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Invitrogen
Carbonic Anhydrase I Polyclonal Antibody
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FIGURE: 1 / 7
Carbonic Anhydrase I Antibody (PA5-78896) in ICC/IF
Immunocytochemistry analysis of CA1 using anti-CA1 antibody (Product # PA5-78896) . CA1 was detected in an immunocytochemical section of CACO-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 5 μg/mL rabbit anti-CA1 antibody (Product # PA5-78896) overnight at 4°C. DyLight®488 Conjugated G... View More
Product Details
PA5-78896
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human CA1 recombinant protein (Position: D9-F261).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2746012
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human HEL whole cell, rat spleen tissue, mouse spleen tissue. IHC: human adenocarcinoma of the colon cancer tissue, human spleen tissue, mouse colon tissue, rat colon tissue. ICC/IF: CACO-2 cell. Flow: HEL cell.
Target Information
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA1 is closely linked to CA2 and CA3 genes on chromosome 8, and it encodes a cytosolic protein which is found at the highest level in erythrocytes. Variants of this gene have been described in some populations. Multiple alternatively spliced variants, encoding the same protein, have been identified. Transcript variants of CA1 utilizing alternative polyA_sites have been described in literature.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CA I; CA-I; CA-I {ECO:0000250|UniProtKB:P00915}; ca1 {ECO:0000250|UniProtKB:P00915}; CAB; Carbonate dehydratase I; carbonate dehydratase I {ECO:0000250|UniProtKB:P00915}; Carbonic anhydrase 1; carbonic anhydrase 1 {ECO:0000250|UniProtKB:P00915}; Carbonic anhydrase B; Carbonic anhydrase I; carbonic anhydrase I {ECO:0000250|UniProtKB:P00915}; Cyanamide hydratase CA1; epididymis secretory protein Li 11
Gene Aliases: AW555628; CA-I; CA1; CAB; Car-1; Car1; HEL-S-11
UniProt ID: (Human) P00915, (Rat) B0BNN3, (Mouse) P13634
Entrez Gene ID: (Human) 759, (Rat) 310218, (Mouse) 12346
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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