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Invitrogen
Calprotectin Monoclonal Antibody (MAC387)
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FIGURE: 1 / 5
Calprotectin Antibody (MA1-33972) in ICC/IF
Immunofluorescence analysis of Calprotectin was performed using log phase THP-1 and Raji cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Calprotectin Monoclonal Antibody (MAC387) (Product # MA1-33972) at 1:250 dilution in 0.1% BSA, incubated at 4 degr... View More
Product Details
MA1-33972
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Baboon,
Bovine,
Dog,
Cynomolgus monkey,
Deer,
Horse,
Cat,
Guinea pig,
Goat,
Human,
Mammal,
Mink,
Mouse,
Non-human primate,
Pig,
Rabbit,
Rhesus monkey,
Rat
Published species
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
MAC387
Immunogen
Human monocytes.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_1957578
Product Specific Information
For staining of paraffin sections, protein digestion pre-treatment (e.g. trypsin), 0.1% for 10 minutes or antigen retrieval using heat treatment is required.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The S100A8/A9 heterocomplex, also called MRP-8/MRP-14 complex or calprotectin, comprises 60% of the cytoplasmic protein fraction of circulating polymorphonuclear granulocytes and is also found in monocytes, macrophages and ileal tissue eosinophils. Peripheral blood monocytes carry the antigen extra- and intracellularly, neutrophils only intracellularly. The S100A8/A9 complex has antibacterial, antifungal, immunomodulating and antiproliferative effects. Besides this it is a potent chemotactic factor for neutrophils. Plasma concentrations are elevated in diseases associated with increased neutrophil activity, like inflammatory bowel disease. Granulocytes terminate their existence after transmigration through the intestinal wall. Therefore calprotectin is also detectable in feces. Elevated levels of calprotectin have been observed in body fluids such as plasma, saliva, gingival crevicular fluid, stools, and synovial fluid during infection and inflammatory conditions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: AI323541; B8Ag; BEE11; BEE22; calgranulin A; calgranulin B; Calgranulin-A; Calgranulin-B; calprotectin; Calprotectin L1H subunit; Calprotectin L1L subunit; CFAG; Chemotactic cytokine CP-10; chemotactic S100 protein; Cystic fibrosis antigen; intracellular calcium-binding protein (MRP14); Leukocyte L1 complex heavy chain; Leukocyte L1 complex light chain; macrophage migration inhibitory factor-related protein-8; Migration inhibitory factor-related protein 14; Migration inhibitory factor-related protein 8; MRP-14; MRP-8; Myeloid-related protein 14; Neutrophil cytosolic 23 kDa protein; Neutrophil cytosolic 7 kDa protein; p14; p23; P7; p8; Pro-inflammatory S100 cytokine; Protein S100-A8; Protein S100-A9; RNA-binding region containing protein 2-like; S100 calcium binding protein A8; S100 calcium binding protein A8 (calgranulin A); S100 calcium binding protein A9; S100 calcium binding protein A9 (calgranulin B); S100 calcium-binding protein A8; S100 calcium-binding protein A8 (calgranulin A); S100 calcium-binding protein A9; S100 calcium-binding protein A9 (calgranulin B); S100A8/S100A9; Urinary stone protein band A
Gene Aliases: 60B8AG; AI323541; AW546964; B8Ag; BEE22; BOS_2815; BOS_2834; CAGA; CAGB; CFAG; CGLA; CGLB; CP-10; EGK_21438; GAGB; L1AG; LIAG; MA387; MAC387; MIF; MRP-14; MRP14; MRP8; NIF; P14; P8; S100A8; S100A9
UniProt ID: (Bovine) P28782, (Human) P05109, (Bovine) P28783, (Rabbit) P50117, (Human) P06702, (Rat) P50115, (Mouse) P27005, (Mouse) P31725, (Rat) P50116
Entrez Gene ID: (Rabbit) 100008985, (Dog) 490461, (Rhesus monkey) 714740, (Bovine) 616818, (Pig) 100127488, (Cynomolgus monkey) 102121677, (Human) 6279, (Dog) 490463, (Bovine) 532569, (Pig) 100127489, (Rhesus monkey) 714690, (Rabbit) 100008704, (Cynomolgus monkey) 102122456, (Human) 6280, (Rat) 116547, (Mouse) 20201, (Mouse) 20202, (Rat) 94195
leukocyte migration involved in inflammatory response
autophagy
apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic process
neutrophil chemotaxis
innate immune response
positive regulation of inflammatory response
neutrophil aggregation
positive regulation of intrinsic apoptotic signaling pathway
acute inflammatory response
chronic inflammatory response
positive regulation of peptide secretion
inflammatory response
response to zinc ion
response to lipopolysaccharide
wound healing
response to ethanol
immune system process
regulation of translation
chemotaxis
peptidyl-cysteine S-nitrosylation
leukocyte chemotaxis
actin cytoskeleton reorganization
peptidyl-cysteine S-trans-nitrosylation
regulation of integrin biosynthetic process
regulation of inflammatory response
cytokine production
signal transduction
cell-cell signaling
astrocyte development
positive regulation of blood coagulation
positive regulation of cell growth
sequestering of zinc ion
chemokine production
defense response to bacterium
defense response to fungus
positive regulation of NF-kappaB transcription factor activity
regulation of cytoskeleton organization
cellular oxidant detoxification
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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