLKDGII LCEFINKLQP GSVKKINEST QNWHQLENIG NFIKAITKYG VKPHDIFEAN DLFENTNHTQ VQSTLLALAS MAKTKGNKVN VGVKYAEKQE RKFEPGKLRE GRNIIGLQMG TNKFASQQGM TAYGTRRHLY DPKLGTDQPL DQATISLQMG TNKGASQAGM TAPGTKRQIF EPGLGMEHCD TLNVSLQMGS NKGASQRGMT VYGLPRQVYD PKYCLTPEYP ELGEPAHNHH AHNYYNSA (1-297 aa encoded by BC022015)

Target Information

Calponin, a thin filament associated protein is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. Calponin is expressed in smooth muscle and tissues containing significant amounts of smooth muscle. Two isoforms of calponin exist whose molecular weights are 34kDa and 29kDa. Expression of the 29kDa form is primarily restricted to muscle of the urogenital tract. The expression of calponin has also been demonstrated in myoepithelial cells from benign and malignant breast lesions. It stains smooth muscle, myoepithelial cells, myofibroblasts, keratinocytes and nerve fibers. It identifies myoepithelial cells in breast lesions, and helps differentiate breast collagenous spherulosis (positive) from adenoid cystic carcinoma. Adenoid cystic carcinoma in salivary gland tumors is calponin positive.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Basic calponin; calponin 1, basic, smooth muscle; calponin 1, smooth muscle; Calponin H1, smooth muscle; Calponin-1; calponins, basic; epididymis secretory protein Li 14

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Gene Aliases: CN; CNN1; CnnI; HEL-S-14; Sm-Calp; SMCC

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UniProt ID: (Human) P51911, (Mouse) Q08091, (Rat) Q08290

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Entrez Gene ID: (Human) 1264, (Mouse) 12797, (Rat) 65204

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Function(s)

actin binding protein binding calmodulin binding

Process(es)

actomyosin structure organization regulation of smooth muscle contraction

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Calponin 1 Polyclonal Antibody

Calponin 1 Antibody (13938-1-AP) in ICC/IF

Product Details

13938-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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