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Invitrogen
Calprotectin Monoclonal Antibody (MAC387)
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FIGURE: 1 / 5
Calprotectin Antibody (MA5-16808) in ICC/IF
Immunofluorescence analysis of Calprotectin was performed using log phase THP-1 and Raji cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Calprotectin Monoclonal Antibody (MAC387) (Product # MA5-16808) at 1:250 dilution in 0.1% BSA, incubated at 4 degr... View More
Product Details
MA5-16808
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Deer,
Horse,
Cat,
Guinea pig,
Goat,
Human,
Non-human primate,
Pig,
Rabbit,
Rat
Published species
Dog,
Human,
Non-human primate,
Pig
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
MAC387
Immunogen
Human monocytes
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2538291
Product Specific Information
This product requires protein digestion pre-treatment of paraffin sections using trypsin or pronase. A suggested positive control for immunohistochemical applications is human spleen. For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.
This product is a low-endotoxin, preservative-free formulation. Endotoxin level is <0.01EU/ug.
Mouse anti Human macrophages, clone MAC387 recognizes the L1 or Calprotectin molecule, an intracytoplasmic antigen comprised of a 12 kDa alpha chain and a 14 kDa beta chain.
Target Information
The S100A8/A9 heterocomplex, also called MRP-8/MRP-14 complex or calprotectin, comprises 60% of the cytoplasmic protein fraction of circulating polymorphonuclear granulocytes and is also found in monocytes, macrophages and ileal tissue eosinophils. Peripheral blood monocytes carry the antigen extra- and intracellularly, neutrophils only intracellularly. The S100A8/A9 complex has antibacterial, antifungal, immunomodulating and antiproliferative effects. Besides this it is a potent chemotactic factor for neutrophils. Plasma concentrations are elevated in diseases associated with increased neutrophil activity, like inflammatory bowel disease. Granulocytes terminate their existence after transmigration through the intestinal wall. Therefore calprotectin is also detectable in feces. Elevated levels of calprotectin have been observed in body fluids such as plasma, saliva, gingival crevicular fluid, stools, and synovial fluid during infection and inflammatory conditions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: AI323541; B8Ag; BEE11; BEE22; calgranulin A; calgranulin B; Calgranulin-A; Calgranulin-B; calprotectin; Calprotectin L1H subunit; Calprotectin L1L subunit; CFAG; Cystic fibrosis antigen; intracellular calcium-binding protein (MRP14); Leukocyte L1 complex heavy chain; Leukocyte L1 complex light chain; macrophage migration inhibitory factor-related protein-8; Migration inhibitory factor-related protein 14; Migration inhibitory factor-related protein 8; MRP-14; MRP-8; Myeloid-related protein 14; Neutrophil cytosolic 23 kDa protein; Neutrophil cytosolic 7 kDa protein; p14; p23; P7; p8; Protein S100-A8; Protein S100-A9; RNA-binding region containing protein 2-like; S100 calcium binding protein A8 (calgranulin A); S100 calcium binding protein A9 (calgranulin B); S100 calcium-binding protein A8; S100 calcium-binding protein A8 (calgranulin A); S100 calcium-binding protein A9; S100 calcium-binding protein A9 (calgranulin B); S100A8/S100A9; Urinary stone protein band A
Gene Aliases: 60B8AG; BOS_2815; BOS_2834; CAGA; CAGB; CFAG; CGLA; CGLB; CP-10; L1AG; LIAG; MA387; MAC387; MIF; MRP-14; MRP14; MRP8; NIF; P14; P8; S100A8; S100A9
leukocyte migration involved in inflammatory response
acute inflammatory response
chronic inflammatory response
positive regulation of peptide secretion
autophagy
activation of cysteine-type endopeptidase activity involved in apoptotic process
inflammatory response
response to zinc ion
neutrophil chemotaxis
response to lipopolysaccharide
wound healing
innate immune response
response to ethanol
positive regulation of inflammatory response
neutrophil aggregation
positive regulation of intrinsic apoptotic signaling pathway
apoptotic process
leukocyte chemotaxis
actin cytoskeleton reorganization
regulation of integrin biosynthetic process
cytokine production
regulation of translation
signal transduction
cell-cell signaling
astrocyte development
positive regulation of blood coagulation
positive regulation of cell growth
sequestering of zinc ion
chemokine production
peptidyl-cysteine S-trans-nitrosylation
defense response to bacterium
defense response to fungus
positive regulation of NF-kappaB transcription factor activity
regulation of cytoskeleton organization
cellular oxidant detoxification
peptidyl-cysteine S-nitrosylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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