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Carbonic Anhydrase I Antibody (PA5-94987) in WB
Western blot analysis of Carbonic Anhydrase I/CA1 in, Lane 1: human HEL whole cell lysates, Lane 2: rat spleen tissue lysates. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. After Electrophoresis, proteins were transferred to a nitrocellulose membrane at 1... View More
Product Details
PA5-94987
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Carbonic Anhydrase I (51-68aa SYNPATAKEIINVGHSFH).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide, 0.05mg thimerosal
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2806793
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human HEL whole cell, rat spleen tissue.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA1 is closely linked to CA2 and CA3 genes on chromosome 8, and it encodes a cytosolic protein which is found at the highest level in erythrocytes. Variants of this gene have been described in some populations. Multiple alternatively spliced variants, encoding the same protein, have been identified. Transcript variants of CA1 utilizing alternative polyA_sites have been described in literature.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CA-I; CA-I {ECO:0000250|UniProtKB:P00915}; ca1 {ECO:0000250|UniProtKB:P00915}; CAB; Carbonate dehydratase I; carbonate dehydratase I {ECO:0000250|UniProtKB:P00915}; Carbonic anhydrase 1; carbonic anhydrase 1 {ECO:0000250|UniProtKB:P00915}; Carbonic anhydrase B; Carbonic anhydrase I; carbonic anhydrase I {ECO:0000250|UniProtKB:P00915}; Cyanamide hydratase CA1; epididymis secretory protein Li 11
Gene Aliases: CA-I; CA1; CAB; Car1; HEL-S-11
UniProt ID: (Human) P00915, (Rat) B0BNN3
Entrez Gene ID: (Human) 759, (Rat) 310218
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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