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Calponin 2 Antibody (MA5-49306) in WB
Western blot analysis of Calponin/CNN2 using Calponin/CNN2 antibody (Product # MA5-49306). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. Lane 1: chicken heart tissue lysates; Lane 2: chicken heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The ... View More
Product Details
MA5-49306
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Avian,
Chicken,
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
CP-93
Immunogen
Turkey gizzard calponin
Conjugate
Form
Lyophilized
Concentration
Conc. Not Determined
Storage buffer
ascites/1.2% sodium acetate with 2mg BSA
Contains
0.01mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3074964
Product Specific Information
Adding 1 mL of PBS buffer will yield a concentration of 100 µg/mL.
Positive Control - WB: chicken heart tissue, chicken heart tissue.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
The protein encoded by this gene, which can bind actin, calmodulin, troponin C, and tropomyosin, may function in the structural organization of actin filaments. The encoded protein could play a role in smooth muscle contraction and cell adhesion. Two transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Calponin H2, smooth muscle; Calponin-2; CLP2; H2-calponin; Neutral calponin
Gene Aliases: CNN2; RCJMB04_9b22
UniProt ID: (Human) Q99439
Entrez Gene ID: (Human) 1265, (Chicken) 100216000
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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